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  • 1
    Electronic Resource
    Electronic Resource
    Radiation damage effects on the silicon microstrip detector in E789 - A fixed target experiment at fermilab
    Amsterdam : Elsevier
    Nuclear Physics B (Proceedings Supplements) 32 (1993), S. 425-430 
    Details
    ISSN: 0920-5632
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0920-5632(93)90055-B
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Concurrent levels of 11-ketotestosterone in fish surface mucus, muscle tissue and blood (2005)
    Berlin, Germany : Blackwell Verlag GmbH
    Journal of applied ichthyology 21 (2005), S. 0 
    Details
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A rapid, reproducible method is described for extracting and comparing levels of the ether-soluble fish androgen 11-ketotestosterone (11-KT) in blood serum, muscle tissue and surface mucus. Because widely different volumes of extract were recovered after centrifugation from the three sources, it was important to express androgen levels as pg 11-KT/mg of total soluble protein (TSP). For six male and four female sexually-staged freshwater Koi (Cyprinus carpio), the method yielded similar pg 11-KT/mg TSP ratios in blood serum and extracts of muscle tissue and surface mucus, with the strongest correlation between blood serum and surface mucus. While male Koi were distinguishable from females based on the magnitude of 11-KT levels, reproductive stage and gonadosomatic index levels were not correlated with the 11-KT levels of either sex. Similar pg 11-KT/mg TSP ratios were also found for autologous muscle tissue and surface mucus extracts of 37 captured and sexed wild marine fishes representing seven genera. However, high 11-KT levels were not restricted to mature males. Collectively, results suggest that surface mucus collection (followed by 11-KT assay) is a useful alternative to more invasive methods of determining systemic hormone levels in fish. Without knowledge of seasonal variation in levels of this and other sex hormones, however, reliance on 11-KT levels alone may lead to spurious identification of gender, let alone reproductive stage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0426.2005.00650.x
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  • 3
    Electronic Resource
    Electronic Resource
    Rotavirus infection in lambs: Pathogenesis and pathology (1977)
    Springer
    Archives of virology 55 (1977), S. 263-274 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Experimental lamb rotavirus infections were studied by immunofluorescence, histopathology and electron microscopy of tissues from infected gnotobiotic lambs killed at intervals from the incubation period to recovery. The rotavirus was demonstrated by immunofluorescence only in epithelial cells of villi in the small and large intestine, and virus antigen was most abundant during the incubation period. An increased enterocyte turnover rate was suggested by the rapid movement of virus-infected cells to the villus tip, and this increase may be one of the basic pathogenic mechanisms of rotavirus infection. Principal histopathological changes were shortening of villi and sloughing of epithelial cells. These were greatest in the middle and posterior small intestine at the onset of diarrhoea, but regeneration occurred within a few hours. Virus morphology in tissues was similar to that reported in other species, and virus presence correlated well with histopathological change.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01315048
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  • 4
    Electronic Resource
    Electronic Resource
    Detection and transmission of 30 nm virus particles (astroviruses) in faeces of lambs with diarrhoea (1977)
    Springer
    Archives of virology 55 (1977), S. 287-291 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary An outbreak of diarrhoea in lambs was investigated, and electron microscopic examination revealed small round virus-like particles in the faeces from eight of seventeen lambs. A bacteria-free filtrate of faeces from one lamb was given orally to a gnotobiotic lamb, which subsequently excreted virus in faeces. Intestinal contents were collected from this lamb and a filtrate given orally to two further gnotobiotic lambs, which subsequently developed diarrhoea and excreted virus in faeces. The mean diameter of the virus particles was 29.7 nm, and 12 per cent of them showed their surface structure arranged in the form of a six-pointed or five-pointed star. They were similar to those particles previously observed in human infant faeces which were referred to as “astroviruses”. The passage through gnotobiotic lambs with development of diarrhoea showed that these particles were animal viruses which were probably pathogenic for lambs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01315050
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Evidence for serotypic variation among bovine rotaviruses (1984)
    Springer
    Archives of virology 79 (1984), S. 161-171 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Eight field strains of calf rotavirus from the U.K. were compared by neutralisation tests, using convalescent and hyperimmune antisera. Seven of these strains cross-reacted and were considered to be of one serotype, while the 8th was distinguished by a greater than 20-fold two-way difference in neutralisation titre suggesting a second serotype. Three widely-distributed reference strains (U.K., Northern Ireland and Lincoln) cross-reacted with the strains in the dominant serotype, as did 33 of 42 other field calf rotavirus strains. Nine field strains failed to cross-react with either serotype, suggesting the existence of other potential serotypes in the calf population.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01310809
