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Derivation of mouse intestinal crypts from single progenitor cells (1985)

Ponder, B. A. J. ; Schmidt, G. H. ; Wilkinson, M. M. ; [et al.]

[s.l.] : Nature Publishing Group

Nature 313 (1985), S. 689-691

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ISSN: 1476-4687

Source: Nature Archives 1869 - 2009

Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics

Notes: [Auszug] Mouse embryo aggregation chimaeras were prepared at the MRC Laboratories, Carshalton, as described previously4. The mosaic cell populations in the intestinal epithelium of the chimaeras were demonstrated using H-2 antigens4,5 and a carbohydrate polymorphism recognized by Dolichos biflorus ...

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1038/313689a0

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Immunohistochemistry in the analysis of mouse aggregation chimaeras (1986)

Ponder, B. A. J. ; Schmidt, G. H. ; Wilkinson, M. M.

Springer

Journal of molecular histology 18 (1986), S. 217-227

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ISSN: 1573-6865

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Summary We have used cellular mosaicism in chimaeric mice to study the clonal organization of normal tissues. The mosaicism has been demonstrated in sections and in whole mounts of intestinal epithelium, aortic endothelium and retinal pigment epithelium using H2 antigens and a carbohydrate polymorphism recognized byDolichos biflorus lectin as strain-specific markers. The results show that the epithelium of each adult intestinal crypt is derived from a single progenitor cell. Because crypts of differing genotype may contribute cells to the same villus, the pathways of cell migration up the villi can be demonstrated. The ability to stain mosaic patches in two dimensions in large intact sheets of epithelium has permitted a more satisfactory analysis in terms of clonal development than was previously possible with data from tissue sections. We have adapted statistical procedures from plant ecology to examine the scale of clustering of patches of like genotype, and thence to recognize ‘descendent’ clones, i.e. groups of cells which are not contiguous, but are related by descent from a common ancestor in embryogenesis.