Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Sequence analysis of ospA genes shows homogeneity within Borrelia burgdorferi sensu stricto and Borrelia afzelii strains but reveals major subgroups within the Borrelia garinii species (1995)
    Springer
    Medical microbiology and immunology 184 (1995), S. 73-80 
    Details
    ISSN: 1432-1831
    Keywords: Borrelia burgdorferi, afzelii, garinii ; Outer surface protein A ; ospA genes ; ospA sequence analysis ; Outer surface protein A serotypes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The genes coding for the outer surface protein A (OspA) of 19 different Borrelia burgdorferi strains belonging to the seven OspA-serotypes 1–7, previously described [Wilske et al. (1993) J Clin Microbiol, 31: 340–350], have been investigated. B. burgdorferi sensu lato strains were chosen from various biological sources (ticks, human skin and cerebrospinal fluid) as well as different geographical origins (Germany, Slovenia, Austria, United States). The open reading frames of all ospA genes consist of 819–825 nucleotides corresponding to proteins of approximately 30 kDa. The ospA sequences obtained in this study and previous published studies were compared with the results from OspA serotyping with monoclonal antibodies. The classification into the seven OspA serotypes could be confirmed on a genetic basis (ospA genotypes 1–7) for all strains analyzed so far (n=29). In addition, one strain without OspA expression could be assigned to ospA genotype 2. Genetic stability could be proven for the ospA gene of B. burgdorferi strain PWudI after inocculation and reisolation from a gerbil. However, we found evidence for intragenic recombination by cluster analysis of ospA sequence data. Accordance of ospA genotype 1 strains with B. burgdorferi sensu stricto and ospA genotype 2 strains with B. afzelii, as well as the ospA genotype strains 3–7 with B. garinii was confirmed by pulsed-field gel electrophoresis of MluI-digested genomic DNA. B. garinii is not only more heterogenous in respect to the OspA-encoding genes, but shows moreover major subgroups formed by genotypes 4, 5 and 6 and genotypes 3 and 7, respectively. The latter group has not been described previously and is specifically recognized by an OspA-specific monoclonal antibody L32 1F7.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221390
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Disputed Interpretation of a Pronoun (1970)
    [s.l.] : Nature Publishing Group
    Nature 227 (1970), S. 641-641 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] SIR,-I make the following comment on Hammertori's letter (Nature, 227, 202; 1970). Technical accuracy in grammar may be necessary, but is not sufficient, for the construction of good language. The latter ensures that concepts are transferred unambiguously and free of distortion by the channel of ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/227641a0
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Electrical transport and optical properties of zirconium nitride/aluminum nitride multilayers (1991)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 69 (1991), S. 846-849 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: We have synthesized zirconium nitride/aluminum nitride multilayered thin films with a reactive sputtering technique. The electrical transport and optical properties of these multilayers have been examined by resistivity, Hall effect, and near-normal incidence reflectance and transmittance measurements. We show the existence of a metal-to-insulator transition and strong increase of Hall coefficient at small wavelength of composition modulation. The optical response of the multilayers is systematically modified from the behavior of the individual constituents. The measured optical response is in qualitative agreement with a simple effective-medium theory.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.347320
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Electronic spectrum of crystalline lithium fluoride
    Amsterdam : Elsevier
    Solid State Communications 5 (1967), S. v 
    Details
    ISSN: 0038-1098
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0038-1098(67)90807-1
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    facet.materialart.
