ISSN:
0006-3592
Keywords:
transient response method
;
rate constants
;
immobilized enzyme
;
glucose oxidation
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
The transient response method was utilized to evaluate the rate constants of reaction over immobilized enzyme. Glucose oxidation catalyzed by the immobilized glucose oxidase in a fixed-bed reactor was selected as an example. A theoretical model including the effects of axial dispersion, film diffusion, and intraparticle diffusion was established for the reactor. The individual rate constant of each elementary step of this enzymatic reaction was determined through direct fitting of the experimental response data to the model.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bit.260371005
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