ISSN:
1617-4623
Keywords:
Recombinant DNA
;
Antibiotic production
;
Genetic organization
;
Directed mutant screen
;
Prodigiosin
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Fifteen mutants ofStreptomyces coelicolor A3(2) blocked in both the bipyrrole branch (redA) and a second site specific to the undecylprodigiosin pathway were characterized. Some of the mutants were ordered biosynthetically based on cosynthesis experiments. Complementation of each of the mutants with wild-type DNA cloned in low- and high-copy number plasmid vectors allowed the mutants to be separated into 12 new classes which are physically clustered within approximately 37 kb on theS. coelicolor genome. Early-step biosynthetic genes are centrally located and are flanked by later-step and regulatory genes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00260702
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