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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of agricultural and food chemistry 18 (1970), S. 199-201 
    ISSN: 1520-5118
    Source: ACS Legacy Archives
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of the American Chemical Society 109 (1987), S. 7214-7215 
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 149 (1987), S. 102-105 
    ISSN: 1432-072X
    Keywords: Reductive dehalogenation ; Chlorobenzoate ; Energy conservation ; Chemotrophy ; Growth yield ; Interspecies hydrogen transfer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The microbially mediated reductive dehalogenation of aromatic compounds is potentially important in removal of chlorinated aromatic compounds from the environment. Thermodynamic data are presented which show that the reductive dechlorination of 3-chlorobenzoate to benzoate is exergonic, which led to the hypothesis that reductive elimination of chlorine from 3-chlorobenzoate yields biologically useful energy. In the present paper this hypothesis is tested. Experimental data were obtained with a defined 3-chlorobenzoate degrading methanogenic consortium. These data showed that (i) the molar growth yield of a defined 3-chlorobenzoate degrading consortium increased from 4.9 g protein per mol benzoate metabolized to 6.8 g protein per mol 3-chlorobenzoate when 3-chlorobenzoate replaced benzoate as energy source, and that (ii) the ATP level in starved consortium cells was twice as high when the cells were fed 3-chlorobenzoate than when fed benzoate. These observations show that the electrochemical potential between the redox partners of the H+/H2 (electron-donating) and 3-chlorobenzoate/benzoate (electron-accepting) couples is a potential source of energy and are consistent with the hypothesis that reductive dechlorination of aromatic compounds is coupled to a novel type of microbial chemotrophy.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of the American Chemical Society 111 (1989), S. 2322-2323 
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Journal of industrial microbiology and biotechnology 18 (1997), S. 68-72 
    ISSN: 1476-5535
    Keywords: Keywords: database; integration; microbes; phylogeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: There are currently 100–200 microbiology-related databases in existence, although it is impossible to find answers to queries that span even a few of these. The Center for Microbial Ecology (CME) at Michigan State University seeks to change this situation by coordinating the creation of an Integrated Microbial Database (IMD), accessible through the World Wide Web (WWW). Such a system will contain up-to-date phylogeny and taxonomy, gene sequences (including genomes), biochemical data, metabolic models, ecological and phenotypic data. Current main obstacles to creation of an IMD are the lack of a single freely available organismal nomenclature with synonyms and the availability of much critical data. An IMD will have major impacts on microbial biology: currently intractable fundamental questions might be answered, experiments could be refocused, and new commercial possibilities created. An IMD should remain freely available and be created under an open development model.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1433-4909
    Keywords: Key words Arctic ; Permafrost ; Subsurface ; Microorganisms ; Psychrotolerant organism ; Low temperature ; Subzero
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Permafrost represents a unique ecosystem that has allowed the prolonged survival of certain bacterial lineages at subzero temperatures. To better understand the permafrost microbial community, it is important to identify isolation protocols that optimize the recovery of genetically diverse bacterial lineages. We have investigated the impact of different low-temperature isolation protocols on recovery of aerobic bacteria from northeast Siberian permafrost of variable geologic origin and frozen for 5000 to 3 million years. Low-nutrient media enhanced the quantitative recovery of bacteria, whereas the isolation of diverse morphotypes was maximized on rich media. Cold enrichments done directly in natural, undisturbed permafrost led not only to recovery of increased numbers of bacteria but also to isolation of genotypes not recovered by means of liquid low-temperature enrichments. On the other hand, direct plating and growth at 4°C also led to recovery of diverse genotypes, some of which were not recovered following enrichment. Strains recovered from different permafrost samples were predominantly oligotrophic and non-spore-forming but were otherwise variable from each other in terms of a number of bacteriological characteristics. Our data suggest that a combination of isolation protocols from different permafrost samples should be used to establish a culture-based survey of the different bacterial lineages in permafrost.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Journal of industrial microbiology and biotechnology 5 (1990), S. 9-15 
