ISSN:
1438-2199
Keywords:
Amino acids
;
Modified amino acids
;
HPLC
;
Edman reagent
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Methylated lysines (N ɛ -mono-methylated, N ɛ -di-methylated and N ɛ -tri-methylated) have been identified after derivatization with orthophthaldialdehyde (OPA) by using pre-column and post-column derivatization techniques. Also the N ɛ -acetylated lysine and N ɛ -formylated lysine have been identified by OPA post-column derivatization techniques but only in free form due to their instability under acidic conditions which are used for protein hydrolysis. Additionally, all the modified amino acids mentioned above have been derivatized with DABITC/PITC, an Edman reagent, and identified as DABTH-derivatives on thin-layer polyamide sheets.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01373045
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