ISSN:
1432-2013
Schlagwort(e):
Pancreas
;
Fluid secretion
;
Amylase secretion
;
Ca2+ dependency
;
Caerulein
;
Secretin
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Medizin
Notizen:
Summary In the perfused rat pancreas fluid and amylase secretion was measured in the absence of stimulation and during stimulation with caerulein or secretin. Taking away perfusion fluid Ca2+ only slightly reduced spontaneous fluid secretion, but caused a marked reduction in the amylase output. In experiments where the CO2/HCO 3 − -buffer had been replaced by a tris buffer removal of perfusion fluid Ca2+ abolished spontaneous fluid and amylase secretion. Taking away perfusion fluid Ca2+ during continuous stimulation with caerulein caused an immediate rapid reduction in fluid and amylase secretion which was reversible upon readmission of Ca2+. Removal of perfusion fluid Ca2+ and addition of EGTA during stimulation with secretin had no effect on fluid secretion for the first half an hour after start of Ca2+ deprivation. Thereafter a gradual reduction in fluid flow occurred which was non-reversible upon Ca2+ readmission. During stimulation with monobutyryl cyclic AMP Ca2+-deprivation failed to reduce fluid secretion within a period of half an hour. Augmenting the perfusion fluid [Ca2+] to 20 mM during stimulation with caerulein caused a sharp reduction in fluid secretion and a small decrease in amylase output. These effects were partially reversible if the period of exposure to the high Ca2+ solution was less than 20 min. It is concluded that extracellular Ca2+ is important for caerulein-evoked but not for secretin-evoked fluid secretion.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00581692
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