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  • 1
    Electronic Resource
    Electronic Resource
    Oesophageal squamous cell carcinoma with lymphoid stroma (1989)
    Springer
    Virchows Archiv 415 (1989), S. 473-479 
    Details
    ISSN: 1432-2307
    Keywords: Oesophageal carcinoma ; Squamous cell carcinoma ; Lymphoid stroma ; S-100 Protein positive cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We treated a 70-year-old Japanese man with squamous cell carcinoma of the oesophagus with evidence of lymphoid stroma. The tumour consisted of a main lesion invading the muscular layer of the oesophagus, in association with wide areas of carcinoma in situ. The tumour stroma of the lesion was nondesmoplastic and was uniformly infiltrated mainly by abundant lymphocytes and plasma cells. Immunohistochemically, the lymphocytes consisted of a large number of T lymphocytes and a small number of B lymphocytes. S-100 protein positive cells were marked in the tumour cell nests and necrotic change of tumour cells was frequent. Abundant infiltration of lymphocytes and plasma cells was also wide-spread beneath the carcinoma in situ, together with the lymphoid follicles. Carcinoma with lymphoid stroma can occur not only in the breast, uterine cervix, nasopharynx and stomach but also in the oesophagus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00747749
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  • 2
    Electronic Resource
    Electronic Resource
    Meeting report of the 72nd Japanese Gastric Cancer Congress (2000)
    Springer
    Gastric cancer 3 (2000), S. 1-8 
    Details
    ISSN: 1436-3305
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00011683
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  • 3
    Electronic Resource
    Electronic Resource
    Increased cytotoxicity of low-dose, long-duration exposure to 5-fluorouracil of V-79 cells with hyperthermia (1991)
    Springer
    Cancer chemotherapy and pharmacology 28 (1991), S. 251-254 
    Details
    ISSN: 1432-0843
    Keywords: Hyperthermia ; 5-Fluorouracil ; Timing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We examined the cytotoxic effects of combined low dose and long exposure to 5-fluorouracil (5-FU) and hyperthermia on Chinese hamster V-79 cells with reference to timing and sequence of administration. The survival rate following hyperthermia at 42°C for 2 h alone was 95.4%, and that after exposure to 1.0 μg/ml/5-FU alone for 48 hours, 94.2%. With respect to the combination of 5-FU and heat, the survival rate of cells exposed to hyperthermia at 42°C for 2 h followed by 1.0 μg/ml 5-FU treatment for 48 h followed by hyperthermia led to a survival rate of 10%. Flow cytometric analysis of V-79 cells after exposure to 1.0 μg/ml 5-FU for 48h revealed an accumulation of cells in the S-phase; the percentage of S-phase exponential growing cells was 65% and the plateau phase was 38%. The former were more sensitive to heat than the latter cells according to the MTT assay. V-79 cells pretreated with 5-FU were more sensitive to hyperthermia than were those not pretreated with 5-FU. Therefore, when 5-FU plus heat is to be used to treat a patient with a malignancy, the sequence of 5-FU followed by hyperthermia may be more effective than the reverse.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00685530
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  • 4
    Electronic Resource
    Electronic Resource
    Quantitative analysis of cytokine mRNA expression in peripheral blood mononuclear cells following treatment with interleukin-2 (1997)
    Springer
    Cancer immunology immunotherapy 44 (1997), S. 329-334 
    Details
    ISSN: 1432-0851
    Keywords: Key words Cytokine ; Quantitative RT-PCR ; Interleukin-2 ; Interleukin-1 ; Tumor necrosis factor α
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  After activation with interleukin-2 (IL-2), peripheral blood mononuclear cells (PBMC) have been reported to induce the expression of mRNA coding various cytokines, including interleukin(IL)-1α, -1β and tumor necrosis factor α (TNFα). We examined the cytokine mRNA expression of PBMC following treatment with IL-2 in vitro and in vivo by a quantitative method using the reverse transcription/polymerase chain reaction (RT-PCR). After stimulating PBMC with IL-2 in vitro, peak levels of IL-1α mRNA were reached between 3 h and 12 h, and thereafter declined. The IL-1β expression increased, with levels peaking at 1–6 h and, had decreased by 96 h. The expression of TNFα was elevated both 1–3 h and 24–48 h after stimulation. The peak levels of IL-1α and -1β mRNA and the early elevation of TNFα mRNA mainly accounted for the cytokine mRNA expression in adherent cells; however, the late induction of TNFα mRNA was observed in nonadherent cells. In patients with advanced carcinoma, the IL-1α and -1β mRNA expression were elevated after IL-2 treatment for 5 consecutive days, while the expression of TNFα mRNA also increased. These results indicate that the quantitative RT-PCR method appears to be useful for analyzing the cytokine mRNA expression of PBMC after treatment with IL-2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002620050390