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Serological relationships between rotaviruses from different species as studied by complement fixation and neutralization (1977)
    Springer
    Archives of virology 53 (1977), S. 287-294 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Human, piglet, mouse, foal, lamb, calf and rabbit rotaviruses all infected, but could not readily be subcultured in LLC MK2 cells. Cells infected with mouse and calf rotaviruses reacted by indirect immunofluorescence (FA) with convalescent serum from children, piglets, mice, foals, lambs, calves or rabbits, taken after rotavirus infection. Human, calf, piglet, mouse and foal rotaviruses reacted with human, calf, mouse, foal and lamb convalescent serum by complement fixation (CF). It was not possible to distinguish between different rotaviruses by CF or FA. Neutralization tests, however, detected species-specific rotavirus antigens. Any virus was neutralized by a much higher dilution of homologous species convalescent serum than by any heterologous serum. With the exception of the mouse virus there was very little cross reaction. However, in sera with a very high neutralizing titre for the homologous virus the titre was proportionately raised against heterologous virus. It is, therefore, now possible to type to species an unknown rotavirus by a neutralization test in LLC MK2 cells using convalescent serum from each species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01315627
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Isolation of reovirus type 2 from respiratory tract of sheep (1976)
    Springer
    Archives of virology 52 (1976), S. 143-149 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A virus isolated from a nasal swab taken from a healthy lamb was identified as a reovirus on the basis of morphology and physicochemical characteristics. This virus agglutinated human 0 erythrocytes and was shown by haemagglutination tests to be a type 2 reovirus. Serological evidence indicated infection of other healthy lambs in the same group.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01317873
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  • 8
    Electronic Resource
    Electronic Resource
    Rotavirus infection in lambs: Studies on passive protection (1976)
    Springer
    Archives of virology 52 (1976), S. 201-205 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Gnotobiotic lambs were protected against rotavirus infection by the presence in the gut at the time of infection of colostrum or serum containing antibodies to rotavirus. This protection was observed even when passively-acquired antibody was not present in the serum of the infectod lamb. Infection under these conditions may have conferred immunity to subsequent challenge.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01348017
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  • 9
    Electronic Resource
    Electronic Resource
    Pathogenesis of diarrhoea caused by astrovirus infections in lambs (1979)
    Springer
    Archives of virology 60 (1979), S. 217-226 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Experimental infection of 2-day-old gnotobiotic lambs with lamb astrovirus produced mild diarrhoea after an incubation period of about 48 hours. No other clinical symptoms developed. Infection was studied by immunofluorescent and histological examination of tissues from the lambs. Astroviruses infected only mature villus epithelial cells and subepithelial macrophages in the small intestine, where they produced partial villus atrophy. Infected enterocytes were replaced with cuboidal cells from the crypts, and the lesion gradually healed by 5 days after infection. No serological relationship was detected by immunofluorescence between lamb astrovirus antigen in gut sections and antisera to either calf or human astrovirus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01317493
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  • 10
    Electronic Resource
    Electronic Resource
    Survey of equine rotaviruses shows conservation of one P genotype in background of two G genotypes (1996)
    Springer
    Archives of virology 141 (1996), S. 1601-1612 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary DIG-labelled ssRNA probes were prepared from variable regions of VP4 and VP7 cognate genes, and used in hybridization assays for P and G genotyping of group A cell culture-adapted equine rotaviruses and fecal samples collected from foals with and without diarrhea. The probes confirmed known P and G serotypes of sixteen cell culture-adapted strains. From one-hundred and twenty-one rotavirus-positive samples, 83 reacted when tested for their P and G genotype specific probes. From these, 71 were found to contain G3 P12 genotypes, and 11 G14 P12 genotypes. No sample reacted with H1 or L338 P and G genotype probes. This suggests that the equine rotavirus population is conservative, containing predominantly one P genotype and two G genotypes. One isolate (26/94) whose dsRNA was visualized in an agarose gel did not react with any of the equine probes, and was found to belong to G8 and P1 genotypes. This is the fourth example of a single unique equine isolate (after H1, L338, and R-22). The remaining thirty-eight untypable field isolates had no detectable dsRNA after storage for 1 to 3 years.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01718285
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