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  • 1
    Electronic Resource
    Electronic Resource
    Molecular Analysis of Proteins in the Plant Plasma Membrane (1994)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Plant Physiology and Plant Molecular Biology 45 (1994), S. 211-234 
    Details
    ISSN: 1040-2519
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.pp.45.060194.001235
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Genetic analysis of tolerance to low-phosphorus stress in maize using restriction fragment length polymorphisms (1991)
    Springer
    Theoretical and applied genetics 82 (1991), S. 561-568 
    Details
    ISSN: 1432-2242
    Keywords: Zea mays ; Restriction fragment length polymorphisms (RFLPs)-Phosphorus (P) stress-quantitative trait loci (QTLs)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An understanding of the genetic nature underlying tolerance to low-phosphorus (low-P) stress could aid in the efficient development of tolerant plant strains. The objective of this study was to identify the number of loci in a maize (Zea mays L.) population segregating for tolerance to low-P stress, their approximate location, and the magnitude of their effect. Seventy-seven restriction fragment length polymorphisms (RFLPs) were identified and scored in a maize F2 population derived from a cross between line NY821 and line H99. The F2 individuals were self-pollinated to produce F3 families. Ninety F3 families were grown in a sand-alumina system, which simulated diffusion-limited, low-P soil conditions. The F3 families were evaluated for vegetative growth in a controlled-environment experiment. To identify quantitative trait loci (QTLs) underlying tolerance to low-P stress, the mean phenotypic performances of the F3 families were contrasted based on genotypic classification at each of 77 RFLP marker loci. Six RFLP marker loci were significantly associated with performance under low-P stress (P〈0.01). One marker locus accounted for 25% of the total phenotypic variation. Additive gene action was predominant for all of the QTLs identified. Significant marker loci were located on four separate chromosomes representing five unlinked genomic regions. Two marker loci were associated with an additive by additive epistatic interaction. A multiple regression model including three marker loci and the significant epistatic interaction accounted for 46% of the total phenotypic variation. Heterozygosity per se was not predictive of phenotypic performance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226791
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Molecular cloning of the plant plasma membrane H+-ATPase (1987)
    Springer
    Plant and soil 99 (1987), S. 185-196 
    Details
    ISSN: 1573-5036
    Keywords: Antibody ; H+-ATPase ; Membrane ; Recombinant DNA ; Transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Mineral transport across the plasma membrane of plant cells is controlled by an electrochemical gradient of protons. This gradient is generated by an ATP-consuming enzyme in the membrane known as a proton pump, or H+-ATPase. The protein has a catalytic subunit of Mr=100,000 and is a prominent band when plasma membrane proteins are analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. We generated specific rabbit polyclonal antibody against the Mr=100,000 H+-ATPase and used the antibody to screen λgtll expression vector libraries of plant DNA. Several phage clones producing immunoreactive protein, and presumably containing DNA sequences for the ATPase structural gene, were isolated and purified from a carrot cDNA library and a Arabidopsis genomic DNA library. These studies represent our first efforts at cloning the structural gene for a plant plasma membrane transport protein. Applicability of the technique to other transport protein genes and the potential for use of recombinant DNA technology in plant mineral transport research are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02370165
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    Paper (German National Licenses)
    Fulltext
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