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  • 1
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular medicine 46 (1968), S. 44-46 
    ISSN: 1432-1440
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Anti-mouse-thymus globulin (AMT-IgG) prepared from rabbit serum by fractional precipitation with (NH4)2SO4 and diaminoacridin abrogates completely second set reactions against allogeneic skin grafts when injected into A/NMRI mice. Pepsin-digestion of this active globulin yields bivalent antibody fragments (F (ab′)2), which still agglutinate thymocytes in vitro but are no longer effective in prolonging survival of primary allogeneic skin grafts. It is concluded that the immunosuppressive activity of the herologous antilymphocytic globulin is conveyed by theF c fragment. The specific mechanism by which AMT-IgG acts may be complement-dependent, e.g. cytotoxic, or may be induced by cytophilic properties of the antibodies. Injections ofF (ab′) 2 fragments do not produce peripheral lymphopenia.
    Notes: Zusammenfassung Anti-Maus-Thymus-Globulin (AMT-IgG) vom Kaninchen, präparativ durch fraktionierte Fällung mit (NH4)2SO4 und Diaminoacridin hergestellt, unterdrückt die Abstoßung von zweiten allogeneischen Hauttransplantaten (C57/B1/6 auf A/NMRI) in sensibilisierten Mäusen vollständig. Die Verdauung des aktiven IgG-Globulins mit Pepsin ergibt ein bivalentes agglutinierendes Antikörperfragment (F (ab′(2), das keine immunsuppressive Aktivität mehr in vivo besitzt. Die Überlebenszeit primärer Hauttransplantate von C57/B1/6 auf A/NMRI wird durch Injektionen vonF (ab′) 2-Fragmenten des AMT-IgG nicht verlängert. Die mitF (ab′)2 behandelten Mäuse zeigen keine Lymphopenie. Es wird daraus geschlossen, daß ein integresF c -Fragment für die immunsuppressive Wirkung der heterologen Antilymphocyten-Globuline notwendig ist. Die Frage, ob der spezifische Reaktionsmechanismus komplementabhängig ist oder durch die cytophile Aktivität desF c -Fragments bewirkt wird, bleibt offen.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-069X
    Keywords: T-lymphocyte subsets ; Monoclonal antibodies ; Psoriasis vulgaris
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Cryostat tissue sections from skin lesions of 16 patients suffering from chronic stationary psoriasis vulgaris were assayed for the presence of distinct T-cell subpopulations with monoclonal antibodies. Using two pan-T surface markers (M-T 4–11 and Lyt 3) the total number of infiltrating T-cells was measured. This cell population was further dissected into Leu 3a (helper/inducer) and M-T 8–11 (cytotoxic/suppressor) positive subsets. Percentages of T-cell subpopulations were within the ranges found in healthy peripheral blood and were thus regarded as normal.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-069X
    Keywords: Mycosis fungoides ; Histopathology ; Benign inflammatory dermatoses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The cutaneous infiltrate in mycosis fungoides (MF) is predominantly composed of T4-positive T-cells. Attempts to distinguish the early stages of this condition from benign inflammatory infiltrates using anti-T3, T4 and other T-cell-associated antibodies have hitherto been unsucessful. Recently a monoclonal antibody BE 2 has been described as selectively reacting against leukemic cells in patients with cutaneous T-cell lymphoma. To investigate whether the BE 2 antigen is differentially expressed in different stages of MF and benign dermatoses, thus facilitating diagnosis, especially of early MF, the reactivity of monoclonal antibody BE 2 against cutaneous infiltrates from such conditions was assessed. In the early stages of MF only a small number of reactive cells was present. In benign inflammatory infiltrates, especially in those that clinically and histologically were hardly distinguishable from early MF, BE 2 reactivity was essentially the same as in eczematous-stage MF. Lesions from plaque and tumor stage MF contained large numbers of BE 2-reactive cells. Our results indicate that expression of BE 2 is associated with the stage of a given MF lesion and is essentially identical in early MF and eczematous lesions with a similar histopathological appearance.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 681 (1993), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-0584
    Keywords: Hemolysis after BMT
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In ABO mismatched organ or bone marrow transplants recently some cases of acquired immune hemolysis have been reported. It was felt that these life threatening complications were due to immunosuppressive treatment with cyclosporin-A. A case of severe hemolysis following mismatched BMT is reported. Here no cyclosporin-A treatment was given since the bone marrow was T-cell deprived by an E-rosetting technique. Apparently T-cell purging can under these conditions become dangerous.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-0584
