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  • 1
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 330-332 (Feb. 2007), p. 1197-1200 
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: To evaluate the effectiveness of the cell-material in situ on joint resurfacing, a wovenfabric polyglycolic acid (PGA) treated with fresh chondrocytes was used for repairing cartilagedefects. Full-thickness defects were created in the weight-bearing surfaces of the femoralintercondylar fossa in a rabbit model. The defect was filled with and without PGA under surgicalcondition. Before implantation, chondrocytes were co-cultured with PGA for one day. The animalswere sacrificed at eight weeks after implantation and evaluated grossly and histological score.Morphological examination showed that for PGA/chondrocytes group, the repaired tissue appearedsimilar in color and texture to the surrounding articular surface. While for the untreated control, nocartilage-like tissue was observed at all defects, but connective fibrous tissue. Histological analysisrevealed neochondrogenesis and clusters of cartilage matrix with specific safranin-O staining for thePGA/cell group. The Gross and histological evaluation indicated a significantly higher score forPGA/cell group than for PGA and control group. These results suggest that the woven fabric PGAmay facilitate the formation of cartilage tissues by providing a biodegradable and good-handlevehicle for the delivery to and retention of organized cell matrix constructs in vivo site. It mighttherefore enhance neochondrogenesis because of the superior biodegradable and biocompatible ofPGA scaffold sheet, while the more suitable biological environment might sustain cell growth andin situ cell function, suggesting a promising candidate for functional tissue engineering of clinicalenvironment
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 284-286 (Apr. 2005), p. 941-944 
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Stimulation of bone healing through local application of growth factors from implantsmay improve the clinical outcome in fracture treatments. However, the growth factors in reconstructive application require supraphysiologic dosing and considerable expense while hampering their clinical application. Genetic modification of mesenchymal stem cells (MSCs) to both produce and respond to osteogenic factors may have potential for use in enhancing bone healing. In this study, MSCs were genetically modified by a recombinant adenoviral containing the gene for human bone morphogenetic protein 2 (hBMP-2). The gene-transduced cells wereincorporated with a porous beta-tricalcium phosphate (TCP) as a novel complex. We investigated osteogeneic potential of gene-transduced MSCs/ceramic and the ability of the complex on facilitating bone formation in a radius segmental defect of rabbits. In vitro results showed that there were apparent hBMP2 gene expression and protein synthesis in MSCs with hBMP2 stably transfection, whereas negative expression of hBMP2 in controls. Histological studies demonstrated that gene-transfected MSCs/ceramic composite appeared an ability of heterotopic osteogenesis. In the segmental bone defects, endochondrial ossification at fracture sites was found in both transfected and untransfected MSCs-ceramic composites. While the composite with hBMP2 transfection showed the earliest and the most effective healing of the segmental bone defects both radiographically and morphologically. Our results show that genetically modified MSCs/ceramicshad enhanced osteogeneic capacity relative to unmodified MSCs or only ceramic implants. This study suggests that use of cell-and gene-activated bioceramics may offer promise for molecular design of implants to induce osteogenesis and enhance bone regeneration
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 361-363 (Nov. 2007), p. 837-840 
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: In the present investigation, four titanium (Ti) surfaces of dental implants were comparedthrough in vitro systems. The surface roughness of Ti was measured by TR240 mobile surfaceroughmeter. The Ti implants were seeded with human periodontal ligament cells (hPLDCs) andmaintained for a period of 0-7 days. The adhesion, proliferation, and differentiation of hPLDCswere observed by using Cell morphology, cell counting and Osteocalcin (OC) immunofluorescentstaining. Results suggest that surface roughness of titanium favors hPDLCs behavior and improvescell adhesion, proliferation, and differentiation
    Type of Medium: Electronic Resource
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