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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Theriogenology 22 (1984), S. 489-495 
    ISSN: 0093-691X
    Keywords: LH ; Sheep ; circadian rhythm ; oestrous behaviour
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science, Ltd
    European journal of neuroscience 19 (2004), S. 0 
    ISSN: 1460-9568
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Our aim was to study the role of the olfactory amygdala (medial and cortical nuclei) and the ventromedial nucleus of the hypothalamus (VMN) in the ability of the male odour or live males to induce a release of luteinizing hormone in anoestrus ewes. To achieve this, we temporarily blocked the activity of these structures by localized retrodialysis administration of the anaesthetic lidocaine. The effect of ram odour on the secretion of luteinizing hormone was completely blocked by inactivation of the cortical nucleus of the amygdala. In contrast, inactivation of part of the accessory olfactory system (the medial nucleus of the amygdala or the VMN) had no effect. In the presence of the male, lidocaine never impaired the endocrine response of the ewes. These results show that modulation of reproduction by the sexual partner even through pheromonal cues does not occur via the direct circuit of the accessory system. On the contrary, the cortical nucleus of the amygdala is absolutely necessary for the treatment of and/or the response to the male olfactory signal but this structure can be bypassed when other sensory cues are available.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1365-2826
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Oestrogen plays a key role in the regulation of the endocrine and behavioural events associated with the oestrous cycle. It is important, therefore, to know the location of neurones receptive to this steroid and to know whether their distribution varies with the oestrous cycle. We have undertaken experiments to identify the location of oestrogen receptors (ER) within the preoptico-hypothalamic continuum of ovariectomized ewes submitted to a variety of different hormone replacement regimes which mimic the different stages of the oestrous cycle. We used a monoclonal antibody to ER and detected receptors with immunohistological methods in the non-vascular part of the organum vasculosum of the lamina terminalis, the lateral septum, the medial preoptic area, the supraoptic, suprachiasmatic and arcuate (ARC) nuclei, the ventromedial hypothalamus (HVM) and in the region close to the mamillari recess. ER neurones were scarce or absent from the anterior hypothalamus and the paraventricular nucleus. The density of ER staining in the HVM, but in no other localization, was found to be higher, and in a more lateral position, during the induced luteal phase (progesterone treatment) than during the follicular phase (7 days of progesterone treatment followed by oestradiol) or in the ovariectomized female. In all areas studied, except for the ARC, the apparent surface area of the nucleus in ER immunoreactive cells varied with hormonal treatment. These data, and especially those in the HVM, contribute towards our understanding of how steroids may act in the ovine to control sexual behaviour.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    European journal of soil science 45 (1994), S. 0 
    ISSN: 1365-2389
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Changes in soil and biogeochemical processes following the replacement, 60 years ago, of part of a deciduous forest by a coniferous stand were evaluated by (i) a balance-sheet approach using soil and biomass element content analyses and the mineral flux, and (ii) the inclusion of test-mineral and resin bags in an integrative experimental approach.The comparison of soils under different stands shows that the change to spruce is inducing physical, chemical and biological modifications in soil properties. Changes in the humus layers lead to a reduction in mineralization and the mobilization of active organic acids in the system. Under spruce, the soil structural stability is reduced as fine clay is dispersed. Soil acidification is increased, linked to a decrease in soil pH and desaturation of the soil exchange complex. Soil mineralogy also indicates this tendency, particularly with an increase in the stability of Al intergrades. The biogeochemical cycle is modified especially for N, S and Al. with large changes in the internal fluxes between the ecosystem components, but the modification of the input-output balance due to spruce introduction is large only for S.The acidification is partly due to a change in litter quality, which inhibits biological activity, and partly because, during the dormant season, evergreen species intercept ‘dry and occult’ depositions from the atmosphere containing acidic or potentially acidic components.Use of test-mineral bags improves understanding and interpretation of the current soil mechanisms. The test mineral, a vermiculite, introduced into the soil or placed in the lysimeter flux very quickly undergoes measurable transformation characterized by desaturation and fixation of non-exchangeable Al in the interlayer zone, easily identified by Al speciation. The acidifying effect of spruce is clearly demonstrated by the experimental approach used, including the characterization of soil solutions by resin bags.All the techniques are complementary and could be used together or individually, depending on the aim of the study.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science, Ltd
    Journal of neuroendocrinology 15 (2003), S. 0 
    ISSN: 1365-2826
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In sheep, essentially all the neurokinin B (NKB) neurones of the infundibular nucleus express oestradiol receptor α, and analysis of female and male brains has revealed an exceptionally marked female-dominant sex difference in the numbers of NKB neurones in the infundibular nucleus. This neuronal population is located in an oestradiol-sensitive brain area involved in the control of gonadotropin-releasing hormone (GnRH) secretion and oestrous behaviour, but its physiological role is poorly documented. The aim of the present study was to analyse NKB mRNA expression at a crucial time when the steroid has stimulated the pathways leading to the induction of these two events. After cloning a specific ovine NKB antisense riboprobe, we examined the effects of a short oestradiol treatment (4 h subcutaneously) on the expression of NKB mRNA in the caudal part of the infundibular nucleus of progesterone-primed ovariectomized ewes. We demonstrated that oestradiol decreased both the level of NKB mRNA expression (34%) and the number of cells containing NKB mRNA (43%). Oestradiol acts strongly on these NKB cells in the short term. We suggest that this early change in NKB mRNA expression during the preovulatory period might be involved in the control of the induction of GnRH secretion or oestrous behaviour.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neuroendocrinology 5 (1993), S. 0 
    ISSN: 1365-2826
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In vivo microdialysis and retrodialysis were used to investigate the role of oxytocin (OXY) release in the mediobasal hypothalamus (MBH) of the ewe in the control of sexual receptivity. Initial experiments showed that OXY release was significantly increased in ovariectomized animals treated with progesterone and oestradiol when they were sexually receptive towards males and received intromissions. No such increases were seen during tests where the ewes were receptive but the males were prevented from achieving intromission. By contrast, OXY release was significantly reduced in tests where the ewes were not receptive to the male. In a second experiment artificial vaginocervical stimulation (VCS) was found to significantly increase OXY release when the animals were treated with oestradiol and this effect was potentiated by progesterone priming. OXY release in the MBH was not significantly altered by VCS in the presence of progesterone priming alone. Plasma OXY concentrations were significantly increased by VCS following all three hormone treatments but no one treatment was significantly more effective than another. Noradrenaline release in the MBH was only significantly increased following VCS when progesterone priming was given before oestradiol treatment. No effects of VCS on release of GABA, glutamate or dopamine were seen but their basal concentrations were significantly increased by the combined steroid treatment compared to oestradiol alone. In a third experiment it was found that OXY (10 μM) infused bilaterally into the MBH of receptive ewes, by retrodialysis, significantly decreased sexual receptivity and increased the release of noradrenaline and GABA. Finally, in a fourth experiment is was shown that multiple intromissions significantly reduced sexual receptivity.These results show that OXY release in the MBH is increased by natural or artificial VCS and that this is modulated by sex steroids. The inhibitory action of OXY infusions into the MBH on sexual receptivity suggests that a functional role of this peptide within this brain region might be to mediate decreases in sexual receptivity which we observed following multiple intromissions. It is probable that the behavioural effects of this peptide are primarily mediated directly rather than through a modulation of noradrenaline and GABA release.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Applied Animal Behaviour Science 13 (1984), S. 173 
    ISSN: 0168-1591
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Applied Animal Behaviour Science 17 (1987), S. 289-304 
    ISSN: 0168-1591
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 0018-506X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 0018-506X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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