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  • 1
    Electronic Resource
    Electronic Resource
    Supercritical fluid extraction of moist snuff (1991)
    s.l. : American Chemical Society
    Journal of agricultural and food chemistry 39 (1991), S. 508-510 
    Details
    ISSN: 1520-5118
    Source: ACS Legacy Archives
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/jf00003a015
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  • 2
    Electronic Resource
    Electronic Resource
    Chemical Investigation and Anti-Inflammatory Activity of Vitex negundo Seeds (1992)
    s.l. : American Chemical Society
    Journal of natural products 55 (1992), S. 163-167 
    Details
    ISSN: 1520-6025
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/np50080a002
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  • 3
    Electronic Resource
    Electronic Resource
    On the interchromosomal connections in plants (1984)
    Springer
    Cellular and molecular life sciences 40 (1984), S. 94-95 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using Giemsa C-banding technique, the existence of interchromosomal connections in well spread root tip metaphase plates in a variety of plant species has been demonstrated. Various types of interchromosomal connections are observed, involving satellite, telomeres, interstitial regions and in a few cases centromeres, too. The possible role of these interchromosomal connections in establishing the homologous and non-homologous association in the somatic chromosomes and maintaining the spatial relationship of the genetic apparatus is indicated. In the majority of the cases the connections are made up of constitutive heterochromatin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01959121
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  • 4
    Electronic Resource
    Electronic Resource
    Microangiopathy in human diabetic neuropathy: relationship between capillary abnormalities and the severity of neuropathy (1989)
    Springer
    Diabetologia 32 (1989), S. 92-102 
    Details
    ISSN: 1432-0428
    Keywords: Diabetes ; neuropathy ; microangiopathy ; heterogeneity ; morphometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Clinical, electrophysiological and ultrastractural morphometric observations were made in 5 diabetic non-neuropathic patients, 5 diabetic patients with mild neuropathy and 11 diabetic patients with severe neuropathy. Capillary abnormalities were assessed in simultaneous nerve, muscle and skin biopsies and compared with results from 6 age-matched, non-diabetic control subjects. Nerve capillaries demonstrated markedly greater pathology than skin and muscle capillaries. Endoneurial capillary density was significantly reduced in severely neuropathic diabetic patients (p〈0.01) when compared with control subjects. Capillary basement membrane (p〈0.002), endothelial cell (p〈0.003) and total diffusion barrier (endothelial cell, pericyte, basement membrane) (p〈0.001) thickness were significantly increased, and oxygen diffusing capacity was significantly reduced (p〈0.001) in the nerves of patients with severe diabetic neuropathy when compared to control subjects. Endothelial cell profile number and luminal perimeter were significantly increased in asymptomatic (p〈0.01), (p〈0.05) and severely neuropathic (p〈0.001), (p〈0.05) diabetic patients respectively. However, endothelial cell outer perimeter, a measure of capillary size, showed no significant increase in diabetic patients when compared with control subjects. An association was observed between neurophysiological and neuropathological measures of neuropathic severity. There was no significant correlation between the duration of diabetes and HbA1 levels with capillary pathology or with neuropathic severity. Very few abnormalities of muscle and skin correlated with neuropathic severity. However, all measures of nerve capillary pathology correlated significantly with neurophysiological and neuropathological measures of neuropathic severity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00505180
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  • 5
    Electronic Resource
    Electronic Resource
    Endoneurial localisation of microvascular damage in human diabetic neuropathy (1993)
    Springer
    Diabetologia 36 (1993), S. 454-459 
    Details
    ISSN: 1432-0428