    Unknown
    Über Friedrich Ehrenfeuchter (1963)
    Berlin : Periodicals Archive Online (PAO)
    Neue Zeitschrift für systematische Theologie und Religionsphilosophie. 5 (1963) 183 
    Details
    ISSN: 0028-3517
    Topics: Theology and Religious Studies
    URL: http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pao:&rft_dat=xri:pao:article:0146-1963-005-00-000010
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Immunological and molecular variability of OspA and OspC. implications forBorrelia vaccine development (1996)
    Springer
    Infection 24 (1996), S. 208-212 
    Details
    ISSN: 1439-0973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Borrelia burgdorferi sensu lato, der Erreger der Lyme-Borreliose, ist in Europa ausgesprochen heterogen. Da die äußeren Membranproteine OspA und OspC die Hauptkandidaten für eine Borrelien-Vakzine sind, wurde deren immunologische und molekulare Heterogenität untersucht. Aufgrund von Westernblotanalysen mit monoklonalen Antikörpern und Sequenzanalysen von PCR-amplifiziertem OspA und OspC fanden wir sieben verschiedene OspA- und 16 verschiedene OspC-Typen. Während Hautisolate (n=68) ganz vorwiegend (84%) OspA-Serotyp 2 (oderBorrelia afzelii) angehörten, waren Isolate aus Liquor cerebrospinalis und von Zecken (n=43 bzw. n=90) ausgesprochen heterogen im OspA-Typ mit Prävalenz der mitBorrelia garinii assoziierten OspA-Typen. OspA-Typ 4 fand sich oft bei Liquorisolaten (28%). Von Zecken wurde OspA-Typ 4 bisher nicht isoliert. Wie in einer anderen Studie berichtet, konnte jedoch Typ 4-OspA in Zecken mit der hochsensitiven PCR nachgewiesen werden.
    Notes: Summary Borrelia burgdorferi sensu lato, the etiological agent of Lyme borreliosis is considerably heterogeneous in Europe. Since the outer surface proteins OspA and OspC are the most promising candidates for aBorrelia vaccine the immunological heterogeneity of these proteins was investigated. By immunological analysis with monoclonal antibodies and sequence analysis of PCR amplified OspA and OspC at least seven and 16 different types, respectively, were found. Whereas skin isolates (n=68) were quite homogeneous (84% belonged to OspA-serotype 2 orBorrelia afzelii), isolates from human cerebrospinal fluid and from ticks (n=43 and n=90 respectively) were highly heterogeneous in their OspA-serotypes with prevalence of theBorrelia garinii associated types (about 70%). OspA-type 4 was often found among isolates from cerebrospinal fluid (28%). In ticks type 4 OspA has not been detected by culture so far. However, as reported in a previous study, type 4 OspA could be detected in ticks by the highly sensitive PCR technique.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01713341
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    2. Chirurgie im Spannungsfeld technischer Perfektion und Humanität (1982)
    Springer
    Langenbeck's archives of surgery 358 (1982), S. 47-52 
    Details
    ISSN: 1435-2451
    Keywords: Medical ethics ; Doctor-patient relationship ; Scientific-technical progress ; Medizinische Ethik ; Arzt-Patient-Verhältnis ; Wissenschaftlich-technischer Fortschritt
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammensetzung Die Symptome des gespannten Verhältnisses zwischen Technik und Humanität in der modernen Medizin — überhöhte Leistungserwartungen und Protest gegen angebliche Entmündigung durch Technik seitens der Patienten, Dissonanzen zwischen ärztlichen und wissenschaftlicher Aufgabe auf seiten der Ärzte —, fordern vom Chirurgen als exponiertem Repräsentanten medizinischen Fortschritts eine intensive Pflege des Vertrauensverhältnisses zum Patienten und die Bereitschaft zu fachlicher Kontrolle medizinischer Forschung, im Interesse einer produktiven Verarbeitung der Spannung.
