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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Annals of hematology 18 (1968), S. 114-122 
    ISSN: 1432-0584
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Annals of hematology 18 (1969), S. 367-375 
    ISSN: 1432-0584
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Annals of hematology 18 (1969), S. 311-316 
    ISSN: 1432-0584
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Annals of hematology 19 (1969), S. 347-350 
    ISSN: 1432-0584
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Conclusions Our studies showed that at very rapid cooling rates of more than 100° C. per second in liquid helium the platelets are damaged and after thawing do not circulate in the peripheral blood. We don't have any explanation for this. But it seems that ice crystals are not the only reason for cell damage. The experiments with different thawing rates indicate that approximately 45° C. per second is optimum. While glycerol in a concentration of 10 per cent was not suitable for preservation of rabbit platelets at the high freezing and thawing rates used, the results with DMAC were encouraging. Toxicity of DMAC for human platelets and for animals and man should be determined. In addition, it should be clarified whether human platelets behave similarly to those of the rabbit frozen in liquid nitrogen without protective agents. If platelets could be frozen without cryophylactic additives, all questions of toxicity both to the platelets and the recipient would be eliminated.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-0584
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The damage of granulocytes by isolation with dextran or ammoniumchloride was studied by evaluating the survival time of DF32P-labeled autologous granulocytes. In five normal persons the granulocytes were concentrated by sedimentation of erythrocytes with dextran. In these cases the half life of granulocytes was significantly reduced with 1,6 hours in comparison with 6,6 hours of a control. In five other cases the granulocytes were concentrated by hemolysing the erythrocytes with ammoniumchloride. There only 2 percent of activity was detectable in peripheral blood three minutes after retransfusion. It is suggested that isolation of granulocytes by hemolysing the erythrocytes with ammoniumchloride cannot be used for transfusion of granulocytes because of severe cell damage. Isolation of granulocytes by sedimentation of erythrocytes with dextran only seems to be of limited value for transfusion.
    Notes: Zusammenfassung Es wurde die Schädigung der Granulozyten durch Isolierung mit Dextran oder NH4Cl an Hand der Bestimmung der Lebensdauer in vivo von DF32P-markierten, autolog transfundierten Granulozyten geprüft. Bei fünf Personen, bei denen die Granulozyten durch Sedimentation der Erythrozyten mit Dextran angereichert worden waren, war die Halbwertszeit der Granulozyten-aktivität mit 1,6 Stunden gegenüber 6,6 Stunden bei der Kontrollgruppe stark verkürzt. Bei fünf Personen, bei denen die Granulozyten durch Hämolyse der Erythrozyten mit NH4Cl angereichert worden waren, waren 3 Min. nach Retransfusion nur noch 2% an Granulozytenaktivität im peripheren Blut nachweisbar. Somit kann die Gewinnung von Granulozyten zu Transfusionszwecken durch Hämolyse der Erythrozyten mit NH4Cl wegen starker Zellschädigung als unbrauchbar, durch Sedimentation der Erythrozyten mit Dextran als nur bedingt geeignet angesehen werden.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Annals of hematology 19 (1969), S. 42-48 
    ISSN: 1432-0584
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-0584
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-1440
    Keywords: Haarzelleukämie ; IFN-Therapie ; Zellmarker ; Thymidineinbau
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung In einer multizentrischen „open label“ Studie mit IFN-α2b wurden 85 Patienten mit Haarzell-Leukämie behandelt und ausgewertet. Die Induktionsbehandlung bestand in 2 × 106 E IFN-α2b/m2, 3 × wöchentlich, s.c. Die Ergebnisse zeigen die hohe Effektivität der Substanz, wobei unter den verwendeten Dosierungen geringe, gut tolerierbare und passagere Nebenwirkungen im Sinne eines grippeähnlichen Syndroms auftraten. Die Prozentzahl der Remissionen (CR + PR + MR) nach sechsmonatiger Behandlungszeit lag bei 89%, der Anteil von CR betrug 4%, von PR 69%. An einer kleinen Gruppe (4 Patienten) wurde nach Erzielen einer CR oder PR der Effekt unterschiedlicher Formen der Erhaltungstherapie getestet. Die bisherigen, statistisch noch nicht auswertbaren Resultate sprechen dafür, daß bei Auftreten eines Rückfalles nach Aussetzen oder Verringerung der IFN-Dosis (3 × 106 I.E., s.c., 1 × wöchentlich) durch Wiedereinsetzen oder Erhöhung von IFN-α2 neuerlich Remissionen erzielt werden können. Zur Erhaltung der Remissionen dürfte eine IFN-Dauertherapie notwendig sein. Mittels eines Kurzzeit in vitro Tests werde der Effekt von Interferon α2 auf die Inkorporation von H3-Thymidin und H3-Uridin in Haarzellen bestimmt. Für beide Präkursoren ergab sich keine Hemmung des Einbaues. Allerdings zeigte eine Langzeitinkubation (48 h) einen signifikant vermehrten H3-Uridin Einbau, während der H3-Thymidin Einbau unbeeinflußt blieb. Diese Resultate sprechen gegen einen unmittelbaren antiproliferativen Effekt von IFN-α2 auf Haarzellen, sie weisen aber auf eine Induktion der RNS-Synthese hin. Eine Zellmarker-Analyse der Haarzellen mit monoklonalen Antikörpern vor und während einer 7tägigen in vitro Behandlung mit IFN-α2 ergab keine Änderung des Phänotyps, so daß eine differenzierende Wirkung des IFN auf Haarzellen nicht beobachtet werden konnte.
