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  • 1
    Electronic Resource
    Electronic Resource
    Lysogeny in the blue-green algaNostoc muscorum (1978)
    Springer
    Archives of microbiology 117 (1978), S. 265-268 
    Details
    ISSN: 1432-072X
    Keywords: Nostoc muscorum ; Blue-green Algal virus ; N-1 ; Lysogeny ; Induction ; Heat ; Mitomycin C
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Lysogens were isolated after confluent lysis of the blue-green algaNostoc muscorum by N-1 virus and characterised. The spontaneous liberation of active virus particles in lysogens was not lost after treating them with viricidal concentration of EDTA and virus titre obtained in their cultures was 〉5×103 PFU/ml. The virus adsorbed on the lysogens with a slower rate than on parent alga, although it followed the pattern of a first order reaction. The heat treatment (45°C for 14h) of the lysogens failed to induce lysis, whereas mitomycin C (1–2 μg/ml) was effective in inducing lysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00738545
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of temperature on the adsorption and one-step growth of the Nostoc virus N-1 (1977)
    Springer
    Archives of microbiology 115 (1977), S. 163-167 
    Details
    ISSN: 1432-072X
    Keywords: Nostoc virus N-1 ; Temperature ; Adsorption ; One-step growth ; Viral development ; Nostoc muscorum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This study was an attempt to observe the effects of temperature on adsorption and one-step growth of the virus N-1 infecting the nitrogen-fixing cyanobacterium Nostoc muscorum. Adsorption rate was found to be maximum at 40° C whereas no adsorption occurred at 10° C. The Q 10 value was about 2.03 and the energy of activation, Ea was 16.3 kcal/ mole for the adsorption process. The development cycle of the virus was temperature sensitive. With increase in temperature, a gradual increase in inhibition of virus yield i.e. 8.33% at 30° C, 35.3% at 35° C and complete inhibition at 40° C was observed. Out of 7 h latent period, the early 4 h were temperature sensitive and heat treatment had a reversible inhibitory effect on virus development. The temperature treatment did not affect the rise period but burst-size was reduced.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00406370
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of host aging, ions, and pH on the adsorption of the cyanovirus N-1 to Nostoc muscorum (1978)
    Springer
    Archives of microbiology 116 (1978), S. 289-292 
    Details
    ISSN: 1432-072X
    Keywords: Blue-green algal virus N-1 ; Adsorption ; Host cell aging ; Killed cells ; Saline magnesium chloride solution ; Amino acids ; pH ; Nostoc muscorum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The adsorption rate of the cyanovirus N-1 infecting the nitrogen-fixing blue-green alga Nostoc muscorum decreased with aging of algal cultures and the virus failed to adsorb to the dead host cells. The adsorption rate declined in saline magnesium chloride solution compared to that in algal growth medium. The addition of amino acids like L-tryptophan and L-phenylalanine failed to enhance the adsorption rate of the virus. Optimal pH of adsorption was 7.6 to 8.1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00417853
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  • 4
    Electronic Resource
    Electronic Resource
    Mutation to resistance for virusN-1 in the blue-green algaNostoc muscorum (1976)
    Springer
    Archives of virology 52 (1976), S. 85-90 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The spontaneous and nitrosoguanidine inducedN-1 virus resistant mutants of the blue-green algaNostoc muscorum were isolated with the average mutation frequency of 3.55×10−5 and 6.08×10−4 respectively. Of the surviving colonies 8 per cent became virus sensitive, whereas out of the remaining 92 per cent 9.3 per cent showed lysogenic behaviour and 13.3 per cent showed poor or no nitrogen fixing ability. Mutation to virus-resistance was independent of the presence of the virus. Spontaneous host range mutants of virus were not found in repeated experiments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01317867
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  • 5
    Electronic Resource
    Electronic Resource
    Reversion of virus N-1 resistant mutant of blue-green algaNostoc muscorum (1978)
    Springer
    Cellular and molecular life sciences 34 (1978), S. 1565-1565 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The reversion of N-1 virus resistant strain of the algaNostoc muscorum was studied by inoculating parent virus in the resistant culture at various incubations. A fraction of virus resistant cells reverted to wild sensitive type with the reversion rate of 3.99×10−6/cell/generation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02034675
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  • 6
    Electronic Resource
    Electronic Resource
    Genetical studies on the heterocyst and nitrogen fixation of the blue-green alga Nostoc muscorum (1978)
    Springer
    Molecular genetics and genomics 162 (1978), S. 203-211 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The short-trichome-forming, non-heterocystous and non-nitrogen-fixing (het − nif−) mutant of the nitrogen-fixing blue-green alga Nostoc muscorum was isolated by N-methyl-N′-nitro-N-nitrosoguanidine (NTG)-mutagenesis after penicillin enrichment technique and characterized. The mutant did not grow and fix nitrogen in combined-nitrogen-free medium while in nitrate-containing medium it grew well (‘K’=0.112/day, ‘G’=64.27 h), although its growth was comparatively poor than the parent alga (K=0.128/day; G=56.14 h). The mutant was stable and both the het − and nif − characters reverted to wild type (het + nif+) with the reversion frequency of 2.62×10-7. The het − nif− mutant tolerated 0.5 μg/ml of streptomycin sulphate on the agar medium and its streptomycin resistant mutant capable of growing in presence of 10μg/ml of streptomycin was isolated spontaneously with a frequency of 1.45×10-8. These streptomycin resistant isolates (het − nif− strR) resisted 100 μg/ml of streptomycin sulphate on the agar medium and 200 μg/ml in liquid medium. Spontaneous virus-resistant mutant of het − nif− strR was isolated with a mutation frequency of 4.02×10-4. The data of genetic recombination experiments suggested that there is transfer of both het and nif genes to het − nif− strain with the frequency of 2×10-6 to 2×10-5 simultaneously. There was increase in recombination frequency with increasing the incubation period. The virus-resistance marker is also transferred to the sensitive recipient.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267877
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