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  • 1
    ISSN: 1420-908X
    Keywords: Key words: Mast cells — Fc receptors — TNF-α— Gene regulation — RBL
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Objective: In the present study, we investigated signal transduction pathways involved in TNF-α gene expression and TNF-α secretion by mast cells stimulated through the high affinity IgE receptor (FcɛRI).¶Materials and Methods: TNF-α mRNA steady state levels and TNF-α secretion in the presence of specific pharmacological agents were monitored using rat basophilic leukemia cells (RBL-2H3) stimulated through FcɛRI. Relative amounts of TNF-α mRNA versus β-actin levels were quantified by RNase protection and RT-PCR assays. TNF-α secretion was measured by a current ELISA test.¶Results: We show that EGTA (5 mM) prevented TNF-α mRNA expression and TNF-α secretion in antigen-stimulated cells. The protein kinase C (PKC) inhibitor bisindolylmaleimide I substantially blocked TNF-α secretion at 2 μM but had only a marginal effect on TNF-α mRNA expression. The results were similar when PKC isoforms were depleted by long-term exposure to 100 nM phorbol ester (PMA). The PI 3-kinase inhibitor wortmannin blocked TNF-α secretion at low doses (EC50 = 13 nM), but only partially affected mRNA expression.¶Conclusions: Our results show that in FcɛRI-stimulated RBL-2H3 cells calcium mobilization, activation of PKC and PI 3-kinase are necessary for TNF-α secretion while for the increased TNF-α mRNA expression PKC activity is dispensable and PI 3-kinase activity only partially required.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1420-908X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Anatomy and embryology 21 (1903), S. 217-278 
    ISSN: 1432-0568
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Medical microbiology and immunology 47 (1904), S. 1-40 
    ISSN: 1432-1831
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Journal of fish biology 63 (2003), S. 0 
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: No significant differences were found in the time budget (time spent in feeding, moving and stationary), attack rate (number of feeding bouts min−1), and microhabitat use of juvenile (1+ years) littoral and pelagic brook charr Salvelinus fontinalis at 2 and 4 m depth, when restricted to feeding in pelagic enclosures. In contrast, fish of the littoral form allocated significantly more time than pelagic ones to feeding, moving and in total activity at 3 m depth. No significant differences were found in attack rate between the two forms at any given depth. Based on the mean for the water column (all depths pooled), however, fish of the littoral form executed a significantly higher attack rate than fish of the pelagic one. In multiple regressions analyses, the best predictors of time allocated to feeding and attack rate were the dummy variable ‘form’ (littoral individuals spend significantly more time in feeding than pelagic ones), light intensity at the surface (negative) and water temperature (positive), and explained 48 and 55% of these variations, respectively. Time allocated to moving was only explained by water temperature (negative) and explained 43% of the variation. Time in a stationary position was best explained by water temperature (negative) and light intensity at the surface (positive), explaining 52% of the variation. The results of this study support the hypothesis that littoral brook charr spend more energy in foraging than pelagic ones when restricted to feeding in the pelagic habitat, and thus that trophic diversification is adaptive in this species.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Freshwater biology 47 (2002), S. 0 
    ISSN: 1365-2427
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SUMMARY 1. The objective of this study was to evaluate the effects of zooplankton biomass (as a measure of density), fish biomass, light intensity and water temperature on the attack rate and swimming characteristics (i.e. swimming speed and angle of turn) of juvenile (1+) brook charr (Salvelinus fontinalis) in field enclosures. We used a portable underwater camera system in a series of pelagic enclosures to quantify the feeding behaviour of brook charr over a gradient of natural conditions.2. In simple linear or non-linear regression models we found (i) that attack rate and angle of turn were positively related to water temperature, (ii) that attack rate and swimming speed were positively related to zooplankton biomass and light intensity and (iii) that attack rate was positively related to swimming speed. In multiple regression models, fish biomass, light intensity and variance of the angle of turn accounted for 87% of the variation in attack rate. Light intensity and water temperature accounted for 86% of the variation in swimming speed. Fish gut fullness and attack rate accounted for 83% of the variation in the variance of the angle of turn executed by fish.3. The increase in the number of attacks as zooplankton biomass increases conforms to the general positive functional response observed in other fish species. Our results also support the hypothesis that swimming speed increases with prey biomass. We did not observe a plateau in attack rate as zooplankton biomass increased. As our experiments were performed under natural biotic and abiotic conditions, factors other than zooplankton biomass might affect or limit this response, such as water temperature and light intensity.4. Because zooplankton biomass was correlated with water temperature and light intensity, it was not possible to evaluate the independent contribution of these factors on the attack rate and swimming characteristics (swimming speeds and angle of turn) of brook charr. However, this study highlighted the impact of these factors on the feeding behaviour of juvenile brook charr when feeding in the pelagic habitat under natural conditions, and their importance in future models of optimal foraging and fish habitat quality.