Library

feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    ISSN: 1432-0533
    Keywords: CD45 ; Protein phosphotyrosine phosphatase ; Microglia ; Intracellular signaling ; Alzheimer's disease
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Both protein kinases and phosphoprotein phosphatases are important components of signal transduction systems in cells. Recent studies in Alzheimer's disease (AD) have shown abnormal protein phosphorylation in the cortex suggesting an alteration in these enzymes. In the present study, an antibody against CD45 was used to analyze the status of this protein phosphotyrosine phosphatase in AD. We studied and quantified the immunohistochemical and immunochemical distribution of this integral membrane protein in control and AD brain. We found that anti-CD45 immunostained the great majority of microglia, both resting and activated. These cells were Ricinus communis agglutinin I positive and glial fibrillary acidic protein and neurofilament negative. The AD frontal cortex showed a 35% (P〈0.01) increase in the number of anti-CD45 immunoreactive microglia as compared with controls. These results were consistent with the immunoblot quantification of CD45 immunoreactivity following native gel electrophoresis. In AD, 30% of the CD45-immunostained microglia were clustered in the neuritic plaques (about six per plaque) while the remaining 70% were scattered in the neuropil. The AD hippocampus showed an increase in CD45-immunoreactive microglia in the molecular layer of the dentte gyrus. At the ultrastructural level, CD45 immunoreactivity was localized exclusively to the plasma membrane of the microglia. The presence of the anti-CD45 immunoreactivity in microglia suggests the possibility that they may require the presence of CD45 as a cell surface receptor which may regulate cell function through modulation of intracellular signaling.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Acta neuropathologica 81 (1991), S. 428-433 
    ISSN: 1432-0533
    Keywords: Alzheimer's disease ; Biopsy ; Synapses ; Synaptophysin ; Immunoelectron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Alzheimer's disease (AD) is characterized by an extensive loss of neurons and synapses in the neocortex which correlates strongly with psychometric tests of dementia. To characterize the ultrastructural changes in presynaptic terminals in AD, we studied biopsy material from the frontal cortex. We also examined, at the ultrastructural level, abnormal neurites scattered in the AD neuropil and in the plaque region using sections from autopsy material immunolabeled with anti-synaptophysin. We found that, regardless of amyloid deposits, some presynaptic terminals were distended and contained swollen vesicles and dense bodies. These altered synaptic organelles were similar to those found in dystrophic neurites. The latter structures displayed synaptophysin immunoreactivity, mostly localized to outer membranes of synaptic vesicles and dense bodies. The present study supports the hypothesis of progressive synaptic pathology in AD neocortex and favors the notion that the dystrophic process originates from presynaptic terminals.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 3
    ISSN: 1432-0533
    Keywords: Key wordsα Synuclein ; Proteinase K ; Progressive ¶supranuclear palsy ; Corticobasal degeneration ; Non-amyloid β component
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract α-Synuclein is a presynaptic terminal protein that accumulates abnormally in plaques in Alzheimer’s disease (AD), in Lewy bodies in Lewy body disease (LBD) and in filamentous inclusions in multiple system atrophy. Since it has been previously shown that proteinase K or formic acid pretreatment enhances α-synuclein immunoreactivity in Lewy bodies and plaques, we hypothesized that the immunoreactivity in tangles, glial cells and Pick bodies might be revealed by such pretreatment. Brain sections from patients with AD, LBD, progressive supranuclear palsy (PSP), corticobasal degeneration (CBD) and Pick’s disease were pretreated with proteinase K or formic acid and immunostained with antibodies against the N-terminal, C-terminal or non-amyloid β component of AD amyloid (NAC) regions of α-synuclein. This study showed that after proteinase K (but not formic acid) pretreatment the anti-C terminus antibody immunostained neurofibrillary tangles of AD, PSP and CBD, and glial inclusions of PSP and CBD, as well as Pick bodies. Western blot analysis confirmed that in cases other than LBD, the anti-C terminus antibodies also recognized the native α-synuclein band and no cross-reactive bands were observed. In contrast, in LBD, after formic acid pretreatment with the anti-NAC antibody astroglial cells and granular neurons were immunostained. The N-terminal region antibody only recognized the lesions in LBD cases and not those of other neurodegenerative disorders. These results support the view that different fragments of α-synuclein might play an important role in the pathogenesis of several neurodegenerative disorders.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Water, air & soil pollution 85 (1995), S. 457-462 
