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  • 1
    ISSN: 1432-041X
    Keywords: Key words trachealess ; Silk gland ; Trachea ; Bombyx mori
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We describe embryonic development of the Bombyx silk gland. To extend the analysis further we isolated a Bombyx counterpart gene ofthe Drosophila trachealess (trh) gene. Bombyx trh encodes a protein of 849 amino acids. When compared with the amino acid sequence of Drosophila trh, the identity of Bombyx bHLH, PAS-A and PAS-B domains is 100%, 97%, and 80%, respectively. Northern blot analysis revealed the presence of a single Bombyx trh transcript of 5.4 kb. We analyzed the expression pattern of the Bombyx trh transcript during embryogenesis by in situ hybridization. Bombyx trh mRNA was first detected in the tracheal primordial cells at around embryonic stage 18. Thereafter levels of Bombyx trh mRNA increased, and the high expression level was maintained until hatching. At embryonic stage 19 the transcript was also detected in the posterior basal region of the labial segment from where the silk gland invaginates. By the blastokinesis stage (around stage 23), the silk gland was lengthened, and, interestingly, the Bombyx trh transcript was restricted to the anterior silk gland. These results suggest that Bombyx trh plays a role in the formation of the trachea and the anterior silk glands.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Development genes and evolution 206 (1997), S. 494-502 
    ISSN: 1432-041X
    Keywords: Key words POU domain ; SGF-3 ; Cf1a ; Silk gland ; Salivary gland
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The expression pattern of the POU-M1/SGF-3 gene during Bombyx embryogenesis has been analysed using in situ hybridization and immunohistochemistry. At the early embryo retraction stage, both the transcripts and protein were first detected in precursor cells of the prothoracic glands in the labial segment, in the oenocytes in the A1–A8 segments and in invaginated regions in the mandibular and maxillary segments. The invaginated regions in the mandibular segment develop into the abductor plates in the lateral anterior region and the adductor plates in the posterior region. From the latter plates, the salivary glands elongate. The invaginated regions in the maxillary segment develop into the corpora allata in the anterior region and the subbuccal glands in the posterior region, which unite with tissues of the anterior region of the mandibular segment at later stages. After the embryo retraction stage, the transcripts and protein products also become detectable in the silk gland invagination points and, after the blastokinesis stage, the products are restricted to the entire anterior silk glands and to the anterior and middle parts of the middle silk glands. Expression can also be detected in a part of the hindgut, in the tracheal system and in some cells of the central nervous system. These results indicate that POU-M1/SGF-3 might play roles in the development of the silk glands, nervous system, tracheal system and other organs like the prothoracic glands and oenocytes.
    Type of Medium: Electronic Resource
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