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  • 1
    Electronic Resource
    Electronic Resource
    Enzyme activities associated with arabitol and mannitol biosynthesis and catabolism in Schizophyllum commune (1976)
    Springer
    Archives of microbiology 107 (1976), S. 81-86 
    Details
    ISSN: 1432-072X
    Keywords: Schizophyllum commune ; Basidiospore germination ; Polyol dehydrogenases ; Polyacrylamide dise gel electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Enzymes of polyol metabolism were studied in basidiospore germination of Schizophyllum commune during periods of in vivo arabitol and mannitol pool depletion (growth on glucose-asparagine) and during their subsequent synthesis (growth on acetate-NH 4 + ). Optimal conditions for assays were established and specific activities of enzymes employing d-arabitol, d-mannitol, d-ribulose, d-fructose and d-xylulose as substrates were traced. Inquiries into the products formed during these reactions showed that d-ribulose generated arabitol while d-fructose produced mannitol with d-xylulose giving rise to xylitol. The dehydrogenase reactions were further investigated using polyacrylamide disc gel electrophoresis. Here was revealed the existence of at least two separate enzymatic activities pertaining to the catabolism of arabitol and mannitol. Also noted were the electrophoretic patterns when d-sorbitol, ribitol, xylitol and ethanol were used as substrates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00427871
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Differentiation in Coprinus lagopus (1975)
    Springer
    Archives of microbiology 105 (1975), S. 257-260 
    Details
    ISSN: 1432-072X
    Keywords: Coprinus lagopus (sensu Buller) ; Fruit-body expansion ; Stipe elongation ; Growth localization ; Autonomy of stipe development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fruit-body expansion was studied in Coprinus lagopus (sensu Buller) following surgical procedures. Elongation occurred after denuding mushroom caps of essentially all peripheral scales. Young primordia (1–5 mm) failed to develop after vertical bisection. Older primordia (e. g. 10 mm) expanded 3–4-fold after vertical bisection or quadrisection, underwent autolysis and basidiospore production. An amorphous brown gel in the stipe lumen disappeared during development of bisected primordia. Stipes isolated from primordia expanded autonomously and exhibited negative geotropism when incubated upside down or when the stipe apex was removed. Displacement of charcoal particles dusted on intact stipes revealed the most active zone of expansion to be the upper mid-region of the stipe. Segmented stipes likewise showed most active elongation in the mid-region. Vertically bisected stipes also expanded.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00447144
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Nutritional and temporal control of arabitol and mannitol accumulation in Geotrichum (1970)
    Springer
    Archives of microbiology 76 (1970), S. 65-73 
    Details
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Intracellular arabitol and mannitol accumulation is under nutritional and temporal control during arthrospore germination, vegetative growth, and arthrospore formation in Geotrichum. Arabitol is not produced if the glucose concentration in the medium is low. Arabitol is produced in large quantities in the cells if the carbon source is acetate or if the glucose level is above 10%. Low levels of glucose do not repress acetate induction of arabitol formation. Arabitol began to accumulate during spore swelling and vegetative growth in the presence of acetate. Mannitol appeared to serve as a carbon and energy reserve during starvation and arthrospore germination; the concentration of mannitol in vegetative cells remained barely detectable until sporulation commenced.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00409315
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  • 4
    Electronic Resource
    Electronic Resource
    Origin and ultrastructure of intra-hyphal hyphae in Trichophyton terrestre and T. rubrum (1975)
    Springer
    Archives of microbiology 106 (1975), S. 195-200 
    Details
    ISSN: 1432-072X
    Keywords: Trichophyton terrestre ; Trichophyton rubrum ; Hyphal fusions ; Origin of intra-hyphal hyphae ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cell observation chamber was designed to perform continuous photomicroscopic observations of hyphal anastomosis and the origin of intra-hyphal hyphae in Trichophyton terrestre and T. rubrum. These data were correlated with ultrastructural features of intra-hyphal hyphae. Hyphal fusions occurred commonly in either species of Trichophyton when incubated alone. In T. terrestre, empty hyphal segments adjoined by live units were invaded at the septa from both directions by new hyphal ingrowth. Continuous observations revealed that the intra-hyphal hyphae subsequently anastomosed via a lateral fusion peg. Similar intra-hyphal hyphae were shown in T. rubrum. Electron microscopic studies revealed ascomycetous septa in both conventional hyphae and intra-hyphal hyphae. For the latter, the cytoplasm and wall of the inner hypha were bounded by cytoplasmic organelles and another cell wall of the outer hypha.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00446523
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  • 5
    Electronic Resource
    Electronic Resource
    Control of erythritol dehydrogenase in Schizophyllum commune (1967)
    Springer
    Archives of microbiology 56 (1967), S. 22-30 
    Details
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An NAD-dependent erythritol dehydrogenase was detected in cell-extracts of basidiospore germinants of Schizophyllum commune following culture on either meso-erythritol or glycerol as sole carbon sources. Induction of erythritol dehydrogenase was also observed in purely vegetative mycelium (str. 845 or str. 699). Erythritol dehydrogenase was not observed in ungerminated basidiospores or germinants which arose on d-glucose, d-mannitol, sorbitol, ribitol, xylitol, d-arabitol or l-arabitol. NAD-coupled polyol dehydrogenases for all the latter sugar alcohols were observed in ungerminated basidiospores, germinants, and vegetative mycelium of S. commune cultured on d-glucose. Basidiospore germination on d-glucose plus meso-erythritol led to a 90% decrease in erythritol dehydrogenase and the specific activity of ribitol dehydrogenase was directly comparable to that seen in d-glucose germinants. Storage experiments of crude extracts of meso-erythritol germinants indicated differential enzyme decay of dehydrogenases for d-mannitol, sorbitol and erythritol while the respective enzymes could be further distinguished by heat-stability as well as preferential utilization of analogues of NAD. DEAE-cellulose column chromatography led to separation of sorbitol dehydrogenase which was also active with xylitol, erythritol dehydrogenase, and mannitol dehydrogenase which was also active with d-arabitol.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00406051
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  • 6
    Electronic Resource
    Electronic Resource
    Cytochrome System of Streptomycetes (1959)
    [s.l.] : Nature Publishing Group
    Nature 184 (1959), S. 1954-1955 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Mycelium was grown in a liqiud medium ('Bacto' nutrient broth, glycerol, pS. 7) on a rotary shaker at 30 C. The endogenous respiration of S. coelicolor 9 S. fradiae, S. lavendulae, S. griseus and S. rimosus pellets was inhibited between 40 and 70 per cent by IQ-*M hydrogen cyanide. In order to ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/1841954a0
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