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  • 1
    ISSN: 1432-0428
    Keywords: Nodular glomerulosclerosis ; Kimmelstiel-Wilson nephropathy ; long-acting heterologous insulin ; histoimmunology ; electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Des lésions rénales semblables à la néphropathie de Kimmelstiel-Wilson ont été trouvées chez des cobayes immunisés avec de l'insuline hétérologue à action prolongée. — Les animaux ont été traités pendant des périodes de 3 à 5 mois par des injections mensuelles souscutan ées d'insuline bovine hautement purifiée, mélangée à de la lanoline et à de l'huile de paraffine. — Les coupes de rein ont été examinées à l'aide du microscope à lumière, après colorations standard et histochimiques, ainsi qu'à l'aide de techniques histoimmunologiques et au microscope électronique. — On a trouvé les lésions glomérulaires suivantes: 1. Nodules hyalins PAS-positifs (55.5%); 2. épaississement de la membrane basale (100%); 3. dilatation anévrismale des capillaires (88.8%); 4. glomérulosclérose diffuse (61.1%); 5. augmentation du nombre des cellules du mésangium (38.8%); 6. capsules fibrinoïdes (44.4%); 7. adhérences capsulaires (33.3%). — Les résultats histochimiques ont montré qu'il y a des caractéristiques diff érentielles entre les capsules fibrinoïdes (lésions exsudatives) et les altérations nodulaires hyalines: les premières sont digérées rapidement par la trypsine tandis que les nodules résistent au traitement de 6 h par la trypsine. — La coloration histoimmunologique des coupes de rein lyophilisé, avec du sérum anti-insuline de boeuf marqué avec del'isothiocyanate de fluorescéine montra une fluorescence prononcée des capsules fibrinoïdes et des nodules hyalins des glomérules, ainsi qu'une fluorescence moins intense de la membrane basale et du stroma intercapillaire. — La microscopie électronique des glomérules a montré que la membrane basale est toujours épaissie de façon irrégulière, présentant une structure en taches, et qu'il y a des aires nodulaires et des bandes de matière semblable à la membrane basale. En outre on a noté des dépôts osmophiles denses dans le mésangium. — On conclut que l'insuline agissant comme un antigène provoque, par l'intermédiaire de mécanismes immunologiques, une néphropathie nodulaire semblable à celle de Kimmelstiel-Wilson, chez les animaux d'expérience ayant un taux normal ou bas de sucre et de lipides.
    Abstract: Zusammenfassung Nach Immunisierung durch heterologes Insulin mit protrahierter Wirkung ließen sich bei Meerschweinchen ähnliche Nierenveränderungen wie bei der Kimmelstiel-Wilson'schen Nephropathie nachweisen. — Die Tiere erhielten monatliche subcutane Injektionen von hochgereinigtem Rinderinsulin in einer Mischung von Lanolin und Paraffinöl über einen Zeitraum von 3–5 Monaten. — Die Untersuchung der Nierenschnitte erfolgte lichtmikroskopisch mit Hilfe von Standardund histochemischen Färbungen sowie durch histoimmunologische Verfahren und die Elektronenmikrosko pie. Dabei ließen sich an den Glomeruli folgende pathologische Befunde erheben: — 1. PAS-positive hyaline Knötchen (55.5%); 2. eine Verdickung der Basalmembran (100%); 3. aneurysmatische Kapillarerweiterungen (88.8%); 4. eine diffuse Glomerulosklerose (61.1%); 5. eine Vermehrung der mesangialen Zellen (38.8%); 6. Fibrinausfällungen (44.4%); 7. Kapseladhäsionen (33.3%). — Die histochemischen Untersuchungen zeigten Unterschiede zwischen den Fibrinausfällungen (exsudative Veränderungen) und den hyalinen Knötchen: erstere wurden durch Trypsin schnell zerstört, während die hyalinen Knötchen einer Trypsinbehandlung über 6 Std. standhielten. Histo-immunologische Anfärbung lyophlisierter Nierenschnitte durch Anti-Rinderinsulin-Serum, das mit Fluoreszin-Isothiozyanat markiert worden war, ließen eine ausgeprägte Fluoreszenz der Fibrinausfällungen, sowie der hyalinen Knötchen in den Glomeruli und eine schwächere Fluoreszenz der Basalmembran und des interkapillären Grundgewebes erkennen. Durch die Elektronenmikroskopie konnte nachgewiesen werden, daß die Basalmembran immer unregelmäßig verdickt war und stellenweise eine fibrilläre Struktur aufwies. Weiter fanden sich noduläre Bezirke und Streifen von membran-„ähnlichem “ Material. Im Mesangium ließen sich ferner dichte osmophile Ablagerungen nachweisen. Es wird gefolgert, daß Insulin als Antigen über immunologische Mechanismen bei Versuchstieren mit normalen oder sogar erniedrigten Glucoseund Lipid-Serumspiegeln eine noduläre Eämmelstiel-Wilson „ähnliche“ Nephropathie auslösen kann.
