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  • 1
    Electronic Resource
    Electronic Resource
    Immunohistochemical demonstration of carbonic anhydrase III and muscle-specific enolase in paraffin-embedded human skeletal muscle sections (1985)
    Springer
    Acta neuropathologica 68 (1985), S. 74-76 
    Details
    ISSN: 1432-0533
    Keywords: Muscle ; Carbonic anhydrase ; Enolase (phosphopyruvate hydratase) ; Paraffin ; Histocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We have demonstrated the histochemical fiber types of human skeletal muscle in paraffin sections by the immunohistochemical method, together with compatible observations previously made in frozen sections that carbonic anhydrase III is mainly localized in type 1 fibers (Shima et al. 1983), and muscle-specific enolase in type 2 fibers (Ibi et al. 1983). This method is useful to analyze the fiber types when frozen muscle samples at biopsy or autopsy cannot be obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00688959
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  • 2
    Electronic Resource
    Electronic Resource
    Permeabilization of yeast cells: Application to study on the regulation of AMP deaminase activity in situ
    Amsterdam : Elsevier
    Analytical Biochemistry 105 (1980), S. 407-413 
    Details
    ISSN: 0003-2697
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0003-2697(80)90479-0
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  • 3
    Electronic Resource
    Electronic Resource
    Cytokeratin immunolocalization and lectin binding studies in oesophageal squamous dysplasia (1996)
    Oxford, U.K. and Cambridge, USA : Blackwell Science Ltd
    Histopathology 29 (1996), S. 0 
    Details
    ISSN: 1365-2559
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: We examined full thickness specimens of oesophageal squamous dysplasia from both cancer-free and cancer patients using immunohistochemical labelling for cytokeratin subtypes 10/13 and 14 and for involucrin, binding studies for various lectins, and PAS/D staining before and after diastase treatment. We studied specimens from patients with oesophageal carcinoma (52 normal epithelia, and 49 with mild, 38 with moderate, and 32 with severe dysplasia), and 32 specimens from cancer-free patients (five normal epithelia and 16 with mild and 11 with moderate dysplasia). Abnormal cytokeratin expression patterns in atypical cells, i.e. both cytokeratin 10/13 and cytokeratin 14 immunoreactivity in the same cells was detected in 41 of 99 specimens with dysplasias in cancer patients. Helix aspersaErythrina cristagalli and Robinia pseudoacacia binding was consistently negative in atypical cells in squamous dysplasia. The non-atypical layer of squamous dysplasia, which was morphologically indistinguishable from the corresponding layer of normal oesophageal squamous epithelium, showed abnormal involucrin expression in 39/101 specimens, Helix aspersa binding in 74/106, diastase sensitive PAS staining in 52/110, Erythrina cristaglli binding in 28/107, and Robinia pseudoacacia binding in 16/100. There were no significant differences in the expression of these markers in dysplasia between cancer patients and cancer-free individuals with the exception of increased Robinia pseudoacacia binding in the non-atypical layer in cancer-free patients. The results indicate that abnormal patterns of cytokeratin expression and lectin binding occur not only in atypical cells but also in non-atypical cells in oesophageal squamous dysplasia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2559.1996.d01-480.x
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  • 4
    Electronic Resource
    Electronic Resource
    Differential expression of cornified cell envelope precursors in normal skin, intraorally transplanted skin and normal oral mucosa (2003)
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 148 (2003), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary Background Skin flaps have routinely been used as substitutes for oral mucosa after extensive resection of oral tissues. However, it remains unknown how the transplanted skin flaps perform as a host defence in the new environment of the oral cavity. Objectives To evaluate the expression of cornified cell envelope (CCE) precursors in pretransplanted (normal) skin, intraorally transplanted skin and normal oral mucosa, because CCEs are highly responsible for a protective barrier in each type of epithelium. Methods We used immunohistochemistry and immunoelectron microscopy to examine the expression of CCE precursors, small proline-rich protein (SPR) 2 and 3 and loricrin, in biopsy specimens of normal skin, transplanted skin and normal oral mucosa, including