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  • Electronic Resource  (6)
  • 1995-1999  (4)
  • 1990-1994  (1)
  • 1980-1984  (1)
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  • Electronic Resource  (6)
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  • 1
    ISSN: 1432-0428
    Keywords: Keywords Leptin ; leptin receptor ; Ob-R ; obesity ; sequence variant.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Leptin is an adipocyte-derived blood-borne satiety factor that acts on its cognate leptin receptor (Ob-R) in the hypothalamus, thereby regulating food intake and energy expenditure. To explore whether mutations in the Ob-R gene cause obesity in humans, we have searched for mutations in the gene for Ob-Rb, a biologically active receptor isoform, in obese Japanese subjects. We have also examined associations between such mutants and obesity in the Japanese. Genomic DNAs were used as templates in polymerase chain reaction (PCR) with primers selected to amplify exons 2 to 20 of the human Ob-Rb gene. Direct sequence analysis of the PCR products revealed 7 nucleotide sequence variants (Lys109Arg, Gln223Arg, Ser343Ser, Ser492Thr, Lys656Asn, Ala976Asp, and Pro1019Pro) in the Ob-Rb coding region from 17 obese Japanese subjects with a family history of obesity (BMI 39.3 ± 8.4 kg/m2). No missense and nonsense mutations were found such as those in Zucker fatty (fa/fa) rats and Koletsky (fa k /fa k) rats. Nucleotide substitutions occurred at relatively high frequencies at codons 109, 223, 976, and 1019 (79, 91, 100, and 85 %, respectively). Allele frequency of each variant determined by PCR-RFLP and PCR-single strand conformation polymorphism analyses showed no significant differences between 47 obese (BMI 35.1 ± 6.5 kg/m2) and 68 non-obese (BMI 21.6 ± 2.2 kg/m2) subjects. The present study represents the first report of sequence variants of the Ob-Rb gene in the Japanese and provides evidence against either obesity-causing mutations or association of sequence variants with obesity in obese Japanese subjects. [Diabetologia (1997) 40: 1204–1210]
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Clinical and experimental pharmacology and physiology 22 (1995), S. 0 
    ISSN: 1440-1681
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: 1. To elucidate the functional implication of very low density lipoprotein (VLDL) receptor, we studied the gene expression of VLDL receptor in rats. The VLDL receptor mRNA was highly expressed in the cardiac ventricle and skeletal muscle. Intermediate amounts of VLDL receptor mRNA were detected in adipose tissue, adrenal gland, brain and lung. Thus the tissue distribution of VLDL receptor mRNA in rats was similar to that reported previously in rabbits.2. We studied the gene expression of the VLDL receptor in the heart of stroke-prone spontaneously hypertensive rats (SHRSP), an animal model for hypertension-induced cardiac hypertrophy. RNase protection assay showed that the level of ventricular VLDL receptor mRNA was already decreased to one half when hypertension was not fully developed, and further diminished to one fifth when cardiac hypertrophy was established.3. It is reported that energy utilization in SHRSP hypertrophied myocardium is impaired. Our results suggest that inactive fatty acid metabolism in the ventricle of SHRSP is related to the lowered expression of the VLDL receptor which is postulated as a gate for triglyceride-rich lipoprotein particle.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Microwave digestion was applied as pretreatment of environmental samples for the determination of lead isotope ratios by ICP-MS. Microwave digestion decomposed the samples easily and rapidly without lead contamination. The lead isotope ratios could be measured directly by ICP-MS without tedious lead purification, because the influence of the matrix elements on measurements of lead isotope ratios is small enough to be ignored.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Journal of radioanalytical and nuclear chemistry 230 (1998), S. 97-104 
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The concentration and size distribution of210Po in particulate matters in the atmosphere were measured around the active volcano, Mt. Sakurajima in Kagoshima prefecture, Japan. The samples were collected eight times at four sampling points for the period from June 1994 to January 1996. The highest concentration of210Po was 2940 μBq/m3 at Akamizu located 2 km away from the crater of Mt. Sakurajima. The210Po concentrations decreased with the increase of distance from Mt. Sakurajima. The size distribution curves of210Po in the particulate matters showed that210Po is usually condensed to fine particles smaller than 2 μm in diameter. In addition, it was suggested that the210Po concentration in particulate matters collected at Akamizu was affected by the wind direction over Mt. Sakurajima.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Journal of radioanalytical and nuclear chemistry 140 (1990), S. 57-73 
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract A radioanalytical procedure has been developed for the determination of99Tc in environmental samples. The procedure consists of precipitation, solvent extraction, ion exchange, electrodeposition and radiation measurement. Rhenium was used as a non-isotopic carrier of99Tc. On the basis of 3σ counting error, the detection limits were 4.9·10−5 Bq/l, 7.4·10−3 Bq/kg wet and 7.4·10−2 Bq/kg dry for water, biota and soil samples, respectively. Sea water, seaweeds (brown algae) and soils were collected to evaluate the present levels of99Tc in Japan. The level of99Tc in sea water was ca. 1·10−4 Bq/l around Japan. Among the seaweeds (brown algae), Ishige okamurai showed the highest concentration of 5.8·10−2 Bq/kg wet tissue and the highest concentration factor of 583. The level of99Tc in the organic rich surface soil was ca. 1 Bq/kg dry soil in Fukuoka.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Journal of radioanalytical and nuclear chemistry 81 (1984), S. 161-165 
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract A portable apparatus was developed for the rapid collection, separation and counting of krypton-85 in the environmental air samples. The apparatus consists of a molecular sieve trap for the elimination of H2O and CO2, a charcoal trap for the collection of krypton, a chromatographic column for the isolation of krypton in the air sample and a vial for the dissolution of krypton in the scintillation solution. With this apparatus, krypton can be collected from half a cubic meter of air with 94% recovery in about 3 hrs. The measurements of krypton-85 have been done several times in Fukuoka area between the periods Jan. 30, 1981 and Dec. 9, 1981. And then, the krypton-85 concentration was found to be approximately 21 pCi/m3 air. The results were compared with those obtained by foregin investigators.
    Type of Medium: Electronic Resource
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