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  • Electronic Resource  (14)
  • 1
    Electronic Resource
    Electronic Resource
    Sequential Polypeptides. 2. Helix-Coil Transition in Poly(γ-benzyl iε-carbobenzoxy-L-lysyl-L-glutamate) (1977)
    s.l. : American Chemical Society
    Macromolecules 10 (1977), S. 1061-1065 
    Details
    ISSN: 1520-5835
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ma60059a033
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Perforated-Plate Distillation Columns (1952)
    s.l. : American Chemical Society
    Industrial & engineering chemistry 44 (1952), S. 2238-2249 
    Details
    ISSN: 1520-5045
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ie50513a070
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  • 3
    Electronic Resource
    Electronic Resource
    Pathogen safety of manufacturing processes for biological products: special emphasis on KOGENATE® Bayer (2002)
    Oxford, UK : Blackwell Science, Ltd
    Haemophilia 8 (2002), S. 0 
    Details
    ISSN: 1365-2516
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary.  Manufacturers of human therapeutic proteins derived from biological sources continuously strive to improve the pathogen safety profiles of these products. Efforts to improve pathogen safety margins for these biological products are directed towards several areas within the manufacturing processes including: (a) sourcing and screening of raw materials (b) determining the potential for manufacturing processes to reduce pathogen titres, and (c) incorporating methods designed specifically to remove or inactivate contaminating pathogens. Methods that could potentially reduce pathogen titres are a major focus for many manufacturers. In general, these methods are grouped into two categories, pathogen clearance and pathogen inactivation. Assessments are performed on small-scale, laboratory simulations of the manufacturing process of interest that are spiked with a known amount of a selected pathogen. These studies provide estimates of the potential for a process step to remove or inactivate a particular pathogen. There are several pathogen clearance/inactivation methods that are inherent in manufacturing processes, however, some methods are intentionally incorporated into manufacturing for the sole purpose of reducing putative pathogen titres. Not only are well-known pathogens such as viruses targeted, but also suspected pathogens such as those associated with the transmissible spongiform encephalopathies (TSEs). The production processes for the isolation of several biological products, including recombinant KOGENATE® Bayer (Kogenate®FS), have been evaluated for the ability to reduce pathogen titres and/or have been designed to incorporate methods for reducing potential pathogen safety risks. Several processing steps with the potential to reduce pathogen titres have been identified.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1351-8216.2001.00117.x
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  • 4
    Electronic Resource
    Electronic Resource
    Bacterial enteritis and the development of the larval digestive tract in olive flounder, Paralichthys olivaceus (Temminck & Schlegel) (2004)
    Oxford, UK : Blackwell Science Ltd
    Journal of fish diseases 27 (2004), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Three bacterial isolates obtained from diseased olive flounder larvae, Paralichthys olivaceus, were identified as Vibrio ichthyoenteri based on the results of phenotypic characterization and 16S rRNA gene sequencing studies. Bacterial enteritis was reproduced in 16 and 22 days post-hatch (dph) larvae by administering brine shrimp nauplii, Artemia salina, dosed with the environmental isolates and reference strains of V. ichthyoenteri. To investigate the effect of the disease on development of the stomach, a pepsin activity assay and reverse transcription-polymerase chain reaction (RT-PCR) analysis of the expression of the pepsinogen gene were performed. Expression of olive flounder pepsinogen was detected from 30-dph larvae and the increased level of pepsin activity coincided with reduced susceptibility to the disease. Growth rates of V. ichthyoenteri, V. anguillarum and Edwardsiella tarda were tested in artificial stomach conditions using HCl and porcine pepsin. All the strains of V. ichthyoenteri were inhibited by low pH conditions which corresponded with an increase in pepsin levels. This suggests that differentiation of the stomach in olive flounder larvae and juveniles, an essential physiological development, also provides the host with a non-immunological defence mechanism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2761.2004.00553.x
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  • 5
    Electronic Resource
    Electronic Resource
    Isotopic variations with distance and time in the volcanic islands of the Cameroon line: evidence for a mantle plume origin
    Amsterdam : Elsevier
    Earth and Planetary Science Letters 123 (1994), S. 119-138 
    Details
    ISSN: 0012-821X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Geosciences , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0012-821X(94)90262-3
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  • 6
    Electronic Resource
    Electronic Resource
    Study of rubber-modified epoxy resin using thermally stimulated current (1998)
    Springer
    Polymer bulletin 40 (1998), S. 599-605 
    Details
    ISSN: 1436-2449
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Summary In order to characterize the low temperature relaxation of epoxy resin modified with amine-terminated butadiene acrylonitrile copolymers (ATBN), thermally stimulated current (TSC) and relaxation map analysis (RMA) were investigated. Four relaxation peaks at around −140, −100, −60 and 0°C were observed as the indication of γ-, β-relaxation of epoxy resin, Tg, new unknown peak of ATBN, respectively. The unknown peak at around 0°C was observed due to dipole orientation. The RMA data was showed that compensation temperature (Tc) and degree-of-disorder (DOD) were increased with increasing the content of acrylonitrile and ATBN, whereas the compensation time (τc) was decreased. It can be concluded that the cooperative molecular motion in cured epoxy resin was more active as the concentration of acrylonitrile and ATBN content increases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002890050296
