ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
A comparison of total extractable protein, nonprotein nitrogen (NPN) and disc- and SDS-gel electrophoresis from aseptic control porcine muscle with similar samples after inoculation with C pevfrirzgens and incubation at 37°C for 0, 1, 2, 4 and 7 days demonstrated that this organism degraded both the sarcoplasmic and myofibrillar proteins. Incubation with C perkingens increased the amount of NPN, indicating proteolytic break-down into small nitrogen containing compounds, which was confirmed by a decrease in the sarcoplasmic protein fraction. Disc-gel electrophoresis of the sarcoplasmic extract from samples treated with C. perfringens revealed the presence of several new protein peaks, presumably degradation products, while some other protein peaks diminished in size. Preferential degradation of troponin by C. perfringens was confirmed by SDS-(sodium dodecylsulfate) gel electrophoresis, whereas, a reduction in the amount of actin was observed by disc-gel electrophoresis in urea but could not be confirmed with SDS-electrophoresis. Increases in the size of some other protein peaks were noted, which were attributed to comigration of degradation products with certain of the previously existing proteins.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.1976.tb14385.x
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