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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Inorganic chemistry 14 (1975), S. 2537-2540 
    ISSN: 1520-510X
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Inorganic chemistry 16 (1977), S. 2735-2742 
    ISSN: 1520-510X
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1520-510X
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Biochemistry 11 (1972), S. 434-438 
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Marine biology 101 (1989), S. 451-456 
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Selected features of Alaria marginata Postels et Ruprecht, Laminaria saccharina (L.) Lamouroux and Cymathere triplicata (Postels et Ruprecht) J. G. Agardh meiospores were described using flow cytometry. The relative sizes (forward scatter), chlorophyll contents (red fluorescence) and DNA contents (blue fluorescence) were measured on living, fixed, Hoechst and DAPI (4′,6-diamidino-2-phenylindole) stained and unstained cells. The meiospores were similar among species and the frequency distributions of the monitored features were essentially normal. Meiospores sorted for low blue fluorescence departed significantly from the expected 1:1 male to female ratio in favour of the male. Cells sorted for high blue fluorescence did not result in a departure from the expected 1:1 ratio. We suggest that our ability to sexually sort meiospores is a result of a differential nonstoichiometric fluorescence in DNA, which possibly reflects a greater DNA compactedness and/or nuclear protein content in the male meiospores.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Microbial ecology 29 (1995), S. 71-81 
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The occurrence and distribution of Azorhizobium and Rhizobium strains that induce stem nodulation of Sesbania rostrata were determined in four vegetation zones in Senegal. Based on tests with 16 Rhizobium and 10 Azorhizobium strains nodulating S. rostrata, a method was devised to distinguish among the strains. In all vegetation zones, members of both genera were more abundant in rhizosphere than nonrhizosphere soil under S. rostrata, Cassia obtusifolia, Acacia senegal, and Hystic suaveolens, and Rhizobium was present at higher densities than Azorhizobium. Azorhizobium was more abundant on the leaves and stems than Rhizobium in three of the vegetation zones, and the density of Azorhizobium but not Rhizobium was far greater on the leaves of S. rostrata than the three nonhost species in all four zones. Approximately 90% of the stem nodules and 39–48% of the root nodules on S. rostrata in all four zones were formed by Azorhizobium.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Analysis of continuous culture methodology suggests that this potentially powerful tool for kinetic analysis can be improved by minimizing several inherent shortcomings. Medium background substrates — organic carbon, phosphate, and manganese — were shown to dominate kinetic observations at concentrations below chemical detection methods. Reactor wall growth, culture size distribution changes, sample removal-induced steady state perturbations, and limiting substrate leakage from organisms are treated in terms of kinetic measurement errors. Large variations in maximal growth rates and substrate uptake rates found are attributed to experimental protocol-induced transient states. Relationships are presented for correcting limiting substrate concentrations for lability during sampling, contamination with unreacted medium, and background substrate effects. Analytical procedures are discussed for improved measurement of limiting substrate kinetics involving enzymes, isotopes, and material balance manipulation. Relaxation methods as applied to continuous culture are introduced as a means for isolating separate rate constants describing net substrate transport and for evaluating cellular metabolite leakage. Low velocity growth, multiple substrate metabolism, and endogenous metabolism are discussed along with measurements showing that 1-month generation times for aquatic microorganisms can be quite normal and that the kinetics are compatible withμg/liter limiting substrate concentrations. The concept of regarding growth kinetics as the sum of several net accumulation processes is suggested.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-1106
    Keywords: Anterograde transport ; Wheat germ agglutinin conjugated horseradish peroxidase ; Cerebellum ; Ascending spinal tracts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Wheat germ agglutinin-horseradish peroxidase (WGA-HRP) conjugate was injected into the lumbar or cervical spinal cord of adult cats in order to examine its usefulness as an anterograde tracer of long ascending spinal tracts. Heavily labeled fibers and terminal-like structures were found in restricted regions of the granular layer of the cerebellar cortex in all animals. The terminal-like structures showed features characteristic of mossy fiber rosettes. Many labeled terminals were found in regions anatomically and physiologically defined as cerebellar hind limb regions after lumbar cord injections. Relatively few labeled terminals were found in both hind limb and in forelimb regions after cervical cord injections. Although the cervical spinal cord is known to project to both hind limb and forelimb regions, it cannot be entirely