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  • 1
    Electronic Resource
    Electronic Resource
    Interactions of guanine derivatives with ethylenediamine and diethylenetriamine complexes of palladium(II) in solution: Pd binding sites of the guanine ring and formation of a cyclic adduct, [{Pd(en)(guanine ring)}4] (1989)
    s.l. : American Chemical Society
    Inorganic chemistry 28 (1989), S. 2067-2073 
    Details
    ISSN: 1520-510X
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ic00310a012
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  • 2
    Electronic Resource
    Electronic Resource
    Role of cytoplasmic inorganic phosphate in light-induced activation of H+-pumps in the plasma membrane and tonoplast of Chara corallina (1992)
    Springer
    Planta 186 (1992), S. 466-472 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A unique variant strain of Chara corallina, which contains little inorganic phosphate in the vacuole ([Pi]v) was isolated. The level of cytoplasmic inorganic phosphate ([Pi]c) in these cells was the same as that in normal cells. Using these unique cells, we studied the change in [Pi]c and the effect of Pi on the activities of electrogenic H+-pumps associated with the plasma membrane and tonoplast. Upon illumination, the plasma membrane of C. corallina became hyperpolarized by 15 mV, the pH of the vacuolar sap decreased by 0.5 unit, and [Pi]c decreased by 30% with a similar time course. The activities of the electrogenic H +-pump in the plasma membrane and the ATP and PPi-dependent H+-transport in the tonoplast were noncompetitively inhibited by Pi with Ki values of, in the order given, 21.3 mM, 22.1 mM and 37.7 mM. From the kinetics study we calculated that the electrogenic H+-pump in the plasma membrane and the ATP and PPi-dependent H+ transport in the tonoplast were activated by, again in this order, 13%, 13% and 9%, in accordance with the decrease in [Pi]c. We propose that the change in [Pi]c is one of the regulators of photosynthesis-mediated activation of the H+-pumps in the plasma membrane and the tonoplast in C. corallina upon illumination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00195329
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  • 3
    Electronic Resource
    Electronic Resource
    Identification of metabolites of 2-chlorodibenzofuran in the rat (1991)
    Springer
    Archives of environmental contamination and toxicology 20 (1991), S. 67-72 
    Details
    ISSN: 1432-0703
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Notes: Abstract The hepatic metabolism of 2-chlorodibenzofuran was investigated in the rat. When 2-chloro[14C]dibenzofuran was intravenously administered to bile duct-cannulated rats, about 90% of radioactivity was excreted in bile and urine within 6 hr, the bile being the preferential route. Another group of rats received oral administration of cold 2-chlorodibenzofuran and bile fluid was collected by surgical bile duct cannulation for qualitative analysis. Three hydroxylated metabolites were isolated by high performance liquid chromatography from the bile fluid after hydrolytic digestion with sulfatase and β-glucuronidase and were identified by mass and nuclear magnetic resonance spectrometries to be 2-chloro-3, 7-dihydroxydibenzofuran, 2-chloro-3-hydroxydibenzofuran and 2-chloro-7-hydroxydibenzofuran, respectively. Analyses of the radioactive bile fluid by thin layer chromatography revealed that there was no detectable amounts of unmetabolized 2-chlorodibenzofuran in the bile fluid and the hydroxylated metabolites were present not as aglycons but as conjugated substances. The results suggest that 2-chlorodibenzofuran is rapidly metabolized in the rat, and the 3 and/or 7 positions play an important role in the metabolism of 2-chlorodibenzofuran.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01065330
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  • 4
    Electronic Resource
    Electronic Resource
    Changes in sarcoplasmic metabolite concentrations and pH associated with the catch contraction and relaxation of the anterior byssus retractor muscle ofMytilus edulis measured by phosphorus-31 nuclear magnetic resonance (1991)
    Springer
    Journal of muscle research and cell motility 12 (1991), S. 242-246 
    Details
    ISSN: 1573-2657