    Keywords: Canine lymphocytes ; Monoclonal antibodies ; Mixed lymphocyte culture ; Cell-mediated lympholysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Functional characterization of subsets of T lymphocytes is essential for transplantation studies in dogs, as it is in other species. We studied the function of T cells separated by two mouse monoclonal antibodies recognizing complementary subsets — an antibody directed to canine T cells (MdT-P1) with an up-regulating function, and an antibody directed to human CD 8 (MT811) that cross-reacts with down-regulating canine T cells. Immunorosetting with sheep red blood cells and Percoll gradient allowed us to study depleted and enriched fractions. Their function was tested in mixed lymphocyte culture (MLC), cell-mediated cytotoxicity (CML), and coculture with B cells in a hemolytic plaque assay (PFC). In MLC, MdT-P1-positive cells showed a high proliferative response, and MT811-positive cells responded poorly to allogeneic cells. Vice versa, MT811-negative cells responded strongly, and MdT-P1-negative cells were poor responders but strong stimulators. Effector cells of CML were separated following 8 days of culture and prior to mixing with target cells. Enriched and depleted fractions with either antibody showed low cytotoxic activity as compared with unseparated cells. When added to unseparated effector cells MT811-positive cells suppressed cytotoxicity. B cells were obtained by resetting with staphylococcal protein A (SPA). Their immunoglobulin production was studied following 6 days of culture stimulated by pokeweed mitogen in a reverse hemolytic plaque assay. Again, MT811-positive cells added to the culture suppressed, and MT811-negative cells enhanced immunoglobulin production. In conclusion, immunorosetting with two monoclonal antibodies allowed us to distinguish subpopulations of canine T cells with up-regulating (helper/inducer) from those with down-regulating (suppressor) activity.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-0584
    Keywords: Hematopoietic progenitor cells ; Dog ; Monoclonal antibodies ; CFU-GM ; Autologous bone marrow transplantation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Canine hematopoietic progenitor cells were characterized by separation with monoclonal antibodies. Depleted and enriched fractions were studied for growth of CFU-GM in semisolid agar and for repopulating capacity of lethally irradiated dogs. CFU growth was not reduced by depletion of marrow using monoclonal antibodies F 3-20-7 (anti-dog Thy-1), MT606 (anti-human CD6), and IOT2a (anti-human DR). CFU growth was variable following treatment with the anti-canine T-cell antibody MdT-P1 and immunomagnetic bead separation. It was regularly enriched when MdT-P1 treatment was followed by immunorosetting with staphylococcal protein A-loaded sheep red blood cells and density gradient separation. Lethally irradiated dogs were reconstituted by autologous marrow depleted of MdT-P1-positive cells using immunorosetting and density gradient centrifugation, whereas immunomagnetic bead-depleted marrow was ineffective. Fluorescence-activated cell sorting showed enrichment of hematopoietic progenitor cells in the weakly MdT-P1-positive fraction.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Archives of dermatological research 277 (1985), S. 444-447 
    ISSN: 1432-069X
    Keywords: HLA-DR expression ; Keratinocytes ; Lupus erythematosus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary It has been suggested that aberrant expression of HLA-DR plays an important role in the induction of an autoimmune reaction. In lupus erythematosus (LE), skin represents a major target for autoimmune attack. We therefore tested lesional and nonlesional skin from 11 patients with different clinical subtypes of LE for the presence of HLA-DR molecules on keratinocytes using a monoclonal antibody against a non-polymorphic HLA-DR determinant. In all lesions tested, HLA-DR-positive keratinocytes were present, whereas in nonlesional skin, these cells remained HLA-DR negative. To exclude the possibility of passive absorption of DR molecules, the de novo synthesis of HLA-DR was demonstrated by the presence of cytoplasmic HLA-DR γ chains in keratinocytes. If aberrant HLA-DR expression is a primary event, it could facilitate the recognition of autoantigens on keratinocytes by immunocompetent cells. Alternatively, the synthesis of DR molecules could be induced as a secondary event by mediators derived from the inflammatory infiltrate.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1437-160X
    Keywords: Rheumatic diseases ; OKT markers ; ANAE staining ; Synovial fluid ; Blood
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Mononuclear cell preparations from peripheral blood (PBL) and synovial fluid (SFL) of 27 Patients with rheumatoid diseases (15 patients with definite rheumatoid arthritis (RA), 10 with other inflammatory joint diseases (OJD), 1 with sarcoid arthritis (SA) and 1 with traumatic arthritis (TA) were examined for lymphocyte subpopulations determined by monoclonal antibodies of the OKT series and by the dot-like, acid α-naphthyl esterase staining (ANAE) activity. In patients with classic, active RA, blood T cells carrying the OKT8+ (suppressor/killer) phenotype were significantly reduced leading to an elevated OKT4/OKT8 ratio of 4.1±0.4 compared with 2.1±0.1 in healthy controls. In 10 patients with OJD this diminution of OKT8+ cells in peripheral blood was less pronounced or absent. As regards SFL subpopulations, patients with RA and OJD exhibited a similar distribution pattern with an elevation of OKT8+, Ia+ and ANAE negative cells and a similar OKT4/OKT8 ratio of 1.5±0.3 and 1.6±0.4, respectively. Similar results were also obtained in the only patient with TA, whereas the patient with SA and one RA patient with relapse after surgical synovectomy exhibited high OKT4/OKT8 ratios, both in synovial fluid and peripheral blood. Neither the OKT markers nor the dot-like ANAE staining pattern were significantly correlated to parameters of systemic or local disease activity as estimated by erythrocyte sedimentation rate and a local disease activity index.
    Type of Medium: Electronic Resource
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