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  • 1
    Electronic Resource
    Electronic Resource
    Expression and transport into mitochondria of bovine cytochrome P-450(SCC) in insect cells using the baculovirus expression system
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 184 (1992), S. 471-477 
    Details
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0006-291X(92)91218-F
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  • 2
    Electronic Resource
    Electronic Resource
    Abnormal glutathione metabolism and increased cytotoxicity caused by H2O2 in human umbilical vein endothelial cells cultured in high glucose medium (1994)
    Springer
    Diabetologia 37 (1994), S. 264-269 
    Details
    ISSN: 1432-0428
    Keywords: Human umbilical vein endothelial cells ; high glucose ; oxygen radicals ; radical scavenger ; glutathione redox cycle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary To determine whether increased oxidative stress in diabetes mellitus is due to an impaired freeradical scavenger function in endothelial cells, GSH-dependent H2O2 degradation in human umbilical vein endothelial cells was studied. The GSH-dependent, NaN3-uninhibitable H2O2-degradation in endothelial cells was reduced by 48% (p 〈0.001) when the cells were exposed to 33 mmol/l d-glucose vs 5.5 mmol/l d-glucose. This impairment was dependent not only on the d-glucose concentration in the medium but also on d-glucose specific metabolism, since neither 27.5 mmol/l l-glucose nor 27.5 mmol/l d-raffinose had any effect on the peroxide degradation activity. Activation of the glutathione redox cycle by H2O2 in cells exposed to high glucose concentrations was attenuated as compared with 5.5 mmol/l d-glucose because of: 1) a 42% decrease (p 〈0.001) in intracellular NADPH content, and 2) a 34% reduction (p 〈0.01) in glutathione release into the media. This results in an accumulation of GSSG in the cells following exposure to H2O2. Both H2O2-evoked 51Cr-release and H2O2-induced endothelial cell damage were significantly (p 〈0.01) greater in the 33 mmol/l d-glucose group than in the 5.5 mmol/l d-glucose group. These results indicate that the abnormal glutathione redox cycle observed in endothelial cells is induced by high glucose concentrations in the medium, resulting in an impairment of reduced GSH-dependent H2O2-degradation. These abnormalities may associate with the increased cellular damage following an exogenous exposure to H2O2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00398053
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  • 3
    Electronic Resource
    Electronic Resource
    Abnormal glutathione metabolism and increased cytotoxicity caused by H2O2 in human umbilical vein endothelial cells cultured in high glucose medium (1994)
    Springer
    Diabetologia 37 (1994), S. 264-269 
    Details
    ISSN: 1432-0428
    Keywords: Key words Human umbilical vein endothelial cells, high glucose, oxygen radicals, radical scavenger, glutathione redox cycle.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary To determine whether increased oxidative stress in diabetes mellitus is due to an impaired free-radical scavenger function in endothelial cells, GSH-dependent H2O2 degradation in human umbilical vein endothelial cells was studied. The GSH-dependent, NaN3-uninhibitable H2O2-degradation in endothelial cells was reduced by 48 % (p 〈0.001) when the cells were exposed to 33 mmol/l D-glucose vs 5.5 mmol/l D-glucose. This impairment was dependent not only on the D-glucose concentration in the medium but also on D-glucose specific metabolism, since neither 27.5 mmol/l L-glucose nor 27.5 mmol/l D-raffinose had any effect on the peroxide degradation activity. Activation of the glutathione redox cycle by H2O2 in cells exposed to high glucose concentrations was attenuated as compared with 5.5 mmol/l D-glucose because of: 1) a 42 % decrease (p 〈0.001) in intracellular NADPH content, and 2) a 34 % reduction (p 〈0.01) in glutathione release into the media. This results in an accumulation of GSSG in the cells following exposure to H2O2. Both H2O2-evoked 51Cr-release and H2O2-induced endothelial cell damage were significantly (p 〈0.01) greater in the 33 mmol/l D-glucose group than in the 5.5 mmol/l D-glucose group. These results indicate that the abnormal glutathione redox cycle observed in endothelial cells is induced by high glucose concentrations in the medium, resulting in an impairment of reduced GSH-dependent H2O2degradation. These abnormalities may associate with the increased cellular damage following an exogenous exposure to H2O2. [Diabetologia (1994) 37: 264–269]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00398053