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background:  Although allergen-specific IgE content in serum can be determined immunochemically, little is known about the relationship between this parameter and the strength of the degranulation response upon allergen triggering.Objectives:  Analyse the degranulation capacity of immunochemically defined purified and serum IgE after challenge with anti-IgE or allergen using a rat mast cell line (RBL) transfected with the α-chain of the human high-affinity IgE receptor (FcɛRI).Methods:  Purified IgE specific for 4-hydroxy-3nitrophenylacetyl, purified IgE of unknown specificity, and sera from allergic patients sensitive to Dermatophagoides pteronyssinus and Dactylis glomerata were assessed. Degranulation was measured by a β-hexosaminidase release assay after anti-IgE or allergen-specific challenge.Results:  For purified monoclonal IgE a significant correlation (r = 0.97) was found between the proportion of bound allergen-specific IgE and the strength of the degranulation response. In contrast, no correlation (r = 0.27) was detected after sensitization with serum IgE.Conclusion:  Our studies demonstrate that mast cell activation mediated through IgE from allergic patients is a result of complex relationships that are not only dependent on allergen-specific IgE content but also relate to the capacity to efficiently sensitize and trigger the signalling responses that lead to degranulation.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background: Rat basophilic leukemia (RBL-2H3) cells are well characterized in terms of morphological and biochemical changes upon activation, and have been extensively used as a model system for studying the mechanisms of the immediate hypersensitivity reaction. To investigate whether overexpression of heat shock/stress proteins (HSP) is involved in the mast cell-dependent reactivity, we examined the adaptive responses of RBL-2H3 cells to classical stress conditions such as heat shock or oxidative injury produced by an aqueous extract of tobacco smoke.Methods: HSP were determined by flow cytometry and immunocytochemistry. Degranulation was confirmed as the release of β-hexosaminidase, determined spectrophotometrically, and by electron microscopy experiments.Results: We found that RBL-2H3 cells respond to heat shock or oxidative injury by the synthesis of both the inducible 72 kDa HSP (Hsp70), and the oxidation-specific 32 kDa heme oxygenase (HO)-1. Heat shock induced mainly Hsp70 in a cell growth-dependent manner, whereas oxidative stress induced mainly HO-1 in a cell growth-independent manner. However, heat shock or oxidative stress had no significant effects on degranulation.Conclusion: Stress-mediated synthesis of HSP was not associated with RBL-2H3 degranulation and likewise, degranulation did not induce HSP.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Forty-three patients allergic to mites and suffering mainly from asthma were recruited: 25 were mite-monosensitive and 18 were polysensitive, as determined by skin tests and specific serum IgE determinations with various allergens. In vitro spontaneous histamine release (SHR) by washed blood basophils was measured once or several times for each patient. Throughout this study, the mean periods of high and low mite-allergen exposure were defined on the basis of relative indoor humidity and temperature data. For the mite-monosensitive patients, there was a significant increase in mean SHR during the season of high mite-allergen exposure as compared to the months of lower mite-allergen presence (P 〈 0.002). No significant difference between mean SHR values was observed when comparing the monosensitive group during the season of high mite-allergen exposure with polysensitive patients (allergic to mite and pollen) during the period of exposure to both allergens. Differences in mean SHR reported here emphasize the positive relationship between intense allergen exposure and the in vitro SHR increase in blood basophils of mite-allergic patients.
    Type of Medium: Electronic Resource
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  • 10
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    Paris : Periodicals Archive Online (PAO)
    Etudes de linguistique appliquée. n.s.:35 (1979:juil./sept.) 5 
    ISSN: 0071-190X
    Topics: Linguistics and Literary Studies
    Notes: MÉLANGES DE DIDACTIQUE DES LANGUES
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