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  • 1
    Electronic Resource
    Electronic Resource
    Lectin histochemistry of the esophagus in several mammalian species (1999)
    Springer
    Anatomy and embryology 200 (1999), S. 541-549 
    Details
    ISSN: 1432-0568
    Keywords: Key words Lectin ; Esophagus ; Histochemistry ; Sucrose octasulfate ; Mammals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The mucosa of the esophagus consists of stratified squamous epithelium that has a considerable resistance to injury. Intercellular glycoconjugates appear to constitute a major permeability barrier in the superficial portion of the esophageal mucosa. In the present study, we used a panel of lectins to investigate the differences in glycoconjugate production among different mammalian species. A battery of 12 lectins was used to study binding in sections from the esophagus of 6 mammalian species, including man. In general, the strongest staining was obtained in the stratum superficiale and the weakest staining in the stratum germinativum. In rabbit esophagus, exposure to pepsin/HCl produced a superficial damage to the epithelium, a considerable decrease in electrical resistance and a decreased staining of the esophageal epithelium with selected lectins. Pretreatment of the esophageal mucosa with sucrose octasulfate, a compound with protective properties, prevented, to some extent, the decrease in resistance and lectin staining.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004290050302
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Immunocytochemical distribution of the GABAB receptor splice variants GABAB R1a and R1b in the rat CNS and dorsal root ganglia (2000)
    Springer
    Anatomy and embryology 201 (2000), S. 1-13 
    Details
    ISSN: 1432-0568
    Keywords: Key words GABAB receptor ; CNS ; Dorsal root ganglia ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The anatomical distribution of the GABAB receptor (GBR) splice variants GBR1a and 1b in the CNS has not previously been studied. In the present study, distribution of the splice variants was mapped using immunohistochemistry. Polyclonal antibodies against splice variant unique epitopes were raised in rabbits. Affinity purified antibodies were used according to routine immunohistochemical procedures in sections from the rat CNS or dorsal root ganglia (DRG). The staining intensity was high in the cerebral cortex but lower in basal ganglia and the hippocampus. In the cerebellum, there was a marked difference in the distribution of GBR1a- and 1b-like immunoreactivity (LI). GBR1a-LI was preferentially localised in the granule cell layer whilst GBR1b-LI was mostly found in Purkinje cells and in the molecular layer. Cell bodies of the deep cerebellar nuclei stained for the GBR1a antibody while terminals surrounding the cell bodies were strongly labelled with the GBR1b antibody. A similar pre- vs postsynaptic pattern was seen in several nuclei ventral or caudal to the cerebellum (e.g. the cochlear nucleus, the facial nucleus, the spinal cord) but not in regions rostral to the cerebellum. In the spinal cord, strong labelling for both antibodies was seen in the dorsal horn. The GBR1b but not the GBR1a antibody stained tanycytes in the epithelium of the 3rd ventricle and in the central canal at the brain stem level. DRG neurons were positive for both the GBR1a and 1b antibody, but the former stained the cells much more intensely. Satellite cells were labelled with the GBR1b antibody. The most important aspect of these findings is that in some nuclei, GBR1b may mediate inhibition of transmitter release while in the same regions, GBR1a may mediate postsynaptic inhibition. Further, the observations support previous findings that GBR1b is the predominant splice variant in Purkinje cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00008224
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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