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  • 1
    Electronic Resource
    Electronic Resource
    Required ultracentrifugal time for condensing solution by ultracentrifuge (1990)
    Springer
    Bioprocess and biosystems engineering 5 (1990), S. 165-167 
    Details
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Required ultracentrifugal time for condensing a solution by ultracentrifuge with an angle rotor or a swing rotor was estimated by considering the calculated distribution of concentration in an ultracentrifugal tube. From the calculated distribution of the concentration a free boundary between solvent and solution was found in the ultracentrifugal tube under the condition that t*〉0.25. A condense constant was newly defined as the ratio of the mean concentration in the ultracentrifugal tube after removing the solvent to the concentration of initial solution. The equation determining the required ultracentrifugal time for condensing a solution up to a given condense constant is derived and solved numerically. The required ultracentrifugal time for an angle rotor was shorter than that for a swing rotor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00369580
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    An index for microcarrier dispersion inside a horizontally rotating bioreactor for animal cell culture (1995)
    Springer
    Bioprocess and biosystems engineering 12 (1995), S. 147-150 
    Details
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract An index of particle dispersion is used to quantify the degree of particle dispersion inside the horizontally rotating bioreactor. Resin particles were suspended inside a horizontally rotating cylinder. The states of particle dispersion were observed and recorded on video, from which data the index of particle dispersion was calculated. The effect of the operating parameters particle concentration and cylinder rotational speed on the state of particle dispersion were described using the index. By comparing the quantitative and qualitative classifications of particle dispersion it was confirmed that the new index does reflect the degree of particle dispersion. From the comparison it can also be concluded that if the index is greater than 0.96, the particle distribution can be considered uniform.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00369592
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of shear stress on activity of cellular enzyme in animal cell (1994)
    Springer
    Bioprocess and biosystems engineering 10 (1994), S. 43-45 
    Details
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The effect of shear stress on the activity of cellular enzyme in an animal cell was discussed by using a flow channel. The activity of lactate dehydrogenase (LDH) in cells exposed to a shear stress of 0.5 Pa for 12 h was about 4-fold greater than in the cells without exposure to shear stress. The relative LDH activity was correlated with the dissipation energy density of the flowing medium. A good correlation was obtained and it was found that the dependency of cellular enzyme activity on the shear stress and the exposure time was related to the transmission of the energy from the flowing medium to attached cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00373534
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Simulated-microcarrier motion and its effect on radial medium transfer inside a horizontally rotating cylindrical bioreactor (HRCB) for animal cell culture (1994)
    Springer
    Bioprocess and biosystems engineering 10 (1994), S. 5-14 
    Details
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The motion and distribution of microcarriers inside a horizontally rotating cylindrical bioreactor were simulated using spherical ion exchange resin particles. Particle motion deviated much from that expected under ideal conditions. The possible mass transfer enhancing effects of particle translation and cluster rotation are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00373529
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    A model for microcarrier motion inside a horizontally rotating bioreactor for animal cell culture (1994)
    Springer
    Bioprocess and biosystems engineering 11 (1994), S. 115-120 
    Details
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The motion of microcarriers inside the horizontally rotating bioreactor was simulated in order to obtain some insight as to how particle motion can affect radial mass transfer. Fluid motion was modeled taking into account momentum transfer induced by particle motion. The force balance on the particle included the viscous drag, inertia, gravitational and buoyant forces. The main characteristics of observed particle motion under conditions of low particle concentration were reproduced by the model. Some implications of particle motion to mass transfer are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00369607
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Development of a new method for testing strength of cells against fluid shear stress (1994)
    Springer
    Bioprocess and biosystems engineering 10 (1994), S. 47-51 
    Details
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A new method for testing the strength of cells against fluid shear stress by using a long capillary column was proposed. The trajectories of cells in the column were simulated by introducing the Brownian motion model. The Brownian motion was performed by the generation of random numbers. The mean exposure time to shear stress and the mean shear stress acting on the surface of cells were discussed by the result of computer simulation. The mean shear stress acting on the surface of cells flowing in the capillary column was estimated as 4/3-fold of the shear stress at the column wall provided that the ratio of the cell radius to the column radius does not exceed 0.08. The effectiveness of this new method for testing the strength of cells against fluid shear stress was shown.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00373535
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Precipitation of protein by ultracentrifuge with angle rotor (1990)
    Springer
    Bioprocess and biosystems engineering 5 (1990), S. 85-88 
    Details
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Precipitation of a protein by ultracentrifuge with an angle rotor was simulated by a model for sedimentation process. Assuming that the concentration of solute in an inclined ultracentrifugal tube is given by averaging the concentration in the imaginary horizontal tube, the governing equation describing the concentration in the rectangular-shaped tube with a uniform field of ultracentrifugal force for an inclined tube in an angle rotor was derived. The exact solution to this governing equation was obtained under the condition that the diffusion is absent or present. The dimensionless concentration which is reduced by the initial concentration can be expressed as the function of a dimensionless ultracentrifugal times ω 2 t in case that the diffusion is absent, and as the function of dimensionless parametersα andt *in case that the diffusion is present. From our first approximated model it is found that the precipitation of a protein by ultracentrifuge with an angle rotor proceeds more rapidly than that with a swing rotor whether diffusion is absent or present.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00589150
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    Paper (German National Licenses)
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  • 8
    Electronic Resource
    Electronic Resource
    Precipitation of protein by ultracentrifuge with angle rotor (1990)
    Springer
    Bioprocess and biosystems engineering 5 (1990), S. 103-105 
    Details
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Precipitation of a protein by ultracentrifuge with an angle rotor was investigated experimentally. Experimental data for Lysozyme and Bovine serum albumin were compared with the calculated distribution of concentration, and an agreement between calculated curves and experimental data during the course of ultracentrifugation was obtained. From the first approximated model presented in our previous paper, it is observed that the precipitation of a protein by ultracentrifuge with an angle rotor proceeds more rapidly than that with a swing rotor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388187
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    Paper (German National Licenses)
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  • 9
    Electronic Resource
    Electronic Resource
    A simple and rapid method for the detection of contaminants in a bioreactor (1990)
    Springer
    Bioprocess and biosystems engineering 5 (1990), S. 115-117 
    Details
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Chromatographic detection of particles with 0.05 to 10.0 μm in diameter by using a capillary tube is reported. Retention times of particles depended on their diameter. Complete peak separation was observed in the chromatogram of a mixture of particles with 0.05 and 10.0 μm in diameter. The application of this technique as detecting method for contaminants in a bioreactor is considered.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388189
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    Paper (German National Licenses)
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  • 10
    Electronic Resource
    Electronic Resource
    Destruxin B, a Specific and Readily Reversible Inhibitor of Vacuolar-Type H^+-Translocating ATPase
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 205 (1994), S. 1358-1365 
    Details
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1006/bbrc.1994.2815
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    Paper (German National Licenses)
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