    Notes: Summary The symptoms of the strained relationship between technology and humanity in modern medicine are excessive expectations with regard to achievement, protest on the part of patients against alleged dehumanization, and protest by doctors against dissonance between the tasks of the medical profession and science. — These symptoms require of the surgeon, as a representative of medical progress, that he intensively cultivate the trust-relationship with his patients and a readiness to accept the technical controls of medical research.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01271754
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Immunoblot using recombinant antigens derived from different genospecies of Borrelia burgdorferi sensu lato (1994)
    Springer
    Medical microbiology and immunology 183 (1994), S. 43-59 
    Details
    ISSN: 1432-1831
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Immunodominant proteins are variable in molecular and antigenic structure among different genospecies of Borrelia burgdorferi sensu lato. We have recently developed an immunoblot using five recombinant antigens: the chromosomal-encoded B. burgdorferi proteins p 100, the flagellin and an internal flagellin fragment thereof, and the plasmid-encoded outersurface proteins A (OspA) and C (OspC). In the present study the same antigens (derived from strain PKo, genospecies B. afzelii) were compared with the homologous recombinant proteins from strain B31 (genospecies B. burgdorferi sensu stricto) and with OspA, OspC and the internal flagellin fragment from strain PBi (genospecies B. garinii). Patients with neuroborreliosis (n=28) and patients with acrodermatitits chronica atrophicans (n=20) were investigated in the IgG immunoblot; the IgM immunoblot was performed only in patients with neuroborreliosis. There was a small increase in the detection rate of OspA-specific IgG or IgM antibodies using the different variants of recombinant OspA; however, OspA remained an insensitive antigen for antibody detection in Lyme borreliosis. The same was true to OspC-specific IgG antibodies. The sensitivity of OspC, which is the immunodominant antigen for IgM antibody detection, could not be increased using recombinant antigens derived from different strains. However, some sera which were negative in the recombinant immunoblot reacted with OspC in the conventional immunoblot using B. burgdorferi whole cell lysate as antigen. The most unexpected finding was the high degree of immunological heterogeneity of the internal flagellin fragments: IgG antibodies were detected in 18 of 48 patients using B31 fragments, in 25 of 48 using PKo fragments, in 23 of 48 using PBi fragments versus 33 of 48 when the three recombinant proteins were combined. PKo-derived fragments were more sensitive for antibody detection in patients with acrodermatitis chronica atrophicans, B31- and PBi-derived fragments for antibody detection in patients with neuroborreliosis. This is in agreement with the fact that isolates from patients with neuroborreliosis are predominantly belonging to the genospecies B. burgdorferi sensu stricto and B. garinii. For detection of IgM antibodies in sera from patients with neuroborreliosis, recombinant internal fragments derived from strains B31 and PBi were more sensitive than the PKo-derived fragment. The best discrimination between neuroborreliosis sera and control sera was achieved when the IgM blot was performed using recombinant internal flagellin fragments derived from strains PKo and PBi and OspC derived from B31 or PKo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00193630
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    The distribution of fluoride in some antarctic seals (1985)
    Springer
    Polar biology 5 (1985), S. 31-34 
    Details
    ISSN: 1432-2056
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The aim of this investigation was to find out why some antarctic seals tolerate fluoride levels in their food (krill) that would be toxic to man. The fluoride content of different soft and hard tissues, urine and feces from 16 L. carcinophagus, 13 L. weddelli and 2 A. tropicalis (urine only) were determined by the fluoride sensitive electrode or by a photometric method (urine). Results and conclusions are following: All soft tissues have low concentrations of fluoride in the same range as soft tissues from non antarctic and non krill eating marine or terrestrial vertebrates. But within the soft tissues there are significant differences in the fluoride content (blood: 0.001 ppm F/dw — brain: 9.1 ppm F/dw). A special tolerance for fluoride of soft tissues can be excluded. Compared with man or weddell-seals the crabeater-seals store two times more fluoride in their skeleton. Hard structures that stop their mineralisation early in the development like teeth have only a relatively low fluoride content. For the same reason only neglectable amounts of fluoride could be detected in hair. Fluoride excretion by the kidneys is efficient and is — besides the storage of fluoride in the skeleton — the second mechanism to guarantee a good blood homoiostasis for fluoride. Until now it is impossible to decide how much fluoride from the food is resorbed by the digestive tract.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00446042
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Molecular characterization of the p83/100 proteins of variousBorrelia burgdorferi sensu lato strains (1996)
    Springer
    Cellular and molecular life sciences 52 (1996), S. 305-306 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Conclusions As in OspA-serotyping experiments, theB garinii group (OspA-sterotype 3–7) showed highest diversity within this internal fragment of p83/100, whereas theB. afzelii group (OspA-type 2) and theB. burgdorferi sensu stricto group (OspA-type 1) were nearly identical. Determination of the size of the PCR products as well as restriction fragment length polymorphism analysis (DraI) can be used for classification into the three species ofB. burgdorferi sensu lato. Since p83/100 is chromosomally encoded, this protein might be a more stable marker for classification than the plasmid-encoded OspA. In contrast to the flagellin gene a subclassification of theB. garinii group is possible due to the diversity of the p83/100 internal fragment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01919522
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...