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Detection of antibodies against viral capsid proteins of human herpesvirus 8 in AIDS-associated Kaposi’s sarcoma (1997)

André, Stefanie ; Schatz, Octavian ; Bogner, Johannes R. ; [et al.]

Springer

Journal of molecular medicine 75 (1997), S. 145-152

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ISSN: 1432-1440

Keywords: Key words Antibody ; Capsid antigens ; HHV-8 ; Kaposi’s sarcoma

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract Sequences of a new herpesvirus with homology to gammaherpesvirinae were recently identified in AIDS-associated Kaposi’s sarcoma (KS). Subsequently this novel virus, called KS-associated virus (KSHV) or human herpesvirus (HHV) 8 was detected in classical KS and AIDS-associated body cavity based lymphomas by polymerase chain reaction. In this report major and minor capsid proteins of HHV-8 were molecularly cloned and produced as recombinant proteins in Escherichia coli. Sera from 69 HIV-1 infected patients with KS, 30 HIV-1 infected patients without KS and 106 control individuals were tested by enzyme-linked immunosorbent assay for anti-HHV-8 capsid IgM and IgG antibodies. Sera from four patients were tested over periods ranging from 18 months to 6 years. IgG antibodies directed against HHV-8 capsid antigens were detected in patients with AIDS-associated KS and in some AIDS patients without KS. Seroconversion with IgM and IgG antibodies directed against HHV-8 capsid proteins occurred more than 1 year prior to diagnosis of KS. In a considerable portion of KS patients no IgM or IgG antibodies against HHV-8 capsid proteins were detected. In these patients there was an inverse relationship between antibodies against HHV-8orf26 and the CD4/CD8 ratio, suggesting that the inconsistency of anti-HHV-8orf26 antibodies is due at least partly to an impaired immune response. No reactivity against HHV-8 capsid antigens was detected in the vast majority of sera from HIV-negative control individuals. Our findings indicate that a specific humoral immune response against capsid proteins is raised in HHV-8 infected individuals, and that anti-capsid antibodies can be used to diagnose HHV-8 infection. The correlation between occurrence of anti-HHV-8 antibodies and KS supports the hypothesis of a causative role of HHV-8.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s001090050099

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Evaluation of health services organisations - German experiences with the EFQM excellence approach in healthcare (2001)

Moeller, Johannes ; Sonntag, Anne Katharina

Bradford : Emerald

The @TQM magazine 13 (2001), S. 361-367

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ISSN: 0954-478X

Source: Emerald Fulltext Archive Database 1994-2005

Topics: Economics

Notes: Expands on previous reports by illustrating experiences of German health services organisations made in their assessment against the European Foundation for Quality Management (EFQM) excellence model. Indicates the EFQM method and scoring system. Refers to the experiences German health care pioneers made during their early assessments. Also, success factors and best practices of self-assessments are shared, as well as limitations, barriers, and lessons learned during the implementation phase. The excellence model is a systematic quality management approach to gain competitive advantage. It is non-governmental, non-financier driven, and generic enough to address health care issues. Having its foundation in industry, however, it is not specific enough to cover all areas relevant to health care.