Library

feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The gene responsible for multiple endocrine neoplasia type 2A (MEN2A) has recently been assigned to the pericentromeric region of chromsome 10 in European Caucasian kindreds by linkage analysis using a DNA marker, interstitial retinol-binding protein 3 (RBP3). We have found tight linkage between the MEN2A and RBP3 loci in Japanese MEN2A kindreds. The maximum lod score is 5.19 at a recombination fraction of 0.00. This result suggests that mutation of a certain gene close to RBP3 is responsible for MEN2A irrespective of ethnic backgrounds.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 2
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Multiple endocrine neoplasia type 2A (MEN2A) is a dominantly inherited cancer syndrome characterized by medullary thyroid carcinoma, pheochromocytoma, and parathyroid hyperplasia. The gene responsible for MEN2A was localized by linkage analysis to chromosome 10q11.2 in 1987, and recently mutations in RET, a proto-oncogene in the candidate region, were discovered in patients with MEN. The majority of mutations found so far in MEN2A patients have been located in nucleotide sequences encoding cysteine residues in the extracellular domain of RET. To characterize MEN2A germline alterations in the Japanese population, we screened DNA from eight unrelated patients for mutations in exons 10 and 11 of the RET proto-oncogene and found mutations in all eight patients, at codons 618, 620, or 634; each of these sites encodes a cysteine residue in the extracellular domain of RET. The mutations were confirmed in other affected individuals in the respective families by digestion of polymerase chain reaction (PCR) products containing the mutated codons with restriction enzymes (RsaI, CfoI, or AluI) for which cleavage sites had been generated by the specific genetic alteration. These PCR-restriction enzyme systems will be useful for genetic diagnosis in members of families carrying these mutations.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 3
    ISSN: 1432-2307
    Keywords: Thyroid medullary carcinoma ; Calcitonin ; EC granule ; Serotonin ; Protein A-colloidal gold
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In two medullary carcinomas of the thyroid gland two types of secretory granules were found electron microscopically in the cytoplasm of the tumour cells. The sizes of the granules in one case ranged 103–345 nm in diameter; they were round in shape, and they co-existed in the same tumour cell. They could not, therefore, be distinctively subdivided into two types. In another case, secretory granules in the cytoplasm closely resemble EC granule in morphology. Using the protein A-colloidal gold (PAG) technique the content of secretory granules could be identified as calcitonin irrespective of their sizes or morphology. Immunoreactivity at the ultrastructural level was fairly well preserved even in the osmium-fixed tumour cells. The labelling index, expressed as a mean number of gold particles per unit square area of the secretory granule, was higher in the non-osmium-fixed tumour cells than in the osmium-fixed. Non-osmium-fixed tumour cells embedded either in epoxy or methacryl resin were almost equally labelled with gold particles. The result indicates that the PAG method is practicable to demonstrate the ultrastructural localization of calcitonin even in the osmium-fixed, epoxy resin embedded material.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 4
    ISSN: 1432-2323
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Dix malades présentant un cancer médullaire thyroïdien et 2 malades un cancer papillaire ont été soumis à une scintigraphie employant un nouveau radioisotope le99mTc(V)-DMS. Au cours de l'intervention une dose traçante de ce nouvel agent fut injectée par voie intraveineuse pour permettre l'étude de sa répartition au niveau de la pièce opératiore. Parmi les malades atteints de cancer médullaire 7 sur 10 ont présenté des images scintigraphiques patentes, 2 des images estompées cependant qu'un malade qui était porteur d'une récidive au niveau d'un ganglion lymphatique ne présentait pas d'image significative. Chez 3 malades une diffusion médiastinale fut clairement démontrée et la dissection médiastinale vint confirmer les constatations scintigraphiques. En revanche aucune image ne fut observée chez les malades atteints de cancer papillaire. Les études de la distribution tissulaire du99mTC(V)-DMS démontrent une imprégnation spécifique importante du néoplasme médullaire par rapport aux autres tissus. Ce nouvel agent scintigraphique de dépistage du cancer médullaire thyroïdien est d'une grande valeur pour déterminer la conduite opératoire et pour suivre le malade après l'intervention.