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  • 5
    Electronic Resource
    Electronic Resource
    The expression of costimulatory molecules CD80 and CD86 in human carcinoma cell lines: its regulation by interferon γ and interleukin-10 (1996)
    Springer
    Cancer immunology immunotherapy 43 (1996), S. 213-219 
    Details
    ISSN: 1432-0851
    Keywords: Key words CD80 ; CD86 ; Human carcinoma cell line ; mRNA ; Cytokine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The costimulatory signal through CD80 or CD86 to its counterreceptor CD28 or CTLA-4 on T cells has been shown to play an important role in the induction of T-cell-mediated immunity against tumors. In the present study, we examined the expression of CD80 and CD86 in the cell lines derived from human gastric, esophageal and colorectal carcinomas at the mRNA level, by means of a reverse transcriptase/polymerase chain reaction analysis and, for their surface expression, using a flow-cytometric analysis with monoclonal antibodies (mAb). The expression of mRNA for CD80 or CD86 was detected in all 18 cell lines tested, except for CD86 on one cell line. The cells from 13 (72%) or 12 (67%) of these cell lines expressed the surface CD80 or CD86 molecule, detected with the respective mAb. The surface expression of CD80 or CD86 was increased in four to five of the six cell lines tested after a culture with interferon γ (IFNγ). In addition, the up-regulation of CD80 or CD86 expression by IFNγ was inhibited by interleukin-10 (IL-10). These results indicated that, in the cell lines derived from human gastrointestinal carcinoma, both the costimulatory molecules CD80 and CD86 were detectable in the majority of the cell lines examined at the mRNA and protein levels, and could be regulated with the cytokine IFNγ or IL-10.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002620050324
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  • 6
    Electronic Resource
    Electronic Resource
    Apoptosis in antibody-dependent monocyte-mediated cytotoxicity with monoclonal antibody 17-1A against human colorectal carcinoma cells: enhancement with interferon γ (1996)
    Springer
    Cancer immunology immunotherapy 43 (1996), S. 220-225 
    Details
    ISSN: 1432-0851
    Keywords: Key words Apoptosis ; Antibody-dependent cell-mediated cytotoxicity ; Monocyte ; 17-1A ; Interferon γ
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Antibody-dependent cell-mediated cytotoxicity (ADCC) has been considered to be one of the main effector mechanisms by which unconjugated monoclonal antibody (mAb) 17-1A can exert an antitumor effect in vivo. Since the apoptotic pathway as well as the necrotic pathway have been shown to be utilized in various cytotoxic effector mechanisms, we investigated the role of apoptosis in ADCC mediated by monocytes (ADMC) using mAb 17-1A as an antibody and the human colorectal carcinoma cell line, COLO205, as target cells in vitro. The implications of the apoptosis during ADMC was demonstrated by means of both a DNA fragmentation assay and a TdT-mediated dUTP-biotin nick end labeling (TUNEL) assay. Furthermore, interferon γ (IFNγ) was also found to enhance the induction of apoptosis significantly. The addition of superoxide dismutase did not reduce the level of the apoptosis, although superoxide anion (O2 –) was observed to be produced. However, the release of tumor necrosis factor α (TNFα) was significantly enhanced during ADMC, while, in addition, apoptosis was significantly inhibited by the addition of anti-TNFα antibody. These findings indicated that apoptosis might be implicated in ADMC with mAb 17-1A, which was augmented by IFNγ, while, in addition, TNFα may also be one of the major mediators of apoptosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002620050325
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  • 7
    Electronic Resource
    Electronic Resource
    Generation of specific antitumor reactivity by the stimulation of spleen cells from gastric cancer patients with MAGE-3 synthetic peptide (1999)
    Springer
    Cancer immunology immunotherapy 48 (1999), S. 189-194 
    Details
    ISSN: 1432-0851
    Keywords: Key words Cytotoxic T lymphocyte ; MAGE ; Antigenic peptide ; Spleen cell ; Cancer patient