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  • 7
    Electronic Resource
    Electronic Resource
    Study of siloxane-modified epoxy resin using thermally stimulated current(II) (1997)
    Springer
    Polymer bulletin 39 (1997), S. 193-199 
    Details
    ISSN: 1436-2449
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract. Thermally stimulated current (TSC) and relaxation map analysis (RMA) was used to characterize the low temperature relaxation of epoxy resin modified with siloxane oligomers. In aminopropyl-terminated siloxane oligomer (ATSO) the β-relaxation of epoxy resin and the glass transition temperature of siloxane oligomer were folded regardless of the concentration of diphenyl. The β-relaxation of epoxy resin and the glass transition temperature of oxiranylmethoxy-terminated siloxane oligomer (OTSO) were folded and shifted to higher temperature as the concentration of diphenyl in siloxane oligomer increased. In the systems containing of diphenyl in siloxane oligomer a new relaxation peak due to the space charge was observed in the range of − 80 °C to − 50 °C and − 30 °C to 5 °C. As the concentration of diphenyl increased the compensation temperature (Tc) and the degree-of-disorder (DOD) were increased while the compensation time, τ c was decreased.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002890050138
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  • 8
    Electronic Resource
    Electronic Resource
    Thermostable d-hydantoinase from thermophilic Bacillus stearothermophilus SD-1: characteristics of purified enzyme (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 270-276 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract One thousand thermophiles isolated from soils were screened for hydantoinase and its thermostability. One thermophilic bacterium that showed the highest thermostability and activity of hydantoinase was identified to be Bacillus stearothermophilus SD-1 according to morphological and physiological characteristics. The hydantoinase of B. stearothermophilus SD-1 was purified to homogeneity via ammonium sulfate fractionation, anion-exchange chromatography, heat treatment, hydrophobic-interaction chromatography, and preparative gel electrophoresis. The relative molecular mass of the hydantoinase was determined to be 126 kDa by gel-filtration chromatography, and a value of 54 kDa was obtained as a molecular mass of the subunit on analytical sodiumdodecylsulfate/polyacrylamide gel electrophoresis. The hydantoinase was strictly d-specific and metal-dependent. The optimal pH and temperature were about 8.0 and 65°C respectively, and the half-life of the d-hydantoinase was estimated to be 30 min at 80°C, indicating the most thermostable enzyme so far.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00172823
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  • 9
    Electronic Resource
    Electronic Resource
    Thermostable d-hydantoinase from thermophilic Bacillus stearothermophilus SD-1: characteristics of purified enzyme (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 270-276 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  One thousand thermophiles isolated from soils were screened for hydantoinase and its thermostability. One thermophilic bacterium that showed the highest thermostability and activity of hydantoinase was identified to be Bacillus stearothermophilus SD-1 according to morphological and physiological characteristics. The hydantoinase of B. stearothermophilus SD-1 was purified to homogeneity via ammonium sulfate fractionation, anion-exchange chromatography, heat treatment, hydrophobic-interaction chromatography and preparative gel electrophoresis. The relative molecular mass of the hydantoinase was determined to be 126 kDa by gel-filtration chromatography, and a value of 54 kDa was obtained as a molecular mass of the subunit on analytical sodiumdodecylsulfate/polyacrylamide gel electrophoresis. The hydantoinase was strictly d-specific and metal-dependent. The optimal pH and temperature were about 8.0 and 65°  C respectively, and the half-life of the d-hydantoinase was estimated to be 30 min at 80°  C, indicating the most thermostable enzyme so far.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050401
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  • 10
    Electronic Resource
    Electronic Resource
    Screening of hexavalent chromium biosorbent from marine algae (2000)
    Springer
    Applied microbiology and biotechnology 54 (2000), S. 445-448 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A high-chromate-selective biosorbent with high adsorption capacity was sought by examining the chromate adsorption capacities of 48 species of red, brown, or green marine algae sampled from the east coast of Korea. Screening showed a red marine alga to have the most excellent adsorption characteristics among them, and it was identified as Pachymeniopsis sp. The period at which Pachymeniopsis sp. was sampled did not affect the adsorption capacity of the alga, but the optimum period for mass collection was April to May. The alga also showed high selectivity for chromate since its adsorption capacity for other heavy metal ions such as cadmium and manganese ions was relatively low. An investigation of the adsorption isotherm of dried powder of Pachymeniopsis sp. for chromate adsorption at 25 °C showed a Langmuir-type dependence. The maximum chromate adsorption capacity of the selected alga was about 225 mg/g. Desorption of the adsorbed chromate from Pachymeniopsis sp. was done by treating the sample with 1 N NaOH. It was confirmed that ion exchange type adsorption was observed with an anion exchanger but not with a cation exchanger. It is therefore believed that the chromate adsorption is based on anionic exchange of Pachymeniopsis sp.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530000387
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