buccal and lingual (non-keratinized) mucosae, and palatal (keratinized) mucosa. Results Transplanted skin flaps were classified into two groups. About two-thirds of the transplanted skin flaps displayed a reddish appearance and were devoid of the stratum corneum (SC) together with a psoriasiform inflammatory tissue reaction. Others showed a native appearance, retaining the SC. While SPR2 expression was limited to the stratum granulosum (SG) in both normal and transplanted skin retaining the SC, it extended to the stratum spinosum (SS) of the transplanted skin lacking the SC and that of the normal oral mucosa. Although SPR3 expression was not found in normal skin or in the transplanted skin retaining the SC, it was strongly expressed in the SS of the transplanted skin lacking the SC and the non-keratinized oral mucosa, and in the SS and SG of the keratinized oral mucosa. Loricrin, which was expressed in the SG of normal skin, the transplanted skin retaining the SC and the keratinized oral mucosa, was not detected in the transplanted skin lacking the SC or in the non-keratinized oral mucosa. Immunoelectron microscopy confirmed the ultrastructural localization of SPR3 directly under the cytoplasmic membrane of keratinocytes of the transplanted skin lacking the SC and that of the oral mucosa. Conclusions The altered expression of SPR2, SPR3 and loricrin reflects the possible adaptation of epidermal keratinocytes in the new environment of the oral cavity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2133.2003.05288.x
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  • 5
    Electronic Resource
    Electronic Resource
    Vascular smooth muscle cells and pericytes express MMP-1, MMP-9, TIMP-1 and type I procollagen in inflammatory bowel disease (2001)
    Oxford UK : Blackwell Science Ltd
    Histopathology 39 (2001), S. 0 
    Details
    ISSN: 1365-2559
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Vascular smooth muscle cells and pericytes express MMP-1, MMP-9, TIMP-1 and type I procollagen in inflammatory bowel disease Aims: Matrix metalloproteinases (MMPs) are involved in tissue remodelling, which is one of the important aspects of inflammatory disease. To assess the balance between the matrix degradation and production, we analysed the in situ expression of MMP-1, -3, -8 and -9, tissue inhibitor of metalloproteinases (TIMP)-1 and -2, and type I procollagen (PC-I) in inflammatory bowel disease. Methods and results: Immunohistochemistry using frozen sections was performed in 17 patients with ulcerative colitis (UC) and 16 with Crohn’s disease (CD). In both UC and CD, MMPs and TIMPs were expressed by inflammatory cells as well as by fibroblastic cells most prominently in actively inflamed areas in ulcer bases, but sparsely in intact inflamed mucosa in both UC and CD. In UC, inflamed mucosa with erosions expressed these substances focally. Fibroblasts also expressed PC-I. We identified that vascular smooth muscle cells of venules in ulcer bases expressed MMP-1 and -9, TIMP-1 and PC-I. These venules also expressed E-selectin, a cell adhesion molecule to facilitate the leucocyte extravasation, and vascular endothelial growth factor (VEGF) receptor 2, consistent with their property of newly formed vessels. Conclusions: Our results suggest that MMPs are involved in the tissue remodelling, angiogenesis and promotion of leucocyte extravasation in the actively inflamed area in the ulcer base in both UC and CD. MMP-1 expression in the mucosa may be related to the initial step of ulceration in UC. Therapeutic manipulation of extracellular matrix turnover would be an effective therapy to alleviate active inflammation and accelerate ulcer healing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2559.2001.01142.x
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  • 6
    Electronic Resource
    Electronic Resource
    The binding of human milk lactoferrin to immunoglobulin A
    Amsterdam : Elsevier
    FEBS Letters 168 (1984), S. 203-207 
    Details
    ISSN: 0014-5793
    Keywords: IgA affinity column ; Lactoferrin ; Secretory IgA ; Secretory component
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0014-5793(84)80246-X
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  • 7
    Electronic Resource
    Electronic Resource