    Notes: Summary Renal lesions similar to Kimmelstiel-Wilson nephropathy have been found in guinea pigs immunized with long-acting heterologous insulin. — The animals were treated for periods ranging from 3 to 5 months, with monthly subcutaneous injections of highly purified bovine insulin in a mixture of lanolin and paraffin oil. — Kidney sections have been examined by means of light microscopy after standard and histochemical stainings, histoimmunological techniques and electron microscopy. — The following glomerular lesions have been detected : 1. PAS-positive hyaline nodules (55.5%); 2. Thickening of the basal membrane (100%); 3. aneurysmatic dilatation of the capillaries (88.8%); 4. diffuse glomerulosclerosis (61.1%); 5. increased number of mesangial cells (38.8%); 6. fibrinoid caps (44.4%); 7. capsular adhesions (33.3%). — Histo-chemical findings showed that there are differential characteristics between fibrinoid caps (exudative lesions) and hyaline nodular alterations: the former were rapidly digested by trypsin, whereas the nodules resisted the tryptic treatment for 6 h. — Histoimmunological staining of the lyophilized kidney sections with fluorescein-isothiocyanate-labelled anti-bovine insulin serum showed marked fluorescence of both the fibrinoid caps and hyaline nodules of the glomeruli, and also a less intense fluorescence of the basement membrane and of the intercapillary stroma. — Electron microscopy of the glomeruli demonstrated that the basal membrane was always irregularly thickened showing a patchy fibrillary structure, and that there were nodular areas and bands of “basal-membrane-like” material. Besides, dense osmiophilic deposits were noted in the mesangium. — It is concluded that insulin acting as an antigen brings about a nodular Kimmelstiel-Wilson-like nephropathy in experimental animals with normal or low blood sugar and lipids, through immunological mechanisms.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1238
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Conclusions The results of our study suggest that the presence of fat droplets in more than 5% of alveolar macrophages may be present in patients with long bone fractures but is also present in patients without this type of traumatic lesion. We conclude that NB-pBAL, in contrast to fiberoptic bronchoscopic lavage (pBAL), is a specific but not sufficiently sensitive method for the diagnosis of fat embolism syndrome in critical care patients.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1043-4666
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 0009-8981
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 114 (1986), S. 0 
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Alimentary pharmacology & therapeutics 21 (2005), S. 0 
    ISSN: 1365-2036
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background : Dialysis patients remain a high-risk group for hepatitis C virus infection. The current diagnosis of hepatitis C virus in dialysis patients includes serological measurement of anti-hepatitis C virus antibody; however, nucleic acid amplification technology for assessing hepatitis C virus viraemia is commonly used in other populations. An enzyme-linked immunosorbent assay test for detecting antibody to hepatitis C nucleocapsid core antigen (hepatitis C virus core antigen) in human serum has been recently developed (hepatitis C virus Core Antigen enzyme-linked immunosorbent assay test). It is conceived for screening of donor blood products to significantly reduce the ‘serologic window’ occurring before seroconversion during acute hepatitis C virus.Aim and methods : A cohort (n = 72) of patients on maintenance haemodialysis in a single unit in the years 2000–2003 was included. Study patients were tested monthly by hepatitis C virus Core Antigen enzyme-linked immunosorbent assay in a prospective, clinical trial. Routine results obtained by hepatitis C virus Core Antigen enzyme-linked immunosorbent assay test were confirmed by assessing hepatitis C virus viraemia by branched-chain DNA (bDNA) signal amplification assay.Results : De novo hepatitis C virus infection was identified in three patients during the study period; the hepatitis C virus incidence was 1.38% (95% confidence intervals, 1.31–4.09) per year. In each patient, hepatitis C virus core antigen testing allowed the serological identification of acute hepatitis C virus before anti-hepatitis C virus seroconversion. Hepatitis C virus RNA testing confirmed the results obtained by hepatitis C virus Core Antigen enzyme-linked immunosorbent assay in all cases. The time from initial hepatitis C virus detection by hepatitis C virus Core Antigen Assay and anti-hepatitis C virus seroconversion was not greater than four weeks. Two (67%) of three patients with de novo hepatitis C virus acquisition were HBsAg negative; both these patients underwent an initial phase of hepatitis C virus viraemia that was associated with an increase in alanine aminotransferase activity and preceded the seroconversion to anti-hepatitis C virus antibody. Nosocomial transmission of hepatitis C virus between haemodialysis patients was implicated in at least two (67%) of these three patients.Conclusions : Serological testing for hepatitis C virus core antigen can identify acute hepatitis C virus infection before anti-hepatitis C virus seroconversion. The time from initial hepatitis C virus detection by hepatitis C virus core antigen assay and anti-hepatitis C virus seroconversion was not 〉4 weeks. De novo acquisition of hepatitis C virus in haemodialysis was associated with a rise in alanine aminotransferase levels. Hepatitis C virus core antigen enzyme-linked immunosorbent assay test results can be obtained in routine laboratories without the need of special equipment or training. Hepatitis C virus core antigen testing among anti-hepatitis C virus negative patients on maintenance dialysis is suggested in order to early assess de novo hepatitis C virus within dialysis units.