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  • 1
    Electronic Resource
    Electronic Resource
    Cloning and characterization of a Schizophyllum gene with Aβ6 mating-type activity (1996)
    Springer
    Current genetics 29 (1996), S. 136-142 
    Details
    ISSN: 1432-0983
    Keywords: Key words Schizophyllum commune ; Mating type ; Homeodomain
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The A-pathway of development in the basidiomycete fungus Schizophyllum commune may be activated by either of two mating-type loci, Aα and Aβ. Aα consists of two multiallelic genes, Y and Z. Y contains a putative homeodomain; Z contains a homeodomain-related region. Non-self combinations of Y and Z form heteromultimers which are thought to be transcription factors of developmental genes. To more completely understand A-regulated development it is necessary to address the issue of functional redundancy, i.e., how do two different mating loci, Aα and Aβ, both manage to regulate the same pathway. Here we report the structure of a gene with Aβ6 activity. This gene, denoted AβV6, encodes a deduced polypeptide of 640 amino-acids with a homeodomain motif. V6 also contains a 20-amino acid sequence that is conserved in Aα Y1, Y3 and Y4. Except for the homeodomain and the conserved sequence, the deduced V6 polypeptide shows no significant identity to AαY, AαZ, or other known proteins. The presence of a homeodomain suggests that V, like Y and Z, may be a regulatory protein for genes in the A-pathway. Thus while Aα and Aβ encode different proteins, the general mechanism by which Aα and Aβ components signal A-regulated development may be similar.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050028
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  • 2
    Electronic Resource
    Electronic Resource
    The Aα mating-type locus of Schizophyllum commune: structure and function of gene X (1996)
    Springer
    Current genetics 29 (1996), S. 143-149 
    Details
    ISSN: 1432-0983
    Keywords: Key words  Schizophyllum commune ; Mating type ; Gene replacement ; Gene disruption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   Gene X maps immediately right of gene Y at the Aα mating-type locus in Schizophyllum commune. Alleles X1, X3 and X4 were isolated, subcloned, and sequenced. The structure of the alleles and their relationship to the Aα locus is described. The deduced X isoforms possess no recognized motifs common to other polypeptides and no significant similarity to any sequences in the protein databases. X alleles do not activate A-regulated development when transformed into recipient strains possessing different Aα mating types or when these transformants are mated with tester strains. An X1-disrupted construction yielded a high frequency (33%) of homologous gene replacements. X-disrupted mutants have wild-type phenotypes and mate normally. Both the functional analyses and sequence data for X1, X3, and X4 suggest that the right boundary of the Aα mating-type locus falls between AαY and X. We propose that the Z and Y genes constitute the Aα locus in its entirety.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050029
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  • 3
    Electronic Resource
    Electronic Resource
    The Aα mating-type locus ofSchizophyflum commune: Structure and function of geneX (1996)
    Springer
    Current genetics 29 (1996), S. 143-149 
    Details
    ISSN: 1432-0983
    Keywords: Schizophyllum commune ; Mating type ; Gene replacement ; Gene disruption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract GeneX maps immediately right of geneY at the Aα mating-type locus inSchizophyllum commune. AllelesX1, X3 andX4 were isolated, subcloned, and sequenced. The structure of the alleles and their relationship to the Aα locus is described. The deducedX isoforms possess no recognized motifs common to other polypeptides and no significant similarity to any sequences in the protein databases.X alleles do not activate A-regulated development when transformed into recipient strains possessing different Aα mating types or when these transformants are mated with tester strains. AnX1-disrupted construction yielded a high frequency (33%) of homologous gene replacements.X-disrupted mutants have wild-type phenotypes and mate normally. Both the functional analyses and sequence data forX1,X3, andX4 suggest that the right boundary of the Aα mating-type locus falls betweenAαY andX. We propose that theZ andY genes constitute the Aoc locus in its entirety.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02221578
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  • 4
    Electronic Resource
    Electronic Resource
    Cloning and characterization of aSchizophyllum gene withAβ6 mating-type activity (1996)
    Springer
    Current genetics 29 (1996), S. 136-142 
    Details
    ISSN: 1432-0983
    Keywords: Schizophyllum commune ; Mating type ; Homeodomain
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The A-pathway of development in the basidiomycete fungusSchizophyllum commune may be activated by either of two mating-type loci,Aξ andAβ Aα consists of two multiallelic genes,Y andZ. Y contains a putative homeodomain; Z contains a homeodomain-related region. Non- self combinations of Y and Z form heteromultimers which are thought to be transcription factors of developmental genes. To more completely understand A-regulated development it is necessary to address the issue of functional redundancy, i.e., how do two different mating loci,Aα andAβ, both manage to regulate the same pathway. Here we report the structure of a gene withAβ6 activity. This gene, denotedAβV6, encodes a deduced polypeptide of 640 amino-acids with a homeodomain motif. V6 also contains a 20-amino acid sequence that is conserved in Aα Y1, Y3 and Y4. Except for the homeodomain and the conserved sequence, the deduced V6 polypeptide shows no significant identity to AαY, AαZ, or other known proteins. The presence of a homeodomain suggests that V, like Y and Z, may be a regulatory protein for genes in the A-pathway. Thus whileAα andAβ encode different proteins, the general mechanism by which Aα and Aβ components signal A-regulated development may be similar.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02221577
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  • 5
    Electronic Resource
    Electronic Resource
    Developmental regulation of the methylation of the ribosomal DNA in the basidiomycete fungusSchizophyllum commune (1988)
    Springer
    Current genetics 14 (1988), S. 105-111 
    Details
    ISSN: 1432-0983