    Secretory Component in Immunoglobulin Deficiency: an Immunoelectron Microscopic Study of Intestinal Epithelium (1980)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 12 (1980), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The ultrastructural sites of secretory component (SC) in the intestinal epithelium of severely immunoglobulin-deficient and immunologically intact persons were compared. SC was found in the same sites in the immunoglobulin-deficient and normal epithelium: perinuclear spaces, rough endoplasmic reticulum, Golgi completes, external surfaces and endocytic invaginations of the basolateral plasmalemma, and in membranous vesicles throughout the cytoplasm. These observations suggest that the synthesis, external expression and transcellular migration of SC occur independent of the presence of immunoglobulins. The migration of SC from the basolateral cell surfaces to apical surfaces may be an inherent property of epithelial cells and the primary event in the translocation of polymeric immunoglobulins across the cells to external body fluids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1980.tb00078.x
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  • 8
    Electronic Resource
    Electronic Resource
    Immunoelectron microscopical localization of immunoglobulins, secretory component and J chain in the human minor salivary glands (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral pathology & medicine 17 (1988), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Localization of IgA, secretory component (SC) and J chain was investigated immunocytochemically in minor salivary glands of the lip and palate to define the mechanism involved in the transport of immunoglobulin A (slgA) into the saliva from the minor salivary glands. SC synthesis was detected in mucous acinar cells and ductal epithelial cells. Free SC is secreted into the saliva through secretory granules in the mucous acinar cells. Dimeric IgA containing J chain is translocated through these cells as slgA by a SC-medialed transport mechanism involving cytoplasmic vesicles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.1988.tb01303.x
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  • 9
    Electronic Resource
    Electronic Resource
    Interleukin-1β increases RANTES gene expression and production in synovial fibroblasts from human temporomandibular joint (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral pathology & medicine 33 (2004), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background:  Synovial fibroblasts of temporomandibular joint (TMJ) are poorly characterized, although they have important roles in progression of temporomandibular disorders (TMD). In this study, we investigated responses of synovial fibroblasts to interleukin (IL)-1β.Methods:  We examined gene expression profiles of synovial fibroblasts in response to IL-1β, using Affymetrix GeneChip. Regulated upon activation normal T-cell expressed and secreted (RANTES) gene expression was confirmed by polymerase chain reaction (PCR) and real-time PCR. RANTES protein levels were measured by enzyme-linked immunosorbent assay (ELISA).Results:  The RANTES was preferentially up-regulated in synovial fibroblasts by IL-1β. The increase in RANTES gene expression in response to IL-1β was confirmed by PCR and real-time PCR. Protein level of RANTES in synovial fibroblasts was also increased by IL-1β.Conclusions:  The RANTES, a cc-type chemokine, has chemotactic effects on lymphocytes and monocytes. Increased gene expression and protein production of RANTES in synovial fibroblasts, in response to IL-1β, may play an important role in recruitment of inflammatory cells into synovium and progression of synovitis in TMD.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.2004.00260.x
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  • 10
    Electronic Resource
    Electronic Resource
    Immunohistochemical analysis of cells in mucosal lesions of oral lichen planus (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral pathology & medicine 17 (1988), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: We immunohistochemically analyzed the immunological background of the pathogenesis of oral lichen planus (OLP). Abundant lymphocytes infiltrated OLP mucosal lesions, particularly in satellite cell necrosis (SCN) regions, and cytotoxic/suppressor T lymphocytes were predominant in seven of 10 OLP cases. Some carried IL-2 receptors, particularly in SCN. Keratinocytes of the mucosal lesions expressed both HLA-ABC and HLA-DR antigens. This suggests that activated cytotoxic/suppressor T lymphocytes may play a major role in cytotoxity to keratinocytes as effector cells in OLP. OKM1-, OKM5+, HLA-DR+ and IL-1+ cells, vascular endothelial cells and clusters of spindle-shaped or oval cells, which had the same surface characteristics as a monocyte macrophage subset, were abundant and, together with migrating lymphocytes, might play a role in the immune response in mucosal lesions of OLP.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.1988.tb01299.x
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