    Abstract: Resumen Diez pacientes con carcinoma medular de tiroides (CMT) y 2 pacientes con carcinoma papilar fueron investigados mediante centelleografía utilizando99mTc(V)-ácido dimercaptosuccínico, un nuevo agente radiofarmacéutico para imágenes de CMT. En el curso de la cirugía se administró una dosis trazadora del agente por vía intravenosa, y su distribución fue estudiada en los tejidos de la resección quirúrgica. Entre los pacientes con CMT, 7 presentaron imágenes claras de centelleografía del tumor, 2 presentaron imágenes débiles, y 1 paciente con tumor recurrente en un ganglio linfático no exhibió imagen de significación. En 3 pacientes la invasión mediastinal pudo ser claramente demostrada, y la disección mediastinal confirmó los hallazgos de la centelleografía. No se obtuvieron imágenes significativas en los pacientes con carcinoma papilar. Los estudios de distribución tisular del99mTc(V)-ADMS revelaron acumulación específica del agente en tejido de CMT y baja captación por otros tejidos. Este nuevo método de centelleografía para CMT es de gran valor para decidir sobre el aproche quirúrgico y para el seguimiento.
    Notes: Abstract Ten patients with medullary thyroid carcinoma (MTC) and 2 patients with papillary carcinoma of the thyroid were investigated by scintigraphy using99mTc(V)-dimercaptosuccinic acid [99mTc(V)-DMS], a new radiopharmaceutical agent for imaging MTC. At surgery, a tracer dose of the agent was administered intravenously, and the distribution of the agent was studied in the surgically removed tissues. Among the patients with MTC, 7 had clear scintigraphic images of tumors, 2 had faint images, and 1 patient with a recurrent tumor in a lymph node had no significant image. In 3 patients, mediastinal involvements were clearly demonstrated, and mediastinal dissection confirmed the scintigraphic findings. No significant images were obtained in the patients with papillary carcinoma. The tissue distribution studies of99mTc(V)-DMS revealed specific accumulation of the agent in MTC tissue and low uptake in other tissues. This new scintigraphy for MTC is of great value in deciding the surgical approach and follow-up.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 5
    Electronic Resource
    Electronic Resource
    Springer
    World journal of surgery 12 (1988), S. 591-592 
    ISSN: 1432-2323
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 6
    Electronic Resource
    Electronic Resource
    Springer
    World journal of surgery 16 (1992), S. 688-689 
    ISSN: 1432-2323
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 7
    ISSN: 1432-2323
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Thyroid lymphoma occurs most commonly in the thyroid gland in association with Hashimoto’s thyroiditis. Histologic findings occasionally cannot distinguish lymphoma from Hashimoto’s thyroiditis, which creates a serious problem of whether treatment should be initiated. For this study, we examined 33 lymphoma tissues and 10 thyroid tissues from patients with Hashimoto’s thyroiditis for the presence of gene rearrangement of immunoglobulin, which represents clonality of B-cell-derived tumors. Genomic DNA from thyroid tissues was digested with Bam H1 and Hind III restriction enzymes followed by electrophoresis. A Southern blot was performed with an IgH-JH probe or IgL-Jκ probe to detect gene rearrangement. Of the 33 lymphoma tissues, 27 (85%) showed gene rearrangement of immunoglobulin, whereas none of Hashimoto’s thyroiditis tissue showed gene rearrangement. Five patients with a positive histologic diagnosis of lymphoma showed a negative gene rearrangement and were treated as having lymphoma. We encountered one case of lymphoma (plasmacytoma) in which gene rearrangement (not histologic findings) was diagnostic. Gene rearrangement of immunoglobulin can be used to detect thyroid lymphoma, particularly when the histologic diagnosis is inconclusive. The sensitivity of detecting thyroid lymphoma by the Southern blot method was about 85% in the present series.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 8
    ISSN: 1432-2307
    Keywords: Thyroid neoplasms ; Ultrastructure prognosis ; C cell ; Calcitonin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Five cases of sporadic medullary carcinoma of the thyroid (MTC) with rapidly progressive disease were studied ultrastructurally. The tumour cells had poorly differentiated C cell characteristics. They exhibited smaller secretory granules in their narrow cytoplasm. Morphometric analysis disclosed that the average diameter of the secretory granules of the cases with a poor prognosis was 173.0 nm in comparison with 254.2 nm of well differentiated cases. The granules were fewer in the poor prognostic group (1.31/µ2) than the well differentiated group (2.75/µ2). Increased free ribosomes and polysomes were noted in the cytoplasm and dispersed chromatin in the nuclei. These cases should be therefore classified as poorly differentiated MTC rather than atypical or anaplastic MTC.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 9
    ISSN: 1432-2323
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Le taux de calcitonine sérique avant (S1) et après (S2) l'acte chirurgical ont été mesurés chez 67 patients ayant un cancer médullaire de la thyroïde; on a ensuite mesuré les taux de calcotonine de façon répétée pendant 7 ans en moyenne. Le temps de doublement de calcitonine a été calculé chez les patients ayant des taux élevés de calcitonine en postopératoire. En assumant que le taux de calcitonine augmente proportionnellement au poids tumoral, le poids tumoral résiduel (W2=W1S2/(S1−S2) où W1=poids de la tumeur réséquée. L'index de réduction (α) est défini par W2/(W1+W2)=(1/2) puissanceα. Le produitαT2 indique la prolongation de survie procurée par l'acte chirurgical (PSP). L'index de survie (β) se définit par le nombre de doublements de la tumeur résiduelle jusqu'à ce qu'elle pèse 1,000 g, ce qui provoque en général la mort du patient. La durée espérée de survie après chirurgie (DES)=βT2. La survenue de mort avant 3 ans, ou de récidive avant 5 ans était corrélée au mieux avec une PSP courte, suivie d'un T2 court ou d'unα petit. S2 était peu corrélé et S pratiquement pas corrélé du tout avec le pronostic. La durée de survie chez 3 patients morts de leur maladie était très près de la DES. T2 indique le taux de croissance de la tumeur,α indique le degré de radicalité de la chirurgie, PSP indique les effets d la chirurgie, et DES la durée de survie après chirurgie. Ces paramètres permettent d'évaluer quantitativement les effets de la chirurgie et d'établir le pronostic de chaque patient.
