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  • 1
    Electronic Resource
    Electronic Resource
    Coupling of ion transport in green cells ofAtriplex spongiosa leaves to energy sources in the light and in the dark (1970)
    Springer
    The journal of membrane biology 2 (1970), S. 17-30 
    Details
    ISSN: 1432-1424
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The coupling of ion transport to energy sources in the light and in the dark in green cells ofAtriplex spongiosa leaves was investigated using light of different qualities, an inhibitor of electron transport (dichlorophenyl dimethyl urea), and an uncoupler (p-CF3O-carbonyl cyanide phenylhydrazone). Two different mechanisms of ion uptake were, distinguished. (1) A light-dependent Cl− pump which is linked to light-dependent K+ uptake. The energy for this pump is probably derived from photosynthetic electron transport or from nicotinamide adenine dinucleotide phosphate, reduced form. This mechanism is dichlorophenyl dimethyl urea-sensitive and enhanced by uncouplers. (2) A mechanism independent of light, which operates at the same rate in the light and in the dark. This mechanism is sensitive to uncouplers. It is probably aK−Na exchange mechanism since K+ and Cl− uptake and a small net uptake of H+ are balanced by Na+ loss.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01869847
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Microautoradiographic investigations of sulfate uptake by glands and epidermal cells of water lily (Nymphaea) leaves with special reference to the effect of poly-l-lysine (1971)
    Springer
    The journal of membrane biology 4 (1971), S. 395-407 
    Details
    ISSN: 1432-1424
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The uptake of35S-labelled sulfate ions into hydropote cells (densely cytoplasmic gland cells) and into epidermal cells (highly vacuolated cells) ofNymphaea leaves is dependent on metabolic energy. Only a very small fraction of the accumulated35S is incorporated into organic macromolecules during the experimental period. Both cell types exhibit a hyperbolic isotherm for35S uptake from labelled K2SO4 solutions over an external concentration range of 0 to 0.5mm. Although the gland and epidermal cells behave qualitatively similarly, the glands generally absorb about twice as much35S per unit area of sections of the cells as do the epidermal cells. At 3 °C, poly-l-lysine concentrations of 10−8 m and up to 10−7 m enhance35S uptake by the epidermal and gland cells for the first 7.5 hr after application of the poly-l-lysine. Samples treated with 5×10−7 m poly-l-lysine are indistinguishable from the controls over the same period. After longer periods of treatment with poly-l-lysine (7.5 to 24 hr), the rates of35S uptake were reduced by all poly-l-lysine concentrations between the range 10−8 to 5×10−7 m. After 7.5 hr of35S uptake, the control samples contained the smallest amount of label, but after an uptake period of 24 hr the amount of label in the controls is considerably larger than in samples treated with poly-l-lysine. The results suggest that poly-l-lysine increases the membrane permeability and alters the metabolic uptake of sulfate in both hydropotes and epidermal cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02431980
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Rapid and economical multiple base site-directed mutagenesis of double stranded plasmid DNA (1997)
    Springer
    Biotechnology techniques 11 (1997), S. 367-370 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract This method for the mutagenesis of ds-DNA, utilizing the best features of previously published protocols, incorporates a fragmentation procedure on the plasmid, thermostable enzymes and two transformations in E. coli. Screening of positive clones can begin after about two days. Insertions, deletions and substitutions of up to 50 bp are routinely obtained with 90-95% of clones positive as proven by sequencing. The cost is about one half to one third of equiv-alent commercial procedures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018496101751
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    Paper (German National Licenses)
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