    Keywords: Schizophyllum ; Methylation ; Ribosomal DNA ; Developmental regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The ribosomal DNA (rDNA) ofSchizophyllum commune was shown previously to be highly methylated (Specht et al. 1984). To investigate the role of methylation of rDNA, a map of the recognition sites (CCGG) of the methylation-sensitive restriction endonucleases, MspI and Hpall, was prepared for the rDNA ofS. commune strain 4–40. The incidence of methylation at these CCGG sequences was analyzed. Our analyses demonstrate that either of the cytosines of the CCGG sequence can be methylated in Schizophyllum, however methylation of the internal cytosine is more frequent than methylation of the external cytosine. The external cytosines of CCGG sequences near the rDNA transcriptional initiation site are less frequently methylated than those else-where in the rDNA. The methylation of these sequences has been shown to be developmentally regulated inS. commune. We find that these sequences are more highly methylated in dikaryonic than homokaryotic cells. We propose that methylation of the rDNA may have a role in regulating the transcription of rRNA inS. commune.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00569333
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  • 6
    Electronic Resource
    Electronic Resource
    Isolation of the DNA sequence coding indole-3-glycerol phosphate synthetase and Phosphoribosylanthranilate isomerase of Schizophyllum commune (1986)
    Springer
    Current genetics 10 (1986), S. 909-913 
    Details
    ISSN: 1432-0983
    Keywords: E. coli trpC ; Schizophyllum TRP1 ; Complementation ; Gene isolation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A Schizophyllum gene library was made in plasmid pRK9, Plasmids from this library were tested for their ability to complement several auxotrophic mutations of Escherichia coli. The goal was to isolate a Schizophyllum auxotrophic gene that could be used to transform a corresponding Schizophyllum auxotrophic mutant to prototrophy. Complementation was observed only for E. coli trpC indole 3-glycerol phosphate synthetase (IGPS) and phosphoribosyl-anthranilate isomerase (PRAI) mutations. Plasmids with a Schizophyllum sequence coding for both IGPS and PRAI activities were recovered from E. coli transformants. Expression of the Schizophyllum gene (TRP1) in E. coli is probably dependent on the Serratia marcescens promoter of plasmid pRK9. The DNA sequence containing the Schizophyllum TRP1 gene was not obviously rearranged in cloning.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00398288
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  • 7
    Electronic Resource
    Electronic Resource
    Erratum (1988)
    Springer
    Current genetics 13 (1988), S. 271-271 
    Details
    ISSN: 1432-0983
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387775
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  • 8
    Electronic Resource
    Electronic Resource
    The isolation of specific genes from the basidiomycete Schizophyllum commune (1987)
    Springer
    Current genetics 12 (1987), S. 547-554 
    Details
    ISSN: 1432-0983
    Keywords: Schizophyllum commune ; Transformation ; Gene isolation ; Basidiomycetes ; Recombinant DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have developed a routine way to isolate genes directly from the basidiomycete fungus, Schizophyllum commune. Plasmid DNA from a genomic gene library was used to isolate five specific genes by complementation of Schizophyllum mutations via transformation. The mutant strains were deficient in the ability to synthesize either adenine (ade2 and ade5), uracil (ural, encoding orotidine-5′-phosphate decarboxylase; OMPdecase), tryptophan (rpl, encoding indole-3-glycerol phosphate synthetase; IGPS) or para aminobenzoic acid (pab1). In each case, Southern analysis revealed that transformation to prototrophy was concomitant with the integration of vector sequence into the genome of the S. commune mutant. Total DNA from transformants was restricted, religated, and used to transform E. coli. Ampicillin resistant plasmids were recovered from E. coli and tested for their ability to transform the corresponding mutant of S. commune. Plasmids complementing the ade2, adeS, pabl, trpl, and ural mutations were recovered.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00419565
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  • 9
    Electronic Resource
    Electronic Resource
    Nucleotide base sequence of the mitochondrial COIII gene of Schizophyllum commune (1988)
    Springer
    Current genetics 14 (1988), S. 401-403 
    Details
    ISSN: 1432-0983
    Keywords: Schizophyllum commune ; COIII ; mtDNA ; Basidiomycete
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The cytochrome oxidase subunit III gene (COIII) of the Badidiomycete S. commune has been identified, cloned, and sequenced. The gene contains no introns, is AT-rich (69%) and exhibits a high degree of similarity to sequences from Ascomycetes. While most mitochondrial genes use both TGA and TGG to specify tryptophan, the COIII gene of Schizophyllum uses TGG exclusively. Translation requires no deviation from the universal code.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00419999
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  • 10
    Electronic Resource
    Electronic Resource
    Organization of the ribosomal RNA genes of Schizophyllum commune (1988)
    Springer
    Current genetics 13 (1988), S. 417-424 
    Details
    ISSN: 1432-0983
    Keywords: Schizophyllum ; Ribosomal DNA ; Gene organization ; Length polymorphisms ; Transcriptional initiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The 18, 5.8, 25 and 5S ribosomal RNA (rRNA) cistrons have been mapped on the ribosomal DNA (rDNA) unit repeat of Schizophyllum commune strain 4–40. These genes are spatially ordered in the sequence given. The presence of a large primary precursor rRNA which is processed to form the mature 18, 5.8 and 25S rRNAs has been demonstrated. We have mapped the site of transcriptional initiation for this rRNA primary precursor. The sequence surrounding this site has been determined and shown to be highly conserved, with considerable identity to those in Neurospora crassa and Dictyostelium discoideum. The direction of transcription of the rRNA genes has been determined. The 5S rRNA cistron is transcribed in the same direction as the other rRNAs, however it is not transcribed as a part of the large primary precursor. The previously identified rDNA strain-specific length polymorphisms (Specht et al. 1984) are shown to be located within the transcribed region of the rDNA unit repeat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00365663
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