    Abstract: Resumen Los niveles de calcitonina sérica fueron medidos antes (S1 y después (S2) de la cirugía en 67 pacientes con carcinoma medular de tiroides y los pacientes fueron seguidos con mediciones seriadas de calcitonina durante un promedio de 7 anos. El tiempo de doblaje de la calcitonina fue calculado en los pacientes con niveles postoperatorios elevados de calcitonina. Bajo la presunción de que el nivel sérico de calcitonina se correlaciona en forma linear con el peso del tumor, el peso residual del tumor (W2) puede ser estimado como W1S2/(S1−S2), donde W1 es el peso del tumor resecado. El índice de reducción (α) es definido como W2/(W1+W2)=(1/2)α.αT2 indica la prolongación esperada de la supervivencia (PES) por la cirugía. El indice de supervivencia (β) es definido como el número de doblajes del tumor residual hasta un peso de 1,000 g, el cual generalmente mata al paciente. La duratión esperada de la supervivencia (DES) después de la cirugía es definida comoβT2. La muerte dentro de los 3 años despues de la cirugía o la recurrencia dentro de los 5 anos apareció más íntimamente asociada con una corta PES, seguida de una corta T2 o pequeñoα. S2 presentó una correlación débil, y S1 casi ninguna, con el pronóstico. La duración de la supervivencia en 3 pacientes que murieron de la enfermedad apareció muy cercana a la DES. T2 indica la tasa de crecimiento del tumor,α indica el grado de radicalidad de la cirugía, PES indica los efectos de la cirugía, y DES indica la duración de la supervivencia después de la cirugía. Estos parámetros permiten una apreciación cuantitativa del efecto de la cirugía y una predicción cuantitativa del pronóstico en cada paciente.
    Notes: Abstract Serum calcitonin levels before (S1) and after (S2) surgery were measured in 67 patients with medullary thyroid carcinoma and the patients were followed with serial calcitonin measurements for a mean duration of 7 years. The calcitonin doubling time (T2) was calculated in patients with elevated postoperative calcitonin levels. Assuming that the serum calcitonin level is linearly correlated with tumor weight, the residual tumor weight (W2) is estimated as W1S2/(S1−S2) where W1 is the weight of the resected tumor. The reduction index (α) is defined as W2/(W1+W2)=(1/2)α.α T 2 indicates the expected prolongation in survival (EPS) by surgery. The survival index (β)is defined as the number of doublings of residual tumor until it weighs 1,000 g, which would generally kill the host. The expected duration of survival (EDS) after surgery is estimated as β T 2.Death within 3 years after surgery or recurrence within 5 years was best associated with short EPS followed by short T 2 or smallα.S 2 had a rather weak correlation and S 1 had almost no correlation with the prognosis. The duration of survival in 3 patients who died of the disease was very close to the EDS. T 2 indicates growth rate of the tumor,α indicates degree of radicality of the surgery, EPS indicates the effects of the surgery, and EDS indicates duration of survival after surgery. These parameters allow quantitative judgment of the effect of surgery and quantitative prediction of the prognosis in each patient.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 10
    ISSN: 1432-2323
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé L'évolution à long terme des nodules thyroïdiens solitaires palpables a été étudiée en surveillant 441 patients opérés et 140 patients ayant un nodule non traité pendant une moyenne de 15+/-4.5 ans. Dans notre étude clinique, le cas le plus fréquent était la disparition du nodule (38.3%). De Même, 36.3% des patients ont eu une intervention chirurgicale dans un autre éstablissement. Cinq patients sont morts d'un cancer de la thyroïde (1.1%). Lorsque les nodules ont été réexaminés, la plupart étaient soit plus petits, soit non retrouvés. En revanche, 13% des nodules ont augmenté de volume. L'examen échographique a montré que la plupart des nodules dits solitaires étaient en fait multiples ou associés à des kystes. La ponction à l'aiguille fine des nodules retrouvait 26.3% de cancer pour les nodules qui avavient augmenté de volume, contre 6.4% pour ceux n'ayant pas changé