ISSN:
0001-1541
Keywords:
Chemistry
;
Chemical Engineering
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Vortex flow filtration experiments with bovine serum albumin (BSA) solutions and Arthrobacter simplex cell suspensions in a rotary membrane device are modeled in the pressure-dependent flux and mass-transfer-controlled regions. An expression for the concentration-dependent diffusion coefficients accounting for hydrodynamic and long-range potential interactions between rigid spherical macromolecules is used for BSA. A shear-induced diffusion coefficient, strongly concentration-dependent on the cell concentration, is assumed to describe the diffusion behavior of the cell suspensions. The adjusted model parameters for albumin solutions in the pressure-dependent region of flux predict the permeation flux behavior for A. Simplex cell suspensions. When the mass-transfer correlation is calculated using cell diffusion coefficients, based on a volumetric cell fraction that is around half of the average value of the calculated membrane surface fractions, the fluxes are estimated within less than 10.5% relative error. In the mass-transfer-controlled region of flux, the volume fractions of cells at the membrane surface are between 0.16 and 0.35, depending on bulk concentration and hydrodynamic conditions.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/aic.690410403
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