ISSN:
1573-5036
Keywords:
Actinorhizae
;
Alnus crispa
;
Alnus viridis spp.crispa
;
Clones
;
Frankia
;
Nodulation
;
In vitro culture
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Summary 600,000 seedlings ofAlnus crispa were inoculated with a 1∶1∶1 mixture of theFrankia strains ACN1 AG , AGN1 exo AG and MGP10i. After 3 successive inoculations and screenings, one individual, AC-4, was selected as non-nodulating (Nod−) with Frankiae. This selected individual AC-4 (Nod−) and two other clones ofA. crispa, AC-2 and AC-5, known for their ability to nodulate (Nod+) and two other clones ofA. crispa, AC-2 and AC-5, known for their ability to nodulate (Nod+) withFrankia werein vitro propagated. The different clones ofA. crispa in culture required different kinds and concentrations of sugar during the in vitro multiplication and rooting stages. Nodulation tests using 7Frankia strains indicated that the clone AC-4 (Nod−) was non-nodulating with 6 of the 7Frankia strains tested. One strain,Frankia ANNI, isolated from one unique nodule produced on the mother-plant AC-4, induced 38% of the AC-4 plantlets to nodulate but with a number of nodules 10 to 20 times less than the clones AC-2 (Nod+) and AC-5 (Nod+). Morphological observations of the roots of AC-4 (Nod−) indicated that this clone had few and abnormally short root hairs.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02277848
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