    Keywords: Diabetes mellitus ; microangiopathy ; peripheral neuropathy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Twenty diabetic patients with neuropathy underwent clinical and neurophysiological evaluation together with a detailed morphometric assessment of capillary pathology in endoneurial and epineurial microvascular beds of the sural nerve. Morphological data were compared with ten non-diabetic control subjects. There were no significant differences in control subjects between basement membrane area, endothelial cell area, endothelial cell profile number or luminal area of endoneurial when compared with epineurial capillaries. In contrast, when compared with epineurial capillaries, endoneurial capillaries from diabetic patients demonstrated a significant increase in basement membrane (p〈0.001) and endothelial cell (p〈0.001) area and a significant reduction in luminal area (p〈0.001). There was no significant difference in endothelial cell profile number between endoneurial and epineurial capillaries amongst diabetic patients. Previous studies have demonstrated a good correlation between the degree of microangiopathy and measures of neuropathic severity. In the present study increased endoneurial capillary basement membrane area was significantly related to reduced peroneal nerve conduction velocity (p〈0.001), myelinated fibre density (p〈0.001) and elevated vibration (p〈0.05) and thermal (p〈0.001) perception. Increased endothelial cell area and reduced luminal size were related to a reduced peroneal nerve conduction (p〈0.05, p〈0.01, respectively), reduced myelinated fibre density (p〈0.05, p〈0.01) and elevated thermal perception (p〈0.05, p〈0.001). Epineurial capillary basement membrane, endothelial cell and luminal area failed to relate to measures of neuropathic severity. This study has demonstrated more advanced microangiopathy and a more significant relationship to neuropathic severity in endoneurial compared with epineurial capillaries, thus providing further support for the role of microangiopathy in the pathogenesis of human diabetic neuropathy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00402283
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  • 6
    Electronic Resource
    Electronic Resource
    Changes with age in the peripheral nerves of the rat (1980)
    Springer
    Acta neuropathologica 52 (1980), S. 1-6 
    Details
    ISSN: 1432-0533
    Keywords: Peripheral nerves ; Aging ; Pressure neuropathy ; Axonal glycogen bodies ; Polyglucosan bodies ; Hirano bodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Ultrastructural observations have been made on the tibial and plantar nerves of Wistar rats aged 18–24 months. Changes indicative of segmental demyelination and remyelination and axonal degeneration and regeneration were prominent in the plantar nerves. Both in the plantar and tibial nerves, but particularly in the former, axonal abnormalities were frequent. These included the occurrence of multiple intra-axonal vacuoles containing glycogen and polyglucosan bodies. Axonal sequestration by adaxonal Schwann cell processes was also increased. The Schwann cell cytoplasm in relation to this activity contained bundles of filaments with the ultrastructural features of Hirano bodies. The changes in the plantar nerves probably indicate a pressure neuropathy, but the possibility of a superimposed distal axonal degeneration related to aging cannot be excluded on the present evidence. Such changes must be taken into consideration in experimental studies performed on rats of this age.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00687222
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  • 7
    Electronic Resource
    Electronic Resource
    Influence of streptozotocin-induced diabetes on myelinated nerve fibre maturation and on body growth in the rat (1981)
    Springer
    Acta neuropathologica 53 (1981), S. 257-265 
    Details
    ISSN: 1432-0533
    Keywords: Experimental diabetes ; Skeletal growth ; Nerve fibre maturation ; Diabetic neuropathy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Observations were made between the ages of 2 and 12 months on rats made diabetic with streptozotocin at the age of 1 month, and compared with the findings in age-matched controls. Tibial length and body weight in the control animals increased progressively over the period examined, the growth rate being more rapid in the initial stages. Both of these parameters were consistently less in the diabetic animals over the whole of the observation period. Myelinated fibre numbers and diameters were measured in the tibial and plantar nerves. In the tibial nerve, fibre diameter did not differ between the diabetic and control animals up until 4 months of age; thereafter it changed little in the diabetic animals, but continued to increase in the controls. The findings in the medial plantar nerve were more difficult to analyse but showed comparable although less pronounced changes; fibre diameter may be have diminished in the diabetic nerves after 6 months. Teased fibre studies demonstrated few abnormalities in the tibial nerve, either in the control or the diabetic rats. In the lateral plantar nerves, there was a significant excess of axonal degeneration and regeneration in the diabetic nerves. It was concluded that diabetes impairs growth in nerve fibre diameter, but only after 4 months of age. Before then, no growth retardation is obvious, despite the fact that tibial length and body weight are less. This suggests that the peripheral nervous system may be protected against growth retardation during the early part of the postnatal growth period. The significance of the axonal degeneration in the plantar nerves is uncertain, but it may represent either an increased vulnerability of diabetic nerve to compression injury or, less probably, a distal axonopathy related to the diabetic state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690367
