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  • 1
    Electronic Resource
    Electronic Resource
    In situ hybridization studies of stromelysin and tissue inhibitor of metalloproteinase 1 in IgA nephropathy (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Nephrology 1 (1995), S. 0 
    Details
    ISSN: 1440-1797
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary: Accumulation of the extracellular matrix (ECM) in IgA nephropathy (IgAN) is thought to cause deterioration of glomerular function. Stromelysin and tissue inhibitor of matrix proteinase 1 (TIMP1) may play an important role in the turnover of the glomerular ECM. However, the expression of these enzymes in human renal tissues remains undefined. In the present study, non-radioactive in situ mRNA hybridization, which permitted the analysis at a cellular level, was performed to localize stromelysin and TIMP1 in renal tissue of IgAN. We also determined the percentage of cells positive for stromelysin or TIMP1 mRNA among intraglomerular cells. A total of 16 patients with IgAN were examined, including eight patients with severe histopathological changes and eight with mild changes. Three patients without glomerular disease were also studied. Stromelysin and TIMP1 mRNA were weakly expressed in the mesangium of normal kidneys and IgAN renal tissues with mild damage. However, the expression of both mRNA was significantly increased in the area of mesangial proliferation, in glomerular epithelial cells and in Bowman's capsule of advanced lesions. Several cells in the area of mesangial proliferation were double positive for stromelysin and TIMP1 mRNA, while certain cells positive for stromelysin mRNA did not express TIMP1 mRNA. In the interstitium, epithelial cells of certain tubules and some mononuclear cells were positively stained for these mRNA, especially in advanced lesions. Our results indicated that stromelysin and TIMP1 genes were expressed in glomerular resident cells, tubular epithelial cells and infiltrated mononuclear cells in IgAN, and their expression was enhanced in advanced tissue damage. the demonstration of a co-expression and discordant expression of the genes indicates that each gene expression may be regulated in a cell type-specific manner and that it could also be altered by cellular environmental factors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1440-1797.1995.tb00017.x
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  • 2
    Electronic Resource
    Electronic Resource
    Systemic lupus erythematosus complicated with microangiopathic hemolytic anemia, acute renal failure, and central nervous system disorder, treated successfully with corticosteroids and plasmapheresis (1997)
    Springer
    Clinical and experimental nephrology 1 (1997), S. 229-233 
    Details
    ISSN: 1437-7799
    Keywords: systemic lupus erythematosus ; microangiopathic hemolytic anemia ; thrombotic thrombocytopenic purpura ; plasmapheresis ; acute renal failure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We report on a 15-year-old girl who had systemic lupus erythematosus (SLE), hemolytic anemia, thrombocytopenia, acute renal failure, and central nervous system disorder including an episode of convulsions. Red cell fragmentation was observed in the peripheral blood, but only a slight abnormality was noted in the coagulative/fibrinolytic system. She was treated with corticosteroid pulse therapy, hemodialysis, and plasmapheresis and anticoagulants. The severe anemia and thrombocytopenia responded to immunoadsorption. The renal biopsy specimen showed a thickening of the small arteries with a narrowing of the lumens, and an immunofluorescent study revealed deposits of fibrinogen in the renal blood vessels. A provisional diagnosis of SLE with thrombotic thrombocytopenic purpura was made. A magnetic resonance image of the brain obtained at the time of a convulsion showed multiple, scattered high-intensity areas on the rim of the cerebral cortex; these disappeared after treatment. These findings were strikingly similar to those observed in central nervous system lupus. We describe a case of SLE with microangiopathic hemolytic anemia that provides an insight into the mechanisms of thrombotic thrombocytopenic purpura and SLE that affect the central nervous system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02480700
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    Paper (German National Licenses)
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