    ISSN: 1573-2932
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Information on breeding waterfowl, habitat and food chains, gathered from acid-sensitive lakes in Ontario, was used to develop a model of effects of acid deposition on waterfowl and their response to predicted sulphur dioxide (SO2 emission reductions in eastern North America. The Waterfowl Acidification Response Modelling System (WARMS) is composed of an acidification model linked to fish and waterfowl models. WARMS uses pH, area, dissolved organic carbon, total phosphorus, and presence of fish to calculate estimates of pre-acidification, present and eventual steady-state values for pH, fish presence and waterfowl breeding parameters under proposed SO2 emission scenarios. We used WARMS to estimate chemical and biotic responses to scenarios simulated in three regions of Ontario where biomonitoring studies are underway. For pH and fish presence, WARMS predicts the greatest improvements in the highly damaged Sudbury region, slight improvements in Algoma, and that the strongest proposed emission reductions will be required to maintain current conditions in Muskoka. For waterfowl, species-specific differences are evident among regions. We discuss implications of these assessments of biological recovery for watersheds in eastern Canada.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Water, air & soil pollution 85 (1995), S. 451-456 
    ISSN: 1573-2932
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The biomonitoring program of Environment Canada examines food chains in small Ontario lakes to interpret ecological responses of waterfowl and their foods to changing acid deposition. Macroinvertebrates and fish were sampled in three acid-sensitive regions: Muskoka (1991; N=20), Algoma (1992; N=20), and Sudbury (1994; N=22). Small lakes (〈11 ha; important breeding habitat for waterfowl) were chosen to cover the range of pH in each region, and include those with and without fish. In all regions, macroinvertebrate taxonomic richness (particularly nekton and benthos) was greater in fishless lakes compared to lakes with fish. Among fishless lakes, taxonomic richness (especially benthos) was positively correlated with pH, although regional differences were evident. Previous studies near Sudbury have shown that several benthic groups have distribution and abundance patterns with respect to pH (Trichoptera, Ephemeroptera, Hirudinea, Amphipoda, and Gastropoda). Those patterns continue near Sudbury, and were also strongly apparent in Algoma. In all regions, the number of acid-sensitive taxa per lake is related to pH, and should increase as lakes recover from acidification. However, predicting macroinvertebrate responses to recovery must consider concurrent effects of fish, as they are a dominant factor structuring these communities.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 6
    ISSN: 1573-2932
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The Common Loon (Gavia immer) is a conspicuous and popular aquatic bird that inhabits large lakes (generally 〉10 ha) on Precambrian Shield across Canada. Because it relies on fish, it is a key bioindicator species linking acid precipitation to higher trophic levels in aquatic food chains. The Canadian Lakes Loon Survey (CLLS), a monitoring program involving volunteers, was initiated in 1981 to collect information on loon reproductive success, such as the number of loons nesting and the number of young they produce which survive to fledging, to help researchers determine whether loons were adversely affected by acid rain or human disturbance. Between 1987 and 1993, 4236 records for loons nesting on 1529 lakes in Ontario (historically the principal region of study) were received from volunteers. We used logistic regression to examine relationships between loon productivity (number of large young produced per pair for 721 lakes), lake area, and pH (from provincial and federal databases). After controlling for lake size, we determined that loons were less likely to nest on acid lakes (pH〈5.5), and when they did attempt to breed, their reproductive success was lower, in part due to the reduced survival of two-chick broods, presumably linked to reduced fish biomass. However, we found no conclusive evidence that breeding success exhibited any temporal trends in relation to pH over this time period. Continued monitoring by volunteers of loon reproduction on CLLS lakes will provide a reliable, costeffective method of assessing the long-term health of large, acid-sensitive lakes across Canada.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...