excluded that some of the labeling in the hind limb regions was derived from uptake by fibers of passage. In addition to labeling in the cerebellar cortex, labeled fibers and terminals were also observed in the cerebellar medial and interposed nuclei, regardless of whether the injections had been made into the lumbar or the cervical spinal cord. Several midbrain and thalamic regions were found to contain labeled fibers and terminal-like profiles in all cases. In the midbrain the periaqueductal gray, the nucleus of Darkschewitsch and the posterior pretectal nucleus contained fairly dense labeling. Labeling was more scattered in the cuneiform nucleus, the mesencephalic reticular formation, the superior colliculus and in the magnocellular part of the medial geniculate body. Four major regions in the thalamus, namely the central lateral nucleus, the zona incerta, the medial part of the posterior complex and the submedius nucleus contained labeling of variable density. The present findings show that the WGA-HRP conjugate can be used as a tracer for the study of long fiber tracts within the central nervous system.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1432-1106
    Keywords: Dorsal root ganglion ; Nucleus gracilis ; Sciatic nerve ; Nerve injury ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Rat lumbar dorsal root ganglion neurones projecting to the nucleus gracilis in the brainstem were retrogradely labelled with Fluoro-Gold and analysed immunocytochemically for their expression of substance P-, calcitonin gene-related peptide-, galanin-, galanin message-associated peptide-, neuropeptide Y-, nitric oxide synthase- and carbonic anhydrase-like immunoreactivity as well as affinity to Griffonia (bandeiraea) simplicifolia lectin I — isolectin B4, RT97 and to choleragenoid. The analysis was made both in uninjured rats and in rats which had been subjected to unilateral sciatic nerve transection and partial resection 3 weeks earlier. The data showed that 6% of the L4 and L5 lumbar dorsal root ganglion cells that projected to the nucleus gracilis showed substance P-like immunoreactivity. Following nerve injury, none of the nucleus gracilis-projecting dorsal root ganglion cells showed substance P-like immunoreactivity. Nineteen per cent of the investigated cell population showed calcitonin gene-related peptide-like immunoreactivity in uninjured rats, but no nucleus gracilisprojecting calcitonin gene-related peptide-positive cells were found after nerve injury. Galanin- and galanin message-associated peptide-like immunoreactivity were found in 2% and 3%, respectively, of the Fluoro-Gold-labelled cell population normally and in 22% and 14%, respectively, after injury. No neuropeptide Y-positive cells were found in the Fluoro-Gold-labelled cell population normally, but after nerve injury, 96% of this population became neuropeptide Y-positive. Nitric oxide synthaselike immunoreactivity was found in 2% of the Fluoro-Gold-labelled cells normally and in 10% after injury. Two per cent of the Fluoro-Gold-labelled cells in the normal cases were stained by Griffonia (bandeiraea) simplicifolia lectin I — isolectin B4. After injury, however, no such double labelling was found. Thirty-four per cent of the Fluoro-Gold-labelled cell population was carbonic anhydrase positive normally, and 42% after injury. Seventy-five per cent of the Fluoro-Gold-labelled cells showed RT97 immunoreactivity normally and 12% after injury. Choleragenoid-like immunoreactivity was found in 99% of the Fluoro-Gold-labelled dorsal root ganglion cells normally and 81% after injury. Immunohistochemical visualisation of choleragenoid transganglionically transported from the injured sciatic nerve combined with neuropeptide Y immunocytochemistry showed that primary afferent fibres and terminals in the nucleus gracilis contain neuropeptide Y following peripheral nerve transection. Taken together, the results indicate that peripherally axotomised nucleus gracilis-projecting neurones undergo marked alterations in their cytochemical characteristics, which may be significant for the structural and functional plasticity of this system after injury.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    European journal of pediatrics 136 (1981), S. 173-179 
    ISSN: 1432-1076
    Keywords: Lung surfactant ; Surfactant substitution ; Lung mechanics ; First breath ; Artificial ventilation ; Premature newborn rabbit ; Experimental hyaline membrane disease ; Bronchiolar epithelial lesions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Premature newborn rabbits, delivered on day 27 of gestation, were subjected to positive-pressure ventilation, with or without treatment with natural surfactant. The surfactant, obtained by centrifugation of lung wash from adult rabbits, was deposited in the tracheal cannula before the onset of ventilation. Parameters of lung mechanics, recorded during spontaneous ventilation after 1 h, were significantly improved in animals receiving surfactant. In comparison with littermate controls, surfactant-treated animals also had less prominent bronchiolar epithelial lesions. We conclude that treatment with supplementary surfactant facilitates functional adaptation of the premature lung and prevents the development of epithelial lung lesions during artificial ventilation.
    Type of Medium: Electronic Resource
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