    Notes: Summary Eighty-five patients with hairy-cell leukemia were treated in a multicentric “open label” study with IFN-α2b and evaluated. Induction therapy was 2 × 106 U IFN-α2b/m2, 3 times a week, s.c. The results show this regimen to be highly effective with only a few tolerable and transient side effects consisting mainly of flu-like symptoms. After 6 months of therapy 4% CR, 69% PR, and 16% MR, were noted. In a small group of four patients who had achieved CR or PR, we tested the effect of varying doses for maintenance therapy. Our preliminary results indicate that a relapse caused by interruption of IFN therapy or dose reduction to 3 × 106 U given once a week, o.c. could be successfully treated by readministration, or escalating the dosage of IFN. It seems that remission maintenance requires long-term treatment with IFN. In a short-term in vitro test we studied the effect of IFN-α2 on the incorporation of3H-thymidine and3H-uridine into hairy cells of five patients. Fort both precursors no appreciable effect was detected. However, after prolonged incubation for 48 h, a significant enhancement of3H-uridine incorporation was observed, while3H-thymidine incorporation remained unaffected. Cell marker analysis performed with monoclonal antibodies before and after incubation of hairy cells with IFN-α2 for up to 7 days did not reveal any change of the phenotype of hairy cells.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular medicine 47 (1969), S. 1283-1285 
    ISSN: 1432-1440
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The cytotoxicity of antihumanlymphocytic globulin against human monocytes isolated from peripheral blood was investigated. In the presence of complement the ALG preparations tested exhibited a cytotoxic effect against monocytes, which however was much less pronounced than against lymphocytes. By absorbing the sera with lymphocytes the cytotoxic effect against monocytes was also removed. If lymphocytes sensitized with ALG were added to monocytes rosette formation became demonstrable and in the presence of complement was detected up to high ALG dilutions.
    Notes: Zusammenfassung Die Cytotoxizität von Antihumanlymphocytenglobulin auf menschliche Monocyten aus dem peripheren Blut wurde untersucht. Es zeigte sich, daß alle untersuchten Seren gegen Monocyten bei Gegenwart von Komplement cytotoxisch waren, allerdings in viel geringerem Maße als gegenüber Lymphocyten. Der cytotoxische Effekt gegenüber Monocyten konnte durch Absorption mit Lymphocyten entfernt werden. Bei Zusatz von ALG sensibilisierten Lymphocyten zu Monocyten kam es zur Rosettenbildung, die in Gegenwart von Komplement bis zu hohen ALG-Verdünnungen nachweisbar war.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-1440
    Keywords: Lymphocytes ; Subpopulations ; Chronic lymphocytic leukaemia ; Lymphocyten ; Subpopulationen ; Chronische Lymphadenose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Zirkulierende Lymphocyten setzen sich aus funktionell heterogenen Zellen zusammen, welche mit morphologischen Methoden allein nur ungenügend differenziert werden können. Unter Verwendung immunologischer und autoradiographischer Methoden wurden Lymphocyten mit Determinanten leichter (kappa) Ketten, Lymphocyten mit Membranreceptoren für Immun-Komplement-Komplexe, lymphatische Zellen mit cytoplasmatischem Immunglobulingehalt und Lymphocyten in DNS-Synthese bestimmt. Gegenüber Normalpersonen waren bei Patienten mit chronischer Lymphadenose Abweichungen an mehreren dieser Lymphocytenpopulationen nachweisbar. Die Ergebnisse werden im Hinblick auf das Konzept thymus- und knochenmarkabhängiger Lymphocyten diskutiert, deren Differenzierung mit immunologischen Methoden erleichtert wird.
    Notes: Summary Circulating lymphocytes consist of a heterogenous cell population. Employing immunological and autoradiographic methods lymphocytes with Ig (kappa) determinants, those with membrane receptors for immune-complement complexes, lymphocytes containing Ig intracellularly and those in DNA synthesis were evaluated in normals and in patients with chronic lymphocytic leukaemia (CLL). The concept of functionally defective lymphocytes in CLL was further substantiated. The use of immunological markers for identifying thymus (T)- and bone marrow (B) derived human lymphocytes is discussed.
    Type of Medium: Electronic Resource
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