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  • 1
    Electronic Resource
    Electronic Resource
    High-GC primers are useful in RAPD analysis of fungi (1995)
    Springer
    Current genetics 28 (1995), S. 384-389 
    Details
    ISSN: 1432-0983
    Keywords: Fungi ; High-GC primers ; RAPDs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract For genetic analysis of fungal DNAs, we have modified the RAPD method to use primers with G+C contents of 80–100%. In RAPD analysis of Puccinia graminis f. sp. tritici DNAs, these primers generated twice the number of both amplification products per primer and polymorphisms among isolates as compared to the standard 60–70% G+C primers. With respect to segregation and genetic similarity, RAPD markers generated by the high-GC primers behaved as do RAPD markers produced by the standard primers. These high-GC primers also yielded increased numbers of amplification products in RAPDs on the DNAs of a broad range of other fungi.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00326438
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Physical characteristics of the genome of the phytopathogenic fungus Puccinia graminis (1993)
    Springer
    Current genetics 24 (1993), S. 89-93 
    Details
    ISSN: 1432-0983
    Keywords: Puccinia graminis ; Genome size ; Reassociation kinetics ; Repetitive DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The physical characteristics of the genome of Puccinia graminis f. sp. tritici, the wheat stem rust fungus, were determined by reassociation kinetics. The results indicate that the haploid genome contains 67 Mb and consists of three classes of DNA sequences: (1) 64% unique; (2) 30% repetitive; and (3) 4% foldback. The repetitive sequences have a total complexity of 390 kb and are repeated an average of 52 times. The base composition was 45.3% G+C based on an analysis of the DNA melting temperature. The average amount of DNA per ungerminated urediniospore by diphenylamine assay, corrected for losses during extraction, was 435 fg. This was three times the expected value (147 fg) for dikaryotic spores with nuclei in the G1 phase of the cell cycle, an indication that the spores were in G2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00324670
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Characterization of eight excision plasmids of Pseudomonas syringae pv. phaseolicola (1984)
    Springer
    Molecular genetics and genomics 195 (1984), S. 90-95 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Pseudomonas syringae pv. phaseolicola strain LR719 contains a 150 kilobase pair (kb) plasmid pMC7105, stably integrated into its chromosome. Occasionally, single colony isolates of this strain contain an excision plasmid. Eight unique excision plasmids were selected and characterized by BamHI restriction endonuclease and blot hybridization analyses. These plasmids ranged in size from 35 to 270 kb; the largest contained approximately 130 kb of chromosomal DNA sequences. Restriction maps of pMC7105 were developed to deduce the site of integration and to identify the fragments in which recombination occurred to produce each excision plasmid. The eight excision plasmids were arranged into five classes based on the sites where excision occurs. A 20 kb region of pMC7105, which includes BamHI fragment 9 and portions of adjacent fragments, is present in all excision plasmids and thought to contain the origin of replication. The site of integration on pMC7105 maps within BamHI fragment 8. This fragment shows homology with seven other BamHI fragments of pMC7105 and with five chromosomal fragments identified among the excision plasmids. The data strongly suggest that the integration of pMC7105 may have occurred at a repetitive sequence present on the chromosome and on the plasmid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00332729
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    Paper (German National Licenses)
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