de taille. Le risque de développer un cancer décroît lorsque la taille du nodule diminue. Quinze patients ayant un nodule suspect ont été opérés. Les interventions ont consisté en une lobectomie, une thyroïdectomie subtotale, ou une résection des nodules, associée ou non à un curage ganglionnaire. Sept patients avaient un cancer papillaire. Deux patients avaient un microfoyer de cancer papillaire alors que la cytologie par voie percutanée était rassurante. Dans notre étude, la plupart des nodules thyroïdiens solitaires palpables avaient une tendance à diminuer de taille; ces nodules ne nécessitent pas d'autre traitement. Un nodule qui augmente de volume présente un risque de dégénérescence. Si la taille du nodule reste stable, une ponction à l'aiguille est indiquée.
    Abstract: Resumen Nos propusimos investigar el resultado final de la evolución a largo plazo de los nódulos tiroideos solitarios, mediante un cuestionario enviado a 441 pacientes y el examen de 140 que poseían nódulos tiroideos no tratados con una evolución de 15±4.5 (DE) años. En nuestro cuestionario clínico el hallazgo más común fue la desaparición del nódulo (38.3%). También se encontró que un número significativo de pacientes (36.3%) fueron sometidos a cirugía en otros hospitales. Cinco pacientes (1.1%) murieron de cácner tiroideo. Cuando los nódulos fueron reexaminados, la mayoría realmente habían disminuido de tamaño o había desaparecido; sin embargo, 13% habían aumentado de tamaño. La ultrasonografía demostró que la mayoría de los nódulos solitarios eran múltiples y quístico-sólidos. Se apreció una tasa mayor de calcificación en los nódulos de larga evolución. Por medio de la aspiración con aguja fina se encontró cáncer en 26.3% de los nódulos que exhibían crecimiento, y 6.4% en los que no registraban cambio en su tamaño. El riesgo de cáncer fue menor en los nódulos que mostraron disminución de tamaño. Un total de 15 pacientes con sospecha de malignidad fue sometido a cirugía; la operación consistió en lobectomía, tiroidectomía casi total o resección de los nódulos con o sin disección cervical modificada. Siete pacientes tuvieron carcinoma papilar y dos, con citología benigna, tenían carcinoma micropapilar. En nuestro estudio se encontró que la mayoría de los nódulos solitarios palpables tendió a disminuir de tamaño; tales nódulos no requieren tratamiento. Nódulos sólidos que aumentan de tamaño definitivamente tienen riesgo de cáncer tiroideo. En los nódulos de tamaño estacionario esta indicado un juicioso aproche fundamentado en la aspiración con aguja fina.
    Notes: Abstract We investigated the outcome of long standing palpable solitary thyroid nodules by surveying 441 patients and examining 140 patients who had untreated thyroid nodules for 15±4.5 years. In our clinical survey, the most common outcome was disappearance of the thyroid nodule (38.3%). Also a significant number of patients (36.3%) underwent surgery in other hospitals. Five (1.1%) patients died of thyroid cancer. When thyroid nodules were re-examined, most nodules indeed decreased in size or disappeared; however, 13% of nodules increased in size. Ultrasound of the nodules showed that most solitary nodules were multiple and partially cystic and solid. There was an increased incidence of calcification in long standing nodules. Thyroid cancer was found by fine needle aspiration biopsy in 26.3% of enlarging nodules and 6.4% of nodules without changing size. The risk of cancer decreased when the size of the nodule decreased. A total of 15 patients with suspicion of malignancy underwent surgery. Surgical procedures were lobectomy, near total thyroidectomy, or resection of nodules with or without modified neck dissection. Seven patients had papillary carcinoma and 2 patients with benign cytology had microscopically evident papillary carcinoma. In our study, the majority of palpable solitary thyroid nodules tended to decrease in size; these nodules do not require treatment. Enlarging solid nodules are a definite risk for thyroid cancer. If the size of the nodule remains the same, judicious approach with fine needle aspiration biopsy is needed.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...