ISSN:
1432-2242
Keywords:
Key words Medicago sativa
;
RAPD
;
Cultivars
;
Genetic distance
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Alfalfa (Medicago sativa L.) is a forage legume of world-wide importance whose both allogamous and autotetraploid nature maximizes the genetic diversity within natural and cultivated populations. This genetic diversity makes difficult the discrimination between two related populations. We analyzed this genetic diversity by screening DNA from individual plants of eight cultivated and natural populations of M. sativa and M. falcata using the RAPD method. A high level of genetic variation was found within and between populations. Using five primers, 64 intense bands were scored as present or absent across all populations. Most of the loci were revealed to be highly polymorphic whereas very few population-specific polymorphisms were identified. From these observations, we adopted a method based on the Roger’s genetic distance between populations using the observed frequency of bands to discriminate populations pairwise. Except for one case, the between-population distances were all significantly different from zero. We have also determined the minimal number of bands and individuals required to test for the significance of between-population distances.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s001220050756
Permalink