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The sarcoplasmic concentrations of phosphorus metabolites and pH (pHin) were measured in the anterior byssus retractor muscle (ABRM) ofMytilus edulis by31P nuclear magnetic resonance spectroscopy. During an active contraction induced by 10−3 m acetylcholine, the concentration of arginine phosphate ([Arg-P]in) decreased from the resting value of 7.47±0.26 (mean±se,n=8) to 6.67±0.29 (n=6) μmol g−1, and that of inorganic phosphate (Pi) consistently increased from 0.84±0.06 (n=7) to 1.61±0.12 (n=5) μmol g−1. In the ‘catch’ state following the active contraction, these concentrations were close to their resting levels, indicating that the catch is an inactive state. 5-hydroxytryptamine caused a rapid relaxation of the catch, which was associated with a slight decrease in [Arg-P]in and an increase in pHin by ca 0.2 units. The sarcoplasmic concentration of ATP (mean, 1.6μmol g−1) did not change throughout the contraction-relaxation cycle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01745113
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  • 5
    Electronic Resource
    Electronic Resource
    Phosphorus-31 nuclear magnetic resonance study on the effects of endurance training in rat skeletal muscle (1992)
    Springer
    European journal of applied physiology 65 (1992), S. 197-201 
    Details
    ISSN: 1439-6327
    Keywords: Phosphorus-31 nuclear magnetic resonance ; Muscle metabolism ; Phosphocreatine ; Adenosine 5′-triphosphate ; Endurance training ; Muscle fibre type
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary To evaluate changes in muscle energetics following endurance training, we measured phosphorus-31 nuclear magnetic resonance (31P NMR) spectra on rat muscle in vivo before and after training in the same animals. The endurance training lasted for 3 months. The31P NMR spectra were obtained serially at rest, during exercise by electrical stimulation, and during recovery. Intramuscular phosphocreatine (PCr), inorganic phosphate (Pi, adenosine 5′-triphosphate (ATP) and pH were determined from the NMR spectra. The ratio of PCr : (PCr + Pi) at rest showed no difference between the trained and control groups even after 3 months of training. During exercise, however, this ratio was significantly higher in the trained group than in the control group. The ratio also recovered more rapidly after exercise in the trained group. The intramuscular pH decreased slightly by approximately 0.1 pH unit during exercise but did not show a significant difference between the groups. These results indicated that endurance training of 3 months duration improved the ATP supply system in the muscle. They also demonstrated that31P NMR is a potent method for evaluating the effects of training in the same individuals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00705081
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  • 6
    Electronic Resource
    Electronic Resource
    31P nuclear magnetic resonance study on changes in phosphocreatine and the intracellular pH in rat skeletal muscle during exercise at various inspired oxygen contents (1996)
    Springer
    European journal of applied physiology 74 (1996), S. 305-310 
    Details
    ISSN: 1439-6327
    Keywords: 31P nuclear magnetic resonance ; Hypoxic exercise ; Phosphocreatine ; Intracellular pH Oxygen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We measured ATP, phosphocreatine (PCr), inorganic phosphate (Pi), and the intracellular pH in rat hindlimb muscles during submaximal isometric exercise with various O2 deliveries using31P nuclear magnetic resonance spectroscopy (31P NMR) to evaluate changes in energy metabolism in relation to O2 availability. Delivery of O2 to muscles was altered by controlling the fractional concentration of inspired oxygen (F IO2) at 0.50, 0.28, 0.21, 0.11 and 0.08 with monitoring partial pressure of oxygen and carbon dioxide, and bicarbonate at the femoral artery. The steady-state ratio of PCr : (PCr + Pi) during exercise decreased as a function ofF IO2 even at 0.21. Significant acidification of the intracellular pH during exercise occurred at 0.08F IO2. Change in the PCr : (PCr + Pi) ratio demonstrated that the oxidative capacity, i.e. the maximal rate of the oxidative phosphorylation reaction, in muscle was not limited by O2 delivery at 0.50F IO2, but was significantly limited at 0.21F IO2 or below. Change in the intracellular pH at 0.08F IO2 could be interpreted as an increase in lactate, suggesting activation of glycolysis. Correlation between the PCr : (PCr + Pi) ratio and the intracellular pH revealed the existence of a critical PCr : (PCr + Pi) ratio and pH for glycolysis activation at around 0.4 and 6.7, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02226925
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