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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Acta neuropathologica 76 (1988), S. 46-54 
    ISSN: 1432-0533
    Keywords: Polyradiculoneuritis ; Protozoa ; Schwann cell destruction ; Axon degeneration ; Dog
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Four pups in a litter of eight Labrador Retrievers suddenly developed hind limb weakness. In three, paralysis ascended rapidly resulting in quadriplegia, cervical weakness, dysphagia and death. Postmortem examination revealed a severe polyradiculoneuritis in which roots, ganglia, and spinal and cranial nerves were heavily infiltrated by lymphocytes, plasma cells and macrophages and contained abundant protozoan pseudocysts. On sections of the brain and spinal cord protozoa were less frequent and appeared independent of the glial nodules which marked focal areas of necrosis. The organisms innitially were thought to beToxoplasma gondii, but this supposition was not supported by serological, immunocytochemical, or electron microscopic findings. Ultrastructurally the organisms resembled an unidentified sporozoan parasite, which has been reported in the CNS of dogs in Scandinavia. The inflamed spinal roots contained many degenerated and demyelinated axons. Electron microscopic studies indicated that the tachyzoite-like organisms, through their invasive and proliferative activities, brough about many of the degenerative changes in the Schwann cells and axons of the spinal roots and nerves.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Experimental dermatology 6 (1997), S. 0 
    ISSN: 1600-0625
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract Long term cultures of canine keratinocytes have been established but culture conditions currently used require supplementation with fetal bovine serum (FBS). Unfortunately. FBS contains many non-defined components which may interfere with in vitro studies. This study describes the development of defined serum-free culture conditions for neoplastic canine keratinocytes grown submerged and at the air-liquid interface. Two commercially available serum-free media established for human epidermal cells failed to support canine keratinocyte growth. In contrast, a defined serum-free medium developed in our laboratory successfully supported proliferation of neoplastic canine keratinocytes for at least 40 passages. Cells showed a slower growth rate, but reached similar final densities and were morphologically identical to those cultured in FBS. Grown at the air-liquid interface, the cells reached the same degree of differentiation as in vivo stratified squamous epithelium and cultures grown in FBS. These results demonstrate that canine keratinocytes require different serum-free growth conditions than human cells. Neoplastic canine keratinocyte cultures, grown under serum-free culture conditions, provide an ideal in vitro system for comparative studies of keratinocyte biology and pathogenesis of various dermatoses.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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