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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    International journal of cosmetic science 26 (2004), S. 0 
    ISSN: 1468-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Today, it is said that the formula design of cosmetics from ingredients of plant origin is an indispensable way and trend. From this consideration, cosmetic materials made from animal and synthetic petroleum ingredients are becoming less usable. Instead, cosmetic materials are designed from ingredients of plant origin and many and various botanical ingredients are being developed. Lanolin, which is one of the animal-based ingredients, is said to have ideal functions as a cosmetic oil, and it has been used in many fields such as make-up cosmetics as well as hair and skin care products for a long time. However, unfortunately, lanolin is an animal-based ingredient; therefore, the development of a botanical ingredient to replace lanolin was desired. Polyglyceryl-8 decaerucate/isostearate/ricinoleate, which we have developed, is an ester oil originating from plants and has an equivalent or higher function than lanolin. We have confirmed that our developed ester oil has various excellent characteristics such as a water-holding capability 2.5 times higher than that of lanolin, high air permeability, moisture keeping in dermal layers, protection of hair from changes in external environmental humidity, and excellent gloss and excellent dispersability of pigments. Thus, this newly developed ester oil is expected to be a promising new botanical cosmetic ingredient which can be applied in various fields.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2242
    Keywords: Brassica ; Protoplast fusion ; Asymmetric hybrid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To examine the possibility of producing asymmetric somatic hybrids of Brassica having a complete genome of one species and a part of the other, we fused inactivated B. oleracea protoplasts with X-irradiated B. campestris protoplasts. The plants obtained were studied with regard to their morphology, isozymes and chromosomes. The morphology of the hybrids was similar to B. oleracea in 9 out of 22 hybrids studied and the rest showed the intermediate phenotype of the parents. Analysis of three isozymes, leucine aminopeptidase, acid phosphatase and esterase indicated that ten hybrids lost B. campestris-specific bands in one or more of the three isozymes examined. The chromosome analysis showed that 90% of the hybrids were aneuploids. In addition, abnormal chromosomes were often found in root tip cells. These results suggested that the hybrids obtained were asymmetric in nature and resulted from elimination of B. campestris chromosomes by X-ray irradiation.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2242
    Keywords: Brassica ; Protoplast fusion ; Somatic hybrid ; Selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An efficient procedure for obtaining somatic hybrids between B. oleracea and B. campestris has been developed. Hypocotyl protoplasts of B. oleracea were fused with mesophyll protoplasts from three different varieties of B. campestris by the polyethylene glycoldimethylsulfoxide method. The selection of somatic hybrids utilized the inactivation of B. oleracea protoplasts by iodoacetamide (IOA) and the low regeneration ability of B. campestris. The efficiency of recovery of somatic hybrids depended upon the IOA concentration, and when 15 mM IOA was used, 90% of the regenerated plants were found to be hybrid. The somatic hybrids were examined for i) leaf morphology, ii) leucine aminopeptidase (LAP) isozyme and iii) chromosome number. All the hybrids had intermediate leaf morphology and possessed LAP isozymes of both parental species. The chromosome analysis revealed a considerable variation in chromosome number of somatic hybrids, showing the occurrence of multiple fusion and chromosome loss during the culture. Some of the hybrids flowered and set seeds.
    Type of Medium: Electronic Resource
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