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  • 4
    Electronic Resource
    Electronic Resource
    Detection of lymph-node metastases in patients with gastric carcinoma: comparison of three MR imaging pulse sequences (2000)
    Springer
    Abdominal imaging 25 (2000), S. 25-29 
    Details
    ISSN: 1432-0509
    Keywords: Key words: Gastric carcinoma—Lymph node—Magnetic resonance—Pulse sequence—Receiver-characteristic-curve analysis.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background: To compare the diagnostic accuracy of magnetic resonance (MR) images obtained with three different pulse sequences for lymph-node metastases in patients with gastric cancer. Methods: T1-weighted spin-echo (SE), breath-hold T2-weighted fast SE, and triphasic gadolinium-enhanced dynamic gradient-recall-echo (GRE) MR images obtained in 16 patients with gastric carcinoma were retrospectively reviewed. Regional lymph nodes were assigned to four different groups, and image review was conducted on a lymph-node group-by-group basis; 64 lymph-node groups were reviewed by two radiologists. Relative sensitivity, specificity, and accuracy were determined based on the findings with definitive surgery and follow-up imaging. Diagnostic accuracy was determined by means of receiver-operating-characteristic (ROC) analysis. Results: Relative sensitivities for lymph-node metastases with T1-weighted SE, breath-hold T2-weighted fast SE, and dynamic GRE images were 61%, 94%, and 59%, respectively. Relative sensitivity with breath-hold T2-weighted fast SE images was significantly greater than that with T1-weighted SE (p 〈 0.05) and dynamic GRE (p 〈 0.05) images. Diagnostic accuracy determined by ROC analysis was marginally higher with breath-hold T2-weighted fast SE (area under ROC curve [Az]= 0.87) than with T1-weighted SE (Az = 0.78, p= 0.08) and dynamic GRE (Az = 0.79, p= 0.12) images. Conclusion: Breath-hold T2-weighted fast SE sequence is useful in the detection of regional lymph-node metastases in patients with gastric carcinoma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/s002619910004
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  • 5
    Electronic Resource
    Electronic Resource
    Hypoosmoregulatory ability of eyed-stage embryos of chum salmon (1995)
    Springer
    Marine biology 122 (1995), S. 165-170 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To evaluate the osmoregulatory ability of eyedstage embryos of laboratory-reared chum salmon, Oncorhynchus keta, we examined changes in osmolality of the perivitelline fluid and blood following transfer to 50 and 100% seawater (SW), together with morphological changes in chloride cells present in the yolk sac membrane. Transfer to SW did not cause any significant change in the whole egg weight. However, the embryos shrank when the eggs were transferred to SW, whereas the perivitelline space increased at the expense of the embryo. Osmolality of the perivitelline fluid increased rapidly to reach environmental levels 3 h after transfer, indicating that the egg shell is permeable to ions and water. Blood osmolality increased after transfer to SW, reached a peak level at 3 h, and then decreased gradually. The chloride cells in the yolk sac membrane became activated following transfer, as shown by increased cell size and frequent appearance of apical openings. These results indicate that the eyed-stage embryos of chum salmon possess hypoosmoregulatory ability and that chloride cells in the yolk sac membrane may be involved in salt extrusion, in place of gill chloride cells, during the late embryonic stage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00349290
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  • 6
    Electronic Resource
    Electronic Resource
    Improvement of oxygen metabolic reserve after extracranial-intracranial bypass surgery in patients with severe haemodynamic insufficiency (1997)
    Springer
    Acta neurochirurgica 139 (1997), S. 52-57 
    Details
    ISSN: 0942-0940
    Keywords: EC-IC bypass surgery ; positron emission tomography ; oxygen metabolic reserve ; cerebrovascular reserve
