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  • 1
    ISSN: 1432-0568
    Keywords: Plasminogen activator ; Plasminogen activator inhibitor ; Bone formation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The role of urokinase plasminogen activator and plasminogen activator inhibitor-1 in human embryofetal bone formation between the 9th and the 20th week of gestation has been studied immunohistochemically. While mature osteocytes of the secondary spongiosa and resting chondrocytes of the bone epiphyses were negative for both antigens in each developmental stage, metabolically active parts of the osseocartilaginous system showed a strong immunoreactivity. Until the end of the 10th week of gestation urokinase plasminogen activator and plasminogen activator inhibitor-1 could not be demonstrated in the shaft of the preexisting cartilaginous models of bones, which correlates with the morphological developmental stage of the embryos. Later, osteoblasts and chondrocytes in the areas of enchondral ossification, and the perivascular chondrocytes of the epiphyseal secondary ossification centres, showed similarly high concentrations of urokinase plasminogen activator and plasminogen activator inhibitor-1. Moreover, the individual ossification stages of the different bones in embryo-fetal development could be demonstrated immunohistochemically. While humeri and femora showed diaphyseal immunoreactivities at an early stage, positive reactions in the phalanges were found only much later. Thus, the enzymes of the fibrinolytic system studied are clearly involved in the desmal and enchondral ossification process in the osseocartilaginous compartment.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular medicine 54 (1976), S. 1107-1108 
    ISSN: 1432-1440
    Keywords: Extrahepatic obstruction ; Mitochondrial morphology ; Cholestasis ; Glutamate-Dehydrogenase ; Extrahepatischer Verschluß ; Mitochondrienmorphologie ; Cholestase ; Glutamatdehydrogenase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Als feinstrukturell-morphometrisches Korrelat der bei extrahepatischer Cholestase beobachteten Aktivitätserhöhung der GLDH im Serum ließ sich eine Schwellung der Mitochondrien in der Leber nachweisen. Die morphometrischen Veränderungen gehen mit den serochemischen Befunden parallel: zwischen Mitochondrienschwellung und GLDH-Aktivität im Serum besteht eine signifikante Korrelation.
    Notes: Summary Corresponding to the increased activity of mitochondrial Glutamate-Dehydrogenase (GLDH) in serum in extrahepatic cholestasis a swelling of hepatic mitochondria could be shown on ultrastructural morphometric analysis. The morphometric alterations parallel the serochemical findings. A significant correlation could be calculated between the swelling of mitochondria and the activity of GLDH in serum.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 598 (1990), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1365-2559
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 0165-4608
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Journal of Ultrasructure Research 45 (1973), S. 356-365 
    ISSN: 0022-5320
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-2307
    Keywords: Multidrug resistance ; P-glycoprotein Neuroblastoma ; Nephroblastoma ; Reverse transcriptase polymerase chain reaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We evaluated the expression of MDR1/p-glycoprotein in paediatric tumours using reverse transcriptase polymerase chain reaction (RT-PCR), RNA dot blot analysis, and immunohistochemistry on formalin fixed paraffin-embedded material with JSB-1 and C-219 monoclonal antibodies, and compared these three techniques. The expression of multidrug resistance-associated protein (MRP) gene was examined by RT-PCR assay. We studied MDR1/p-glycoprotein and MRP expression in 13 samples from 10 neuroblastoma patients, 11 samples from 10 nephroblastoma patients, 2 rhabdomyosarcomas, 1 adrenocortical carcinoma and 10 benign tumours or tumour-like lesions. Eleven of 13 neuroblastomas, 7 of 11 nephroblastomas, 2 rhabdomyosarcomas, 1 adrenocortical carcinoma, and 7 of 10 benign tumours or tumour-like lesions showed MDR1 PCR products. By RNA dot blot analysis, MDR1 transcripts were detectable in 11 of 34 specimens. Immunohistochemically, we detected positive reaction products for JSB-1 in 26 of 36 samples. There was a significant correlation between the immunoreactivity for JSB-1 and the expression of MDR1 mRNA expression by RTPCR (P=0.0001). However, the presence of p-glycoprotein immunostaining does not correlate with the MDR1 expression shown by RT-PCR in every case. As for MRP mRNA expression, 9 of 13 neuroblastomas and 10 of 11 nephroblastomas revealed PCR products.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-2307
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Lymphocytes and plasma cells have been demonstrated in the peripheral zones of lobules in liver biopsies from patients suffering from chronic aggressive hepatitis. In the region of contact between lymphocytes and parenchymal cells, the cell membranes are frequently not demonstrable so that an impression is created of cytoplasmic fusion. However, when the goniometer is used, the cell membranes in this region can still be demonstrated at a certain tilt angle. It can therefore be concluded that the presumed cytoplasmic fusions are frequently the result of superposition effects in projection.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1432-2307
    Keywords: Human atherosclerotic plaque ; Phenotypic characterization of cell types ; Monoclonal antibodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Sections of human atherosclerotic plaques, obtained from 21 autopsy cases with various degrees of atherosclerosis, were stained with the indirect immunoperoxidase technique using specific monoclonal antibodies against macrophages and smooth muscle cells. Distinctive results were found in differing stages: Single blood monocytes were observed in diffuse intimal thickening and the foam cells seen in fatty streaks were mostly identified as mature tissue macrophages, while only very few blood monocytes were present. The spindle cells observed in fibroelastic plaques showed positive reactions to antibodies against desmin, which points to their derivation from smooth muscle cells, whereas only a few macrophage-derived foam cells were seen in these lesions. In the complicated lesions the majority of foam cells were macrophage-derived, but there was also a small number of foam cells positive to antibodies against desmin, suggesting a smooth muscle cell derivation. - Our results confirm that in human atherosclerotic plaques the majority of the foam cells are obviously macrophage-derived, which emphasizes the important role of macrophages in the morphogenesis of these lesions.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-2307
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung 40 Leberbiopsien von Patienten mit klinisch gesicherter intrahepatischer Cholestase wurden feinstrukturell-morphometrisch ausgewertet. Zusätzlich wurden im Serum der Patienten die Aktivitäten der „Cholestase anzeigenden Enzyme” bestimmt und mit den morphometrischen Parametern der Gallencapillaren statistisch korreliert. Es konnte so nachgewiesen werden, daß zwischen den Serumaktivitäten der Leucinaminopeptidase und der 5′-Nucleotidase einerseits und der Morphologie der Gallencapillaren andererseits statistisch gesicherte Korrelationen bestehen. Bei einer Erhöhung der Serumaktivität der Leucinaminopeptidase fand sich eine signifikante Erweiterung des Volumens der Gallencapillaren, bei erhöhter 5′-Nucleotidase im Serum war dagegen die Anzahl der Gallencapillarenanschnitte im elektronenmikroskopischen Bild signifikant erhöht. Auch bei vermehrter Gallengangsphosphatase war das Volumen der Gallencapillaren vergrößert, diese Korrelation konnte jedoch statistisch nicht gesichert werden. Für die alkalische Gesamtphosphatase und das Gesamtbilirubin ließen sich in den untersuchten Fällen keine Beziehungen zur Morphologie der Gallencapillaren nachweisen. Die erhobenen Befunde zeigen, daß bei intrahepatischer Cholestase der feinstrukturelle Leberbefund und das Enzymmuster der Cholestaseenzyme im Serum miteinander in Beziehung stehen und sich bei der Beurteilung des Krankheitsbildes ergänzen.
    Notes: Summary Reports on correlations between the activity of so-called “marker enzymes of cholestasis” in serum and the ultrastructural changes of the liver are rare. Therefore studies of ultrastructural changes were carried out in 40 patients with intrahepatic cholestasis. In the patients' serum the activity of alkaline phosphatase, bile duct alkaline phosphatase, leucine-aminopeptidase (LAP), and 5′-nucleotidase (5′-Nu) as well as the concentration of bilirubin were determined. The results showed a significant correlation between the morphometry of the bile canaliculi and the serum activity of LAP and 5′-Nu. In patients with elevated LAP, an enlargement of the bile canaliculi could be proved. An increased serum activity of 5′-Nu correlated with a higher incidence of bile canaliculi in the ultrastructural picture. The results suggest that investigation of the ultrastructure of bile canaliculi and the determination of marker enzymes of cholestasis in the serum may both contribute to the assessment of cholestatic liver disease.
    Type of Medium: Electronic Resource
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