ISSN:
1432-203X
Keywords:
Key Words: Garden egg –Solanum gilo– Transformation
;
Abbreviations: MS, Murashige and Skoog (1962) medium; 2,4-D, 2,4-Dichlorophenoxyacetic acid; NPTII, neomycin phosphotransferase; GUS, β-glucuronidase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract. An efficient system for Agrobacterium tumefaciens-mediated transformation of Solanum gilo was established. The marker genes for kanamycin resistance and β-glucuronidase expression were introduced. A comparison between cotyledon and hypocotyl explants showed that while regeneration was better from hypocotyl explants, cotyledon explants gave better transformation efficiency (46% vs. 32%). Four levels of kanamycin selection (100, 150, 200 and 250 mg/l) were tested for effect on transformation efficiency with each type of explant. Lower levels of kanamycin worked better using cotyledon explants, while higher levels of kanamycin worked better for hypocotyl explants. All nin T0 plants tested for expression of the kan r gene were positive. The progeny of three of these plants showed a pattern of classical Mendelian inheritance (3 to 1) for both the kan r and the β-glucuronidase genes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050078
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