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effectiveness of extracranial-intracranial arterial bypass (EC-IC bypass) surgery on impaired haemodynamic status was studied in 12 patients with reduced regional cerebral perfusion pressure (rCPP) and elevated regional oxygen extraction fraction (rOEF) in the area distal to the symptomatic arterial lesion. Postoperative positron emission tomography (PET) study demonstrated a statistically significant decrease of rOEF in the operated hemispheres with disappearance of the pre-operative interhemispheric rOEF difference. Regional cerebral blood flow (rCBF) and regional cerebral oxygen metabolism (rCMRO2) were also increased in the operated hemispheres with disappearance of the pre-operative interhemispheric differences. Regional CBF/regional cerebral blood volume (rCBV) ratios of the symptomatic hemispheres were increased after surgery, but were still lower than in the contralateral hemispheres. We conclude that EC-IC bypass surgery improves impaired cerebral oxygen metabolic reserve.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01850868
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  • 7
    Electronic Resource
    Electronic Resource
    MR imaging of multiple cerebral infarctions in angiotropic lymphoma (neoplastic angio-endotheliosis) (1997)
    Springer
    Acta neurochirurgica 139 (1997), S. 478-479 
    Details
    ISSN: 0942-0940
    Keywords: Magnetic resonance imaging ; cerebral infarction ; neoplastic angioendotheliosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01808887
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  • 8
    Electronic Resource
    Electronic Resource
    Haemodynamic ischaemia in paediatric moyamoya disease associated with renovascular hypertension (1997)
    Springer
    Acta neurochirurgica 139 (1997), S. 257-258 
    Details
    ISSN: 0942-0940
    Keywords: Moyamoya disease ; renovascular hypertension ; hemodynamic ischemia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01844761
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  • 9
    Electronic Resource
    Electronic Resource
    Cerebral revasculization in aortitis with occluded bilateral common carotid and left vertebral arteries (1997)
    Springer
    Acta neurochirurgica 139 (1997), S. 375-376 
    Details
    ISSN: 0942-0940
    Keywords: Aortitis ; E-PTFE graft ; cerebral ischaemia ; cerebral revasculization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01808838
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  • 10
    Electronic Resource
    Electronic Resource
    DNA Fragmentation in Central Nervous System Vascular Malformations (2000)
    Springer
    Acta neurochirurgica 142 (2000), S. 987-994 
    Details
    ISSN: 0942-0940
    Keywords: Keywords: Vascular malformations; DNA fragmentation; apoptosis; arteriovenous malformation; cavernous hemangioma.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary ¶ Recent studies have shown that apoptosis plays an important role in vascular remodeling. We examined central nervous system vascular malformations for the presence of DNA fragmentation which is the evidence of apoptosis. We hypothesize that vascular remodeling through apoptosis may be responsible for recurrence or hemorrhage in these lesions. We examined the specimens of central nervous system vascular malformations by in situ end labeling (ISEL) of fragmented DNA. Moreover, we examined the expression of Caspase-3 which is apoptosis-related proteins in these lesions by immunohistochemistry. DNA fragmentation was observed in all 15 arteriovenous malformation (AVM) specimens. ISEL-positive cells were mainly distributed in the endothelium, media and perivascular tissue. In cavernous hemangioma (CH), DNA fragmentation was also observed in all 5 specimens. ISEL-positive cells were distributed in the endothelium, subendothelium and intercavernous matrix. Thirteen out of 15 AVM lesions stained positive for Caspase-3. Caspase-3 immunoreactivity was mainly distributed in the endothelium, media and perivascular tissue. This distribution was similar to that of ISEL positive cells. As for CHs, all 5 lesions stained positive for Caspase-3. Caspase-3 immunoreactivity was distributed in the endothelium, subendothelium and intercavernous matrix. Our findings indicate that apoptotic cell death and vascular remodeling play a role in the development and maintenance of vascular malformations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007010070053
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