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  • 1
    Electronic Resource
    Electronic Resource
    The transport of freshwater fish (1975)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 7 (1975), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A method is described for the transport of fish resulting in very few or no mortalities during or after this procedure. Commercial salt is added to the water to give a final conentration of 0–5 %, compressed air is bubbled through the water and the fish are kept in ‘salt water’ after transportation to decrease the incidence of skin infections.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1975.tb04619.x
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  • 2
    Electronic Resource
    Electronic Resource
    The surface area of mudfish (1976)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 8 (1976), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The surface area of two closely related mudfish species have been determined by coating the animals with masking tape. The results indicate that Labeo capensis specimens above 95 g body weight have a larger surface area than corresponding L. umbratus animals. The same is true for the fin surface area above 50 g body weight. Below these weights L. umbratus specimens have higher surface areas. The body surface area of L. capensis is always lower than that of L. umbratus in the range studied.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1976.tb03902.x
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  • 3
    Electronic Resource
    Electronic Resource
    The plasma proteins of Labeo umbratus (Smith) and Labeo capensis (Smith) (1974)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 6 (1974), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The plasma proteins of L. umbratus and L. capensis have been investigated by means of cellulose-acetate and polyacrylamide gel electrophoresis. Characteristic patterns were obtained for each specie although variations occur within a certain basic framework. Certain fractions were further isolated and approximate average molecular weights were found to vary between 55 000 and above 120 000 and isoelectric points between 5.78 and 4.2. Certain fractions were found to exist in dimeric form. It is concluded that the application of the human nomenclature to fish plasma protein fractions is of dubious value and that the fish fractions can only be classified after thorough biochemical investigation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1974.tb04559.x
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  • 4
    Electronic Resource
    Electronic Resource
    Electrophoresis of haemoglobin in single erythrocytes of Labeo umbratus (Smith) and Labeo capensis (Smith) (1974)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 6 (1974), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Electrophoresis of haemoglobin in single erythrocytes of Labeo umbratus and Labeo capensis showed that haemoglobin behaved in three distinct ways in both species. The majority of cells contained haemoglobin which moved as a broad band towards the anode. In a few cells haemoglobin moved towards both the anode and the cathode and in even fewer cells, haemoglobin moved as a sharp band to either the cathode or anode. These results are discussed in relation to other observations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1974.tb04562.x
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  • 5
    Electronic Resource
    Electronic Resource
    The haemoglobins of Labeo umbratus (Smith) and Labeo capensis (Smith) (1973)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 5 (1973), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The haemoglobins of both L. umbratus and L. capensis separated into four components on polyacrylamide electrophoresis. The isoelectric points of these fractions were found to be 9.35, 6.89, 6.63 and 6.40. Organic nucleotide triphosphate concentration in the blood of L. capensis was found to be 56.72 ± 18.01 mg% and 58.72 ± 17.9 mg% in the case of L. umbratus. It is not possible to distinguish between L. umbratus and L. capensis purely on the basis of the haemoglobin electropherogram.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1973.tb04483.x
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  • 6
    Electronic Resource
    Electronic Resource
    Partial separation of the pituitary proteins of Labeo umbratus, Smith (mudfish) (1973)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 5 (1973), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: L. umbratus pituitary glands were partially separated by means of preparative polyacry-lamide electrophoresis and chromatography on DEAE-cellulose and Sephadex G-50. Eight fractions were obtaìned and it was found that fraction 1 contained potent adrenocorticotropic stimulating activity, with a molecular weight in the region of 6000. Fractions 3 and 4 contained potent gonadotropic and a lesser degree of exopthalmic producing activity, with molecular weights in the region of 14 000 to 20 000. Fraction 6 displayed pigeon-crop stimulating activity, with a molecular weight above 30 000. Fractions 7 and 8 displayed M.S.H. and vasopressinoxytocic activity respectively, with a molecular weight in the region of 5500–6500. Fractions 2 and 5 could not be identified. The fractions seemed to be glycoproteins and the results are discussed in relation to previous findings.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1973.tb04429.x
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  • 7
    Electronic Resource
    Electronic Resource
    The quantification of individual proteins in crevicular gingival fluid (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 20 (1985), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The aim of the present study was the quantification of proteins present in crevicular fluid obtained from individual sulci, and to this effect simplified micromethods for the quantification of individual proteins were developed. Unstimulated, resting crevicular fluid from healthy crevicular spaces (i.e. in the absence of clinically detectable inflammation) of dogs and humans was collected for 3 minutes, either in microcapillary glass tubes or with the aid of filter paper strips. Quantification of proteins was done by radial immunodiffusion without the pooling of samples or by immunoelectrophoresis, without eluting from the papers. The concentrations of albumin and fibrinogen in crevicular fluid so determined proved to be significantly lower than plasma concentrations. These results strengthen the concept that capillary dynamics in healthy gingivae are similar to those in general tissue capillary beds and that gingival fluid is a plasma transudate. However, due to ever present sub-clinical inflammation and other factors, it is improbable that fluid from even healthy sulci is a true transudate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1985.tb00826.x
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  • 8
    Electronic Resource
    Electronic Resource
    Haematological assessment of generally used freshwater fish blood anticoagulants (1980)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 17 (1980), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Heparin injection B.P., containing 0·5% phenol as preservative, disodium ethylenediamine tetra-acetate (EDTA), trisodium citrate (TSC) and ammonium potassium oxalate (APO) were compared as anticoagulants for routine haematological investigations in Cyprinus carpio and Sarotherodon mossambicus. The acidic nature and haemolytic effects of EDTA and APO render them unsuitable for use with fish blood. TSC was required in higher concentrations (than heparin) for effective anticoagulation and this was undesirable for the determination of blood pH and pCO2 and certain other parameters. Several important interspecies differences in the reactions towards the anticoagulants are also reported. In general, heparin proved to be the anticoagulant of choice, although unsuitable for some assays.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1980.tb02768.x
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  • 9
    Electronic Resource
    Electronic Resource
    The effect of tricaine methanesulphonate (MS-222) on the microhaematocrit of fish blood (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 10 (1977), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The effects of the anaesthetic, MS-222, on the microhaematocrit value of freshwater fish have been examined. Blood containing MS-222 showed a higher haematocrit value than blood without the anaesthetic and haemolysis occurred in the former after a variable time depending on the concentration. The results are discussed in relation to previous findings.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1977.tb04077.x
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  • 10
    Electronic Resource
    Electronic Resource
    A seasonal study of the haematology of wild freshwater fish (1978)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 13 (1978), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Various haematological values of wild yellowfish (Barbus holubi), carp (Cyprinus carpio) and two species of mudfish (Labeo umbratus and Labeo capensis) were examined on a seasonal basis from May 1975-April 1976, obtained from three different localities. Haematological values of fish of different length groups (from all four species) were studied at one locality during winter only. (June 1976-August 1976). Seasonal variations were observed in all of the parameters studied but no sexual differences were found and no significant changes in the haematology could be related to breeding season. Wide individual variations were evident in fish of different length groups and no correlation existed between fish length (and mass) and haematological values.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1978.tb03438.x
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