    ISSN: 1476-5535
    Keywords: Anaerobic biodegradation ; Chloroaromatic chemicals ; Sulfate reducers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary An obligately anaerobic bacterium known as strain DCB-1 was grown under a variety of conditions to determine the requirements for dehalogenation as well as factors which stimulated or inhibited the process. Dechlorination was obligately anaerobic since introduction of O2 immediately inhibited the reaction. Sulfuroxy anions, which also serve as electron acceptors for DCB-1, inhibited dechlorination but NO3 − and fumarate did not. The optimum growth medium for dechlorination was 0.2% Na pyruvate and 20% rumen fluid in basal salts. Media with either pyruvate or rumen fluid alone did not support dechlorination. DCB-1 also consumed H2 but typical substrate concentrations of H2 (80 kPa) delayed dechlorination. Once the H2 concentration was reduced to 〈20 μM (2.67 kPa), dechlorination resumed. Dehalogenation by DCB-1 was restricted to the meta substituted benzoates as halogens in other positions and chloroaromatic compounds with other functional groups were not dechlorinated.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1573-5036
    Keywords: Forest soils ; Nitrogen cycling ; Nitrogen loss ; N2 ; N2O
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary We examined denitrification and nitrous oxide (N2O) production in intact soil cores removed from a clearcut southern pine site subjected to different harvest, site preparation, and herbicide treatments. Rates of N2O production in structurally intact soil cores incubated with acetylene showed that clearcutting stimulated denitrification but that rates varied by sample date and post-harvest site treatment. The site was harvested in December 1980. In September 1982 denitrification was greater in sheared, piled and disked (SPD) plots than in chopped or reference (uncut) plots; the following May, rates were higher in seven of the eight treatment plots than in the reference plot, and were highest in three of the four herbicide-treated plots. On both sample dates denitrification rates were correlated with nitrification potentials and nitrate pool sizes in the plots, and nitrate added to cores from all treatments significantly stimulated denitrification. Nitrate supply thus appeared to regulate denitrification at this site. Relative to harvest or site preparation losses of nitrogen, denitrification is not a major vector of N loss at this coniferous site; under post-harvest conditions, however, denitrification may be of the same magnitude as leaching losses.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 76 (1984), S. 197-212 
    ISSN: 1573-5036
    Keywords: Aggregate oxygen profile ; Anaerobic radii ; Anaerobiosis ; Anoxia ; Aromatic ring cleavage ; Clostridium ; Dechlorination ; Denitrification ; Denitrification pathway hypothesis ; Fermentation ; Fe3+Mn4+ reduction ; Methanogenic microorganisms ; 13N15N N−N bond N2O ; NO 3 − respiration/dissimilatory reduction ; O2 consumption ; O2 diffusion coefficient ; O2 micro electrode ; Pesticides ; Reduction NO 3 − NO 2 − SO 4 2− CO2H+ ; Soils flooded poorly and well drained ; Sulphate ; Xenobiotics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Anaerobic conditions in soil affect plant productivity as well as organic matter and nutrient dynamics. Anaerobic processes often dominate biological and chemical features of flooded and poorly drained soils but in well-drained soils, anoxia is restricted to small zones and to limited periods. The anaerobic processes listed according to their approximate sequence of occurrence as the redox decreases are: Fe3+ and Mn4+ reduction, denitrification, fermentation, nitrate respiration, dissimilatory nitrate reduction to ammonium, sulfate reduction, carbon dioxide reduction, acetate splitting, and proton reduction. Two of the anaerobic processes, denitrification and fermentation of pollutant chemicals have been studied and recent results are summarized here. We describe the measurement of denitrification using a recirculating atmosphere of acetylene, provide quantitative information on the effect of moisture and oxygen on denitrification, and report on the oxygen concentration within soil aggregates measured by oxygen microelectrodes. The current hypotheses for the pathway leading from nitrate to the N−N bond and N2O are also presented. Recent work in our laboratory has shown a new reaction for the metabolism of some chlorinated organic chemicals. In this reductive dehalogenation, the ring Cl is replaced by a proton. An unusual organism which carried out this reaction has been enriched and isolated on 3-chlorobenzoate. These anaerobic reactions may be of further use in pollutant removal. With these new techniques and knowledge available, it is now possible to gain a better understanding of the biochemistry, physiology, ecology and diversity of the anaerobic organisms and their processes in soil.
    Type of Medium: Electronic Resource
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