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  • 8
    Electronic Resource
    Electronic Resource
    Effect of an AM fungal consortium and Pseudomonas on the growth and nutrient uptake of Eucalyptus hybrid (2000)
    Springer
    Mycorrhiza 10 (2000), S. 55-61 
    Details
    ISSN: 1432-1890
    Keywords: Key words Eucalyptus ; PRS9 ; Fluorescent ; pseudomonad ; AM fungi ; Interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Eucalyptus is an important tree species used for afforestation of large tracts of marginal and wastelands. Eucalyptus-arbuscular mycorrhizal fungal (AMF) interactions in seedling establishment and growth promotion have been inadequately dealt with. Efforts were made to assess the role of AMF-pseudomonad (PRS9, plant growth promotory fluorescent Pseudomonas) interactions in growth promotion and nursery establishment of E. hybrid. Seedlings were subjected to six different treatments: (i) uninoculated control, (ii) 400 AM spores, (iii) 800 AMF spores, (iv) PRS9 (v) 400 AMF spores + PRS9, (vi) 800 AMF spores + PRS9, with the different P regimes of 10, 20 and 30 ppm. Root length, shoot length, root and shoot fresh and dry weights were maximal at 400 AMF spores and 20 ppm soil P. Shoot P content was maximal at 800 AMF spores followed by 400 AMF spores and 400 AMF spores + PRS9. In general, plant growth was greater at 20 ppm P. Root P content increased significantly with 400 AMF spores followed by 800 at 20 ppm P. Independent of soil P levels, the quality index of mycorrhizal treatments without PRS9 was significantly higher than the treatments including PRS9 or PRS9 alone. Mycorrhizal inoculation efficiency was superior at 10 ppm P irrespective of the treatment. AM alone (400 spores) significantly improved the inoculation efficiency. PRS9 in association with AM fungi inhibited growth promotion and nutrient uptake
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s005720000057
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  • 9
    Electronic Resource
    Electronic Resource
    Effect of vesicular-arbuscular mycorrhizae and zinc application on dry matter and zinc uptake of greengram (Vigna radiata L. Wilczek) (1991)
    Springer
    Biology and fertility of soils 11 (1991), S. 52-56 
    Details
    ISSN: 1432-0789
    Keywords: Vesicular-arbuscular mycorrhizae ; Inoculation ; Zn application ; Zn uptake ; Greengram ; Vigna radiata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In a greenhouse study we examined the effects of vesicular-arbuscular mycorrhizae (VAM) inoculation, using Glomus macrocarpum and of Zn application on dry matter production and Zn uptake by greengram in two mollisols. The VAM inoculation significantly increased the dry weight of different plant parts and the Zn uptake in both soils. Inoculated plants showed a greater response to the application of Zn at 2.5 and 5.0 mg kg-1 soil in a Zn deficient clay loam soil. The inoculated plants also absorbed — more water than the uninoculated plants. Mass flow and diffusion were the principal processes by which Zn reached the plant roots; mass flow was particularly important in the absence of VAM in a sandy soil fertilized with higher Zn doses (5 and 10 mg kg-1 soil). The greater supply of Zn to inoculated roots was attributed to an apparent diffusion process rather than to mass flow of Zn.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00335835
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  • 10
    Electronic Resource
    Electronic Resource
    Purification, crystallization and preliminary crystallographic analysis of mare lactoferrin (1996)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 52 (1996), S. 1196-1198 
    Details
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Lactoferrin is an iron-binding glycoprotein with a molecular weight of 80 kDa. The protein has two iron binding sites. It has two structural lobes, each housing one Fe3+ and the synergistic CO32− ion. The protein was isolated from the colostrum/milk of mares maintained at National Research Centre on Equines, Hisar, India. The purified samples of the protein were crystallized using a microdialysis method. The protein was dialysed against low ionic strength buffer solution. Several crystal forms were obtained, out of which three were characterized which have cell dimensions as follows. Form I a = 79.8, b = 103.5, c = 112.0 Å, space group P212121, with one protein molecule per asymmetric unit and a solvent content of 57%. Form II a = 84.9, b = 99.7, c = 103.5 Å, space group P212121 with one molecule per asymmetric unit and a solvent content of 55%. Form III a = 151.0, b = 151.0, c = 240.6 Å, space group P41212 with three molecules in the asymmetric unit and a solvent content of 57%. The intensity data up to 3.8 Å resolution for form I, 2.9 Å resolution data for form II and 6 Å resolution data for form III have been collected. Further calculations are in progress.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0907444996007986
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