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  • 1
    Electronic Resource
    Electronic Resource
    Dynamics of yeast metabolism and regulation (1991)
    Springer
    Bioprocess and biosystems engineering 6 (1991), S. 187-193 
    Details
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The dynamic behavior of yeasts is discussed on the basis of transient experiments, such as pulses and shifts in continuous culture, and of oscillating synchronized cultures. The minimal elements of a structured model are evaluated and extensions by regulatory mechanisms are proposed. Segregation of a population in cell classes reflecting the position of a cell in the cell cycle, its individual age, is shown to be necessary in order to account for the spontaneous synchronization of continuous S. cerevisisae cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00369711
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  • 2
    Electronic Resource
    Electronic Resource
    Pattern recognition: a useful tool in technological processes (1990)
    Springer
    Bioprocess and biosystems engineering 5 (1990), S. 181-187 
    Details
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The introduced method of pattern recognition is a tool for on line control purposes. It is based on the detection of complex states of (bio-)technological processes. The process data are treated in a comprehensive way which allows the formulation and detection of interrelations between different signals. Not only single signals rather than a multiple set defines the state of a process (more) completely. This allows to perform “advanced control” of the “physiological state” of a population and additionally the on line supervision of the technical equipment. Differences in absolute values between corresponding states do not impair the inter-signal associations seriously if the general character of the trajectories remains unchanged. Hence, this approach is very similar to a human view of processes and is suited to serve in expert systems. First results are shown concerning stability of the recognition procedure and a practical approach is introduced performing the automatic detection of defective sensors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00369583
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    On-line capillary gas chromatography with automated liquid sampling, a powerful tool in biotechnology
    Amsterdam : Elsevier
    Analytica Chimica Acta 255 (1991), S. 91-96 
    Details
    ISSN: 0003-2670
    Keywords: Bacillus subtilis metabolites ; Biological samples ; Gas chromatography ; Sample introduction
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0003-2670(91)85091-6
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    ''Intelligent'' analytical subsystems for on-line control and monitoring of bioprocesses
    Amsterdam : Elsevier
    Analytica Chimica Acta 265 (1992), S. 63-69 
    Details
    ISSN: 0003-2670
    Keywords: Bioprocess on-line control ; Flow injection ; Process analysis/on-line analysis
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0003-2670(92)85155-Y
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  • 5
    Electronic Resource
    Electronic Resource
    Dynamics of the respiratory bottleneck of Saccharomyces cerevisiae
    Amsterdam : Elsevier
    Journal of Biotechnology 38 (1994), S. 63-79 
    Details
    ISSN: 0168-1656
    Keywords: Dynamics ; Ethanol ; Mathematical model ; Respiratory capacity, adaption of limited ; Saccharomyces cerevisiae
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0168-1656(94)90148-1
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Advantages of using thermophiles in biotechnological processes: expectations and reality
    Amsterdam : Elsevier
    Trends in Biotechnology 1 (1983), S. 74-80 
    Details
    ISSN: 0167-7799
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0167-7799(83)90056-2
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  • 7
    Electronic Resource
    Electronic Resource
    High-performance bioreactors: a new generation
    Amsterdam : Elsevier
    Analytica Chimica Acta 213 (1988), S. 199-205 
    Details
    ISSN: 0003-2670
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/S0003-2670(00)81353-7
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  • 8
    Electronic Resource
    Electronic Resource
    Application of immobilized cells ofThermoanaerobium brockii for stereoselective reductions of oxo-acid esters (1986)
    Springer
    Applied microbiology and biotechnology 23 (1986), S. 424-429 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Cells ofThermoanaerobium brockii were immobilized by entrapment methods as easy-to-handle biocatalyst for stereoselective reductions of oxo-acid esters. Different matrix materials were tested: agarose, k-carrageenan, alginate, polyacrylamide and polyurethanes. The two latter matrices allowed useful lifetimes of the immobilized biocatalysts of more than 2 months at thermophilic operation temperatures (around 65°C). Permeabilization of cells did not improve the catalytic activity. Immobilization of the cells did not enhance the thermostability. Only after a considerable period of operation could the immobilized biocatalysts be fed with medium lacking the complex substrates yeast extract and tryptone. Compared with freely suspended cells, reaction rates were lower. The immobilized system proved to be a relatively stable easy-to-handle biocatalyst, however, the freely suspended cells were superior with respect to flexibility of application and reaction velocity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02346053
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  • 9
    Electronic Resource
    Electronic Resource
    The growth behaviour of Thermus aquaticus in continuous cultivation (1982)
    Springer
    Applied microbiology and biotechnology 15 (1982), S. 69-74 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The growth of the yellow pigmented non-sporulating caldoactive bacterium Thermus aquaticus was investigated in different culture vessels and using differnt culture techniques. Each combination of these two variables led to very specific characteristic behaviour of the culture. A synthetic medium for a white cell type of T. aquaticus was optimized by means of pulse and medium-shift techniques. The main kinetic parameters of the organism have been determined to be μ=1.62h−1 and Y (glucose)=0.4 g g−1 at T=68 °C and pH=7.3. In complex medium only a mixed population of white and yellow cells could be cultivated. The cell yield was shown to be very low (Y=0.02 g g−1) due to incomplete substrate utilisation, but a very high maximal specific growth rate was determined (μ max=3.5h−1) at 75 °C and pH=7.3. The maintenance coefficient for oxygen uptake was approximately Mo=16 mMol g−1 h−1. A discussion of the problems arising in the cultivation of thermophilic microorganisms with respect to their physiology and stability is given.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00499509
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  • 10
    Electronic Resource
    Electronic Resource
    Growth kinetics of Thermus thermophilus (1982)
    Springer
    Applied microbiology and biotechnology 15 (1982), S. 75-82 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The nonsporulating extreme thermophile Thermus thermophilus was grown in continuous culture at dilution rates up to 2.65 h−1 at 75°C and pH 6.9 on complex medium. Concomitantly very low yield (Y=0.12 g cell dry weight g−1 utilized organic carbon) and incomplete substrate utilization (always less than 45%) were found. In batch cultures T. thermophilus could be grown with μ max =h−1, in shake flasks only with μ max =h−1 with the same low yield and incomplete substrate utilization. Stable steady states at 84δC and 45°C were realized at a dilution rate of 0.3 h−1 whereas at 86°C and 40°C no growth could be detected. Artefacts arising from wall growth (in bioreactors) or improper materials must be ruled out. Inhibition of growth by organic substrates was demonstrated at low concentrations: a decrease in the yield obtained was found when more than 0.7 gl−1 of meat extract were supplied in the medium. The maintenance requirement for oxygen is potentially very high and was determined to be 10 to 15 mmol g−1 h−1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00499510
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