ISSN:
0749-1581
Keywords:
2D NMR
;
TOCSY
;
ROESY
;
HMQC
;
Carbohydrates
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The primary structure of the de-O-acetylated glucuronoxylomannan isolated from Cryptococcus neoformans serotype D was characterized de novo by NMR spectroscopy. Assignment of the 1H and 13C spectra of the polysaccharide involved a combination of 2D NMR experiments including double-quantum-filtered {1H,1H} COSY, {1H,1H} TOCSY and 1H-detected {1H,13C} heteronuclear multiple quantum coherence spectroscopy. The sequence of the glycosyl residues, including the linkage positions, was deduced by {1H,1H} rotating-frame NOE (ROESY) spectroscopy. The serotype-D glucuronoxylomannan was found to be a branched polymer with a repeating pentasaccharide unit of the following structure: .
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mrc.1260290808
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