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The induction of cytotoxic T lymphocytes (CTL) from peripheral blood mononuclear cells (PBMC) using MAGE peptide has been investigated in order to use MAGE antigens immunotherapeutically. We therefore developed a simplified method for inducing peptide-specific CTL that kill tumor cells expressing MAGE from the PBMC of either healthy donors or even cancer patients. Since the spleen is a major lymphoid organ, we used a simple method to examine the capacity of spleen cells to generate MAGE-specific CTL by in vitro stimulation with MAGE peptide in gastric cancer patients. The CTL responses could thus be induced from unseparated spleen cells in HLA-A2 patients with gastric carcinoma expressing MAGE-3 by stimulating these cells with autologous spleen cells pulsed with HLA-A2-restricted MAGE-3 peptide as antigen-presenting cells and by using keyhole limpet hemocyanin and interleukin-7 for the primary culture. The induced CTL were thus able to lyse HLA-A2-positive carcinoma cells transfected with MAGE-3 and expressing MAGE-3, as well as the target cells pulsed with the peptide, in an HLA-class-I or -A2-restricted manner. Since MAGE-specific CTL could be induced from the spleen cells of gastric cancer patients, the spleen appears to play an important role in either clinical tumor vaccination or the treatment of cancer patients by adoptive immunotherapeutic approaches using the MAGE peptide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002620050564
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  • 8
    Electronic Resource
    Electronic Resource
    Efficient induction of antitumor cytoxic T lymphocytes from a healthy donor using HLA-A2-restricted MAGE-3 peptide in vitro (1997)
    Springer
    Cancer immunology immunotherapy 44 (1997), S. 21-26 
    Details
    ISSN: 1432-0851
    Keywords: Key words MAGE-3 ; Peptide ; Cytotoxic T lymphocytes Tumor-rejection antigen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The antigenic peptides encoded by tumor-rejection antigen genes, MAGE-1 and -3, have been identified, and various methods have been utilized for the in vitro induction of MAGE-specific, cytotoxic T lymphocytes (CTL) from peripheral blood mononuclear cells (PBMC) using synthetic peptides. However, all of these methods are technically demanding and thus have a relatively limited usefulness. We herein report a simple and efficient method for the in vitro induction of specific CTL by using the HLA-A2-restricted MAGE-3 peptide from the PBMC of a healthy donor. CTL responses could thus be efficiently induced from unseparated PBMC by stimulation with freshly isolated, peptide-pulsed PBMC as antigen-presenting cells and by using interleukin-7 and keyhole limpet hemocyanin for the primary culture. The induced CTL could thus recognize and lyse not only HLA-A2 target cells pulsed with the peptide but also HLA-A2 tumor cells expressing MAGE-3, in an HLA-class-I-restricted manner. This simple method may, therefore, become a useful tool for investigating the potential peptides for tumor antigens as well as for developing various immunotherapeutic approaches for human malignant tumors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002620050350
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  • 9
    Electronic Resource
    Electronic Resource
    p27 expression and gastric carcinoma (1997)
    [s.l.] : Nature Publishing Group
    Nature medicine 3 (1997), S. 593-593 
    Details
    ISSN: 1546-170X
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] To the editor — A cyclin-dependent kinase inhibitor, p27rapl, regulates progression from Gl into S phase. We read with great interest that the decrease or absence of p27 protein expression is a powerful negative prognostic marker in patients with breast1 and colorectal carcinomas2 and is a ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nm0697-593
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  • 10
    Electronic Resource
    Electronic Resource
    A functional SNP in CILP , encoding cartilage intermediate layer protein, is associated with susceptibility to lumbar disc disease (2005)
    [s.l.] : Nature Publishing Group
    Nature genetics 37 (2005), S. 607-612 
    Details
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Lumbar disc disease (LDD) is caused by degeneration of intervertebral discs of the lumbar spine. One of the most common musculoskeletal disorders, LDD has strong genetic determinants. Using a case-control association study, we identified a functional SNP (1184T → C, resulting ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/ng1557
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