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  • 1
    Electronic Resource
    Electronic Resource
    The effect of monooleoylglycerol on insulin secretion from isolated perifused rat islets (1989)
    Springer
    Diabetologia 32 (1989), S. 360-364 
    Details
    ISSN: 1432-0428
    Keywords: Insulin secretion ; diacylglycerol ; isolated islets ; second messengers ; phosphoinositides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effect of monooleoylglycerol on cholecystokinin-and tolbutamide-induced insulin secretion was examined in isolated perifused rat islets. In the presence of 5.5 mmol/l glucose, addition of 10 nmol/l cholecystokinin or 50 μmol/l tolbutamide had practically no effect on insulin secretion. Combined tolbutamide and cholecystokinin led to a biphasic insulin secretory response which was significantly enhanced by addition of 50 μmol/l monooleoylglycerol, an inhibitor of diacylglycerol kinase. Monooleoylglycerol (50 μmol/l) alone had a minimal stimulatory effect on insulin release in the presence of 5.5 mmol/l glucose. Perifusion of islets with 1 μmol/l forskolin had no significant effect on basal insulin secretion in the presence of 5.5 mmol/l glucose, but markedly enhanced the responses to both cholecystokinin plus tolbutamide, and to the combination of cholecystokinin, tolbutamide and monooleoylglycerol. Lowering the glucose level to 2.75 mmol/l abolished the profound stimulatory effect to these agonist combinations on insulin release. Finally, monooleoylglycerol also enhanced the first and second phase insulin secretory responses induced by 20 mmol/l glucose. These results are discussed in relationship to the possible role of protein kinase C in mediating insulin secretion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00277259
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  • 2
    Electronic Resource
    Electronic Resource
    Fetal antigen 2 (FA2) in human fetal osteoblasts, cultured osteoblasts and osteogenic osteosarcoma cells (1992)
    Springer
    Anatomy and embryology 186 (1992), S. 271-274 
    Details
    ISSN: 1432-0568
    Keywords: Fetal antigen 2 ; Fetal bone formation ; Osteogenesis ; Osteoblasts ; Osteogenic osteosarcoma cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Immunohistochemical staining techniques used on an 11-week-old fetus showed that fetal antigen 2 (FA2) was present intracellularly in endochondral and perichondral osteoblasts, and the immunoreaction was extended into the adjacent bone matrix. Osteoclasts and chondroblasts were found to be FA2 negative. A granular perinuclear intracytoplasmic FA2 immunoreaction was found in cultured osteoblasts and osteogenic osteosarcoma cells, and immunoelectron-microscopical examination revealed a granular immunoreaction product in the rough endoplasmic reticulum. These findings indicate that FA2 is synthesized by osteoblasts and osteogenic osteosarcoma cells. A reaction of immunological identity was found between FA2 purified from second trimester amniotic fluid and serum-free supernatants of cultured osteogenic osteosarcoma cells. This shows that an antigen recognized by the anti FA2 antibody is secreted by these malignant cells. Thus, FA2 may represent a marker for altered bone metabolism, and have a potential in the classification of osteogenic osteosarcoma/ chondrosarcoma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00174149
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  • 3
    Electronic Resource
    Electronic Resource
    A continuous T-cell line from a patient with Sézary syndrome (1987)
    Springer
    Archives of dermatological research 279 (1987), S. 293-298 
    Details
    ISSN: 1432-069X
    Keywords: T cells ; Sézary syndrome ; Natural killer cells ; Chromosome aberrations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A continuous cell line, Se-Ax, from a patient with Sézary syndrome has been established. The Se-Ax cell line is IL-2 dependent, requires human serum for permanent growth, and has the following phenotype: CD1-, CD2+, CD3+, CD4-, CD5-, CD8-, CD20-, CD25+; it expresses the T9, T10, and HLA-DR antigens. This cell line reveals multiple chromosome aberrations as seen in the most abundant abnormal clone in peripheral blood. Therefore, it is not unlikely that it derives from tumor cells. A putative cytotoxic cell line derived from the same patient has only weak killer-cell activity against the autologous permanent cell line: this CD8+ cytotoxic cell line has a 14q+ chromosomal marker. The fact that the patient demonstrated no natural killer-cell or activated killer-cell activity against the Se-Ax cell line may in part explain the successful establishment of the continuous cell line from bulk culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00431220
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  • 4
    Electronic Resource
    Electronic Resource
    C-type particles are inducible in Se-Ax, a continuous T-cell line from a patient with Sézary's syndrome (1988)
    Springer
    Archives of dermatological research 280 (1988), S. 264-267 
    Details
    ISSN: 1432-069X
    Keywords: C-type particles ; Sézary's syndrome ; T cell ; HTLV-negative ; Retrovirus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Se-Ax is a continuous mature T-cell line that we have established from a patient with Sézary's syndrome. An important finding was that the Se-Ax cell line required human serum for initial growth. Here we show that transfer of the permanent cell line to a medium deficient of human serum induces production of C-type retroviral-like particles with a unique morphology. Ultrastructurally, these particles are 120 nm in diameter with hexagonal shape, and show a small, centrally located round core of 30 nm. They are observed only extracellularly; typical budding, however, is not found. Both by serological testing and molecular analysis we substantiate the morphological conclusion that these particles do not represent common human or animal retroviruses. The inducibility of the particles may be a hint as to a possible endogenous origin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00440598
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  • 5
    Electronic Resource
    Electronic Resource