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 32 (1976), S. 1600-1602 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Immunological studies, performed with human sera and rabbit antisera upon some strains ofStaph. epidermidis carried in the circulating blood of normal and thrombocytopenic subjects, indicate that the reactivity is very low and almost completely related to antigenic properties common toStaph. aureus.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-0584
    Keywords: Histiocytes ; Immunophenotype ; Chromosomes ; c-fms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We describe a case of “sinus histiocytosis with massive lymphadenopathy” (SHML) studied by immunohistochemical, cytogenetic and molecular analysis. The immunophenotyping showed that the lymph node histiocytes were strongly positive for the S-100 protein and MoAb LeuM3, OKM5, KP1 and DRC-1; a portion of these cells was also positive for OKT6 and Leu3A, suggesting a possible relationship with the veiled cells, which represent an intermediate step in the pathway from the Langerhans cell to the interdigitating reticulum cell. Cytogenetic analysis showed a normal prevalent clone and a small hypodiploid clone and the molecular study showed no detectable involvement of the c-fms proto-oncogene, which is related to monocyte/macrophages. Unfortunately all these data do not seem sufficient to define the benign or neoplastic nature of the disease. Further investigations, immunophenotypical, cytogenetic and molecular, are needed to elucidate the pathogenesis of the disease, especially for more aggressive cases or for cases with unfavorable evolution.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1432-0584
    Keywords: Essential thrombocythemia ; Interferon alpha ; Long-term treatment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We treated 35 patients affected with essential thrombocythemia (ET) with interferon (IFN) alpha-2b. Our treatment scheme consisted of (a) a 6-month induction phase and (b) a continuous maintenance phase. During the induction phase, our results showed that using 21 million units (mu) of IFN weekly platelet counts fell below 600×109/l in about 90% of patients. These data demonstrate that well-tolerated doses of IFN can rapidly correct excessive thrombocytosis. During the continuous maintenance phase, 61% of patients required 3 mu three times a week, 15% once a week, and 24% daily. Thus the minimal IFN doses able to maintain platelet count below 600×109/l varied between 3 and 21 mu per week. During long-term treatment, subjective side effects were tolerable, especially using 3 mu three times a week. We conclude that IFN alpha-2b is an effective drug in the long-term treatment of ET.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-0584
    Keywords: Essential thrombocythemia ; Interferon-α ; Sustained remission
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In essential thrombocythemia patients α-IFN rapidly reduces platelet count, and it is also able to maintain a low count during long-term treatment. In order to verify if long-term IFN treatment can produce sustained remission in selected patients, we decided to suspend IFN treatment in two subsets of 21 patients on long-term α-IFN treatment: (a) all six patients who had shown a platelet count below 450×109/l for at least 2 months with 3 MU once a week; (b) three patients who had shown the same platelet count for at least 2 months with 3 MU three times a week. After withdrawal of α-IFN treatment, a rapid increase in the platelet count was observed in all three patients requiring 3 MU three times a week. Three of the six patients treated with 3 MU once a week are still free of symptoms and have been in complete hematological remission (platelet count below 450×109/l) for 9+, 13+, and 14+ months, respectively. As far as the three remaining cases are concerned, one was not assessable because of loss to follow-up, while the other two relapsed after 1 and 2 months. We believe that the three cases of sustained remission might be the result of a long-term tumor load reduction produced by the α-IFN treatment. Finally, the factor best able to predict sustained, unmaintained remission seems to be the clinical response to a low dose of α-IFN during the maintenance phase, rather than disease features prior to treatment.
    Type of Medium: Electronic Resource
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