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  • 1
    ISSN: 1432-1076
    Keywords: Key words HIV-1 ; T-cells ; CD69 ; Activation ; Children
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We evaluated the use of a whole-blood assay that measures spontaneous and activation-induced CD69 expression on peripheral blood T-cells in vitro for assessment of T-cell function in HIV-1-infected paediatric patients. Heparinized venous blood from 28 HIV-1 positive children and adolescents and 23 healthy controls was incubated for 4 h with or without 5 μg/ml phytohaemagglutinin (PHA). Thereafter, analysis of CD69 expression on CD4+ and CD8+ T-cells was done by flow cytometry; simultaneously we determined CD4+ T-cell counts and plasma HIV-1 viral load. Neither spontaneous nor PHA-induced CD69 expression differed significantly between HIV-1 positive patients and healthy controls. However, T-cells from HIV-1 positive patients with plasma HIV-1 viral load levels above 70 × 103 copies/ml showed a higher spontaneous CD69 expression than T-cells from patients with lower plasma viral load levels in different stages of the disease. Antiretroviral treatment in four patients reduced spontaneous CD69 expression in CD4+ T-cells and PHA-induced CD69 expression in CD4+ and CD8+ T-cells significantly after 8 weeks of therapy. Conclusion Spontaneous and activation-induced expression of the early (activation) antigen CD69 on peripheral blood T-cells does not distinguish HIV-1 positive patients from HIV-1 negative healthy controls and is not correlated with peripheral blood CD4+ T-cell counts. This test may not be a reliable marker for functional T-cell deficiency during early stages of HIV disease. Increased spontaneous as well as PHA-induced CD69 expression on T-cells from HIV-1-infected children and adolescents in vitro may rather reflect HIV-induced pre-activation of T-cells in vivo.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1173
    Keywords: Schlüsselwörter Akroangiodermatitis ; Morbus Mali ; Stewart-Bluefarb-Syndrom ; Pseudo-Kaposi-Sarkom ; Kaposi-Sarkom ; Key words Acroangiodermatitis ; Morbus Mali ; Stewart-Bluefarb-syndrome ; Pseudo-Kaposi’s-sarcoma ; Kaposi’s sarcoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary A 76-year-old female patient developed severe manifestations of a kaposi-like acroangiodermatitis (so-called Mali’s disease) due to chronic venous insufficiency of the lower extremities. The patient presented with large areas of confluent, violaceous or brown-black papules on both lower legs. Histologically, proliferation of thick-walled capillaries was seen in the upper dermis consisting of fully differentiated endothelial cells, as shown by immunohistochemistry. In contrast to true Kaposi’s sarcoma, human-herpes-virus-8 DNA could not be detected by polymerase-chain-reaction in this condition. We review the diagnostic criteria used to distinguish between acroangiodermatitis, also called pseudo-Kaposi’s sarcoma, and the true Kaposi’s sarcoma.
    Notes: Zusammenfassung Wir berichten über eine 76jährige Patientin mit einer ausgeprägten kaposiformen Akroangiodermatitis (Morbus Mali), die sich klinisch mit großflächig konfluierenden, lividroten bis bläulich-schwarzen Papeln an beiden Unterschenkeln manifestierte und auf einer chronisch-venösen Insuffizienz Grad II nach Widmer mit Stammvarikosis Grad IV nach Hach sowie Parva- und Perforansinsuffizienzen bereits beruhte. Histologisch fanden sich, beschränkt auf die obere Dermis, prominente, dickwandige Kapillarproliferate, deren Endothelzellen immunhistologisch als ausgereift identifiziert werden konnten. In der Polymerase-Ketten-Reaktion war das Humane Herpes-Virus-8, das neuerdings beim klassischen und HIV-assoziierten, echten Kaposi-Sarkom nachgewiesen wurde, nicht nachweisbar. Das Krankheitsbild war bei unserer Patientin so ausgeprägt, daß wir hier unter Berücksichtigung der neueren Literatur erneut auf die Kriterien zur Abgrenzung der kaposiformen Akroangiodermatitis vom Kaposi-Sarkom eingehen.
    Type of Medium: Electronic Resource
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