    Naevus sudoriferus (local eccrine hyperhidrosis) (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of the European Academy of Dermatology and Venereology 9 (1997), S. 0 
    Details
    ISSN: 1468-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Naevus sudoriferus (local hyperhidrosis) or ephidrosis, in an 8-year-old girl is presented. The symptoms consisted of sweating in an area on the dorsal aspect of the right forearm and had been present for approximately 1 year. Episodes of hyperhidrosis occurred 2–4 times daily. The diagnosis was supported using the starch-iodine sweat test, which delineated the area of sweat dysfunction, The patient was treated topically with aluminium chloride solution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1468-3083.1997.tb00518.x
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  • 6
    Electronic Resource
    Electronic Resource
    Crystallization and preliminary investigation of xylose isomerase from Bacillus coagulans (1994)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 50 (1994), S. 231-233 
    Details
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Xylose isomerase from Bacillus coagulans has been crystallized in two different crystal forms. One crystal form is in space group P21212, cell dimensions a = 462, b = 165, c = 82 Å. The other is in space group I422, cell dimensions a = b = 113, c = 153 Å.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0907444993009552
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  • 7
    Electronic Resource
    Electronic Resource
    Human plasminogen binding protein tetranectin: crystallization and preliminary X-ray analysis of the C-type lectin CRD and the full-length protein (1997)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 53 (1997), S. 108-111 
    Details
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: The recombinant human plasminogen binding protein tetranectin (TN) and the C-type lectin CRD of this protein (TN3) have been crystallized. TN3 crystallizes in the tetragonal space group P42212 with cell dimensions a = b = 64.0, c = 75.7 Å and with one molecule per asymmetric unit. The crystals diffract X-rays to at least 2.0 Å resolution. A complete diffraction data set has been collected to 2.7 Å resolution. The crystals of TN, obtained by the vapour-diffusion reverse salting-in method at 280 K, are rhombohedral, space group R3, with the hexagonal axes a = b = 89.1, c = 75.8 Å, and diffract to at least 2.5 Å. A full data set has been collected to 3.0 Å. The asymmetric unit contains one monomer of TN. Molecular replacement solutions for TN3 and TN have been obtained using the structure of the C-type lectin CRD of rat mannose-binding protein as search model. The rhombohedral space group indicates that trimers of TN are formed in accordance with the observation of trimerization in solution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0907444996011493
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  • 8
    Electronic Resource
    Electronic Resource
    Structure determination of glucose isomerase from Streptomyces murinus at 2.6 Å resolution (1994)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 50 (1994), S. 124-131 
    Details
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Glucose isomerase from Streptomyces murinus has been crystallized in space group P41212, cell dimensions a = b = 137.65 and c = 132.20 Å. One dimer of the tetrametric molecule is found per asymmetric unit. An initial structure solution was obtained by the molecular replacement method. The crystallographic refinement was performed using molecular dynamics techniques with X-ray restraints. The final crystallographic R value is 21.4% at 2.6 Å resolution including 3023 non-H atoms, two metal ions and two water molecules per monomer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0907444993009540
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  • 9
    Electronic Resource
    Electronic Resource
    Penicillamine-induced bullous pemphigoid with pemphigus-like antibodies (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of cutaneous pathology 16 (1989), S. 0 
    Details
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A rheumatoid arthritis case is described who developed bullous skin eruption with mucosal involvement after 3 years of treatment with penicillamine. Histologically, changes compatible with bullous pemphigoid were found. Direct immunofluorescence showed linear, as well as intercellular, deposits of IgG. By indirect immunofluorescence IgG antibodies reacting with the basal membrane zone, as well as the intercellular substance, were detected in the serum. We believe that the patient had bullous pemphigoid with pemphigus-like antibodies induced by penicillamine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0560.1989.tb00031.x
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  • 10
    Electronic Resource
    Electronic Resource
    Immunohistochemical studies on the localization of fetal antigen 2 (FA2), laminin, and collagen type 4 in basal cell carcinoma (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of cutaneous pathology 18 (1991), S. 0 
    Details
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The localization of fetal antigen 2 (FA2), a recently described basement membrane (BM) associated antigen, was studied by immunohistochemical techniques in 21 basal cell carcinomas (BCC). In both superficial and infiltrating BCC, FA2 was located in a broad diffuse band around the tumor elements and in close contact with the BM. Compared to normal skin, a more extensive distribution of FA2 was seen in BCC. In the infiltrating BCC, FA2 staining was also present in the interstitial stroma between the tumor islands. FA2 was absent in areas with inflammatory cell infiltrates and elastoid degeneration. Epithelial and tumor cells were FA2 negative. The distribution of FA2 was clearly different from that of laminin and collagen type 4. Collagen type 4 and laminin were present as a continuous linear band corresponding to the BM surrounding the tumors. The close contact to the BM and the increased content of FA2 in the reactive stroma around BCC suggest that FA2 is involved in the matrix and/or BM changes taking place during tumor growth and invasion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0560.1991.tb00156.x
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