ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
The C-terminal two-thirds segment of integrase derived from the simian immunodeficiency virus has been cloned, expressed in Escherichia coli, and purified to greater than 95% homogeneity. The protein encompasses amino-acid residues 50–293 and contains a F185H substitution to enhance solubility. In dilute solutions at concentrations below 1 mg ml−1, the enzyme is predominantly dimeric. At the higher concentrations (〉10 mg ml−1) required to enable crystallization, the enzyme self-associates to form species with molecular weights greater than 200 kDa. Despite the apparent high aggregation in solution, the enzyme crystallizes from a 8%(v/v) polyethylene glycol (molecular weight 6000) solution in a form suitable for X-ray diffraction studies. The resulting single crystals belong to the space group P212121, with unit-cell parameters a = 79.76, b = 99.98, c = 150.2 Å, α = β = γ = 90° and Z = 4. Under X-ray irradiation generated with a rotating-anode generator, the crystals diffract to 2.8 Å resolution and allow collection of a native 3 Å resolution diffraction data set.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444999009610
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