ISSN:
1745-4514
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Analyses of amino acids in six samples each of soybean meal, corn and triticale by high-performance liquid chromatography (HPLC) and gas chromatography (GC) were compared. Samples were hydrolyzed in 6N HC1, then phenylthiocarbamyl (for HPLC) and N-heptafluorobutyryl (for GC) derivatives of free amino acids were prepared and analyzed by the appropriate system. Coefficient of variation within each feedstuff for amino acids analyzed by HPLC ranged from 1.8 (leucine) to 23.5% (methionine) in soybean meal, 2.7 (leucine) to 14.7% (methionine) in corn and 1.7 (leucine) to 14.3% (cystine) in triticale. Corresponding figures from GC analysis were 0.7 to 22.4%, 1.4 to 14.3% and 1.6 to 12.1%, respectively. Linear regression of HPLC on GC values gave good fits with R2 ranging from 0.86 for cystine and 0.99 for isoleucine, leucine, lysine, methionine, phenylalanine, threonine, alanine, aspartic acid and glycine. Although some differences between the two systems were evident, feedstuff amino acid values obtained by HPLC and GC analyses compare very well. Results of this study and consideration of such factors as ease of derivatization and length of time for analysis, in our opinion, make HPLC the system of choice.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1